Construct: ORF TRCN0000477492

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016043.1_s317c1
Derived from:
ccsbBroadEn_13285
DNA Barcode:
AAGCTATCTCTCTTCCCTAAGAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NEK10 (152110)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477492
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 152110 NEK10 NIMA related kinase 10 NM_001031741.3 99.8% 100% 183C>G;846C>T
2 human 152110 NEK10 NIMA related kinase 10 NM_001304384.1 97.7% 97.9% 183C>G;846C>T;946_975del
3 human 152110 NEK10 NIMA related kinase 10 XM_024453374.1 55.4% 55.2% (many diffs)
4 human 152110 NEK10 NIMA related kinase 10 XM_017005768.1 41.5% 41.3% (many diffs)
5 human 152110 NEK10 NIMA related kinase 10 XM_017005767.2 40.9% 40.7% (many diffs)
6 human 152110 NEK10 NIMA related kinase 10 XM_024453373.1 40.9% 40.7% (many diffs)
7 human 152110 NEK10 NIMA related kinase 10 XM_006712997.3 40.2% 40.1% (many diffs)
8 human 152110 NEK10 NIMA related kinase 10 XM_017005766.2 40.2% 40.1% (many diffs)
9 human 152110 NEK10 NIMA related kinase 10 NM_152534.4 39.9% 39.7% (many diffs)
10 human 152110 NEK10 NIMA related kinase 10 XM_017005761.2 39.9% 39.7% (many diffs)
11 human 152110 NEK10 NIMA related kinase 10 XM_017005762.2 39.9% 39.7% (many diffs)
12 human 152110 NEK10 NIMA related kinase 10 XM_017005763.1 39.9% 39.7% (many diffs)
13 human 152110 NEK10 NIMA related kinase 10 XM_017005764.1 39.9% 39.7% (many diffs)
14 human 152110 NEK10 NIMA related kinase 10 XM_017005765.1 39.9% 39.7% (many diffs)
15 human 152110 NEK10 NIMA related kinase 10 XM_006712998.2 38.9% 38.4% (many diffs)
16 human 152110 NEK10 NIMA related kinase 10 XM_006712999.3 38.7% 38.3% (many diffs)
17 human 152110 NEK10 NIMA related kinase 10 XM_017005772.2 37.9% 37.3% (many diffs)
18 human 152110 NEK10 NIMA related kinase 10 XM_017005769.1 37% 36.3% (many diffs)
19 human 152110 NEK10 NIMA related kinase 10 XM_017005770.2 37% 36.3% (many diffs)
20 human 152110 NEK10 NIMA related kinase 10 XM_006713001.3 36.2% 36% (many diffs)
21 human 152110 NEK10 NIMA related kinase 10 XM_017005771.2 36.2% 36% (many diffs)
22 human 152110 NEK10 NIMA related kinase 10 XM_017005773.1 21.6% 21.3% (many diffs)
23 human 152110 NEK10 NIMA related kinase 10 XR_001740034.1 21.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1488
ORF length:
1422
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt tcctgtacag cctgcagaac cccccgaggt actgaagagt gagccgtatg
121gggagaaggc tgatgtctgg gcagtaggct gcatccttta tcagatggcg actttgagtc
181cccccttcta cagcactaac atgctgtcct tggctacaaa aatagtggag gcggtatatg
241aaccagtgcc agaaggtatc tactctgaaa aagtaacaga caccatcagc aggtgcctca
301ctcctgatgc ggaagctcgt ccagatattg tagaagtcag ttcgatgata tcagatgtca
361tgatgaaata tttagacaac ttatctacat cccagttgtc cttggaaaag aagctagaac
421gggaacgaag acgcacacaa aggtatttta tggaagccaa ccggaacacc gtcacatgtc
481accatgagct ggctgttcta tctcacgaga cctttgagaa ggcaagtttg agtagcagca
541gcagtggagc agccagcctg aaaagtgaac tttcagaaag cgcagacctg ccccctgaag
601gcttccaggc ctcctatggt aaagacgaag acagggcctg tgacgaaatc ctgtcagatg
661ataacttcaa cctggaaaat gctgagaaag atacatattc agaggtagat gatgaattgg
721acatttcgga taactccagc agctccagtt caagccctct gaaagaatct acattcaaca
781ttttaaagag aagttttagt gcttcaggag gagaaagaca atcccaaaca agggacttca
841ctggaggaac aggatcaaga ccaagaccag ctttgctgcc tcttgacctg cttctgaaag
901tgccacccca tatgctcagg gcccacatta aggaaataga ggctgagtta gtgacagggt
961ggcagtccca tagccttcct gctgtgattc ttcgaaatct caaagatcat gcatcagcag
1021gaattgctgt gtcccagagg aaagtgcgtc agatcagtga tcctattcag cagatattaa
1081ttcagctgca caaaataatc tatatcacac agcttcctcc agctttgcac cacaatttga
1141aaagaagggt tatagagaga ttcaagaaat ccctcttcag ccagcagagt aacccttgta
1201attTGAAATC TGAAATTAAA AAGTTATCTC AGGGATCTCC AGAACCGATT GAGCCCAACT
1261TTTTCACAGC AGATTACCAT TTATTACATC GTTCATCCGG TGGAAACAGC CTGTCCCCAA
1321ATGACCCTAC AGGTTTACCA ACCAGCATTG AATTGGAGGA AGGAATAACA TATGAACAGA
1381TGCAGACTGT GATTGAAGAA GTCCTTGAGG AAAGTGGCTA TTACAATTTT ACATCTAACA
1441GGTATCATTC CTATCCATGG GGGACCAAGA ATCACCCAAC CAAAAGATGC CCAACTTTCT
1501TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1561CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1621GTGGAAAGGA CGAAAGCTAT CTCTCTTCCC TAAGAAGACG CGTTAAGTCg acaatcaacc
1681tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)