Construct: ORF TRCN0000477535

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003327.1_s317c1
Derived from:
ccsbBroadEn_01899
DNA Barcode:
GAGTATGATTCTCGACACACTATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
H2BC14 (8342)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477535
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8342 H2BC14 H2B clustered histone 14 NM_003521.2 100% 100%
2 human 3017 H2BC5 H2B clustered histone 5 NM_021063.3 89.4% 96.8% (many diffs)
3 human 3017 H2BC5 H2B clustered histone 5 NM_138720.2 89.4% 96.8% (many diffs)
4 human 8341 H2BC15 H2B clustered histone 15 NM_003520.3 88.9% 96.8% (many diffs)
5 human 8340 H2BC13 H2B clustered histone 13 NM_003519.3 88.8% 95.2% (many diffs)
6 human 8344 H2BC6 H2B clustered histone 6 NM_003523.2 88.6% 96.8% (many diffs)
7 human 8346 H2BC10 H2B clustered histone 10 NM_003525.2 88.6% 96.8% (many diffs)
8 human 8347 H2BC4 H2B clustered histone 4 NM_003526.2 88.6% 96.8% (many diffs)
9 human 85236 H2BC12 H2B clustered histone 12 NM_001312653.1 88.6% 96% (many diffs)
10 human 85236 H2BC12 H2B clustered histone 12 NM_080593.2 88.6% 96% (many diffs)
11 human 8348 H2BC17 H2B clustered histone 17 NM_003527.4 88.6% 95.2% (many diffs)
12 human 8349 H2BC21 H2B clustered histone 21 NM_003528.2 88.3% 96% (many diffs)
13 human 440689 H2BC18 H2B clustered histone 18 NM_001024599.4 87.8% 95.2% (many diffs)
14 human 8343 H2BC7 H2B clustered histone 7 NM_003522.3 87.8% 96.8% (many diffs)
15 human 54145 H2BS1 H2B.S histone 1 NM_017445.3 87.8% 94.4% (many diffs)
16 human 102724334 LOC102724334 histone H2B type F-S-like XM_006723918.4 87.8% 94.4% (many diffs)
17 human 8345 H2BC9 H2B clustered histone 9 NM_003524.2 87.5% 96% (many diffs)
18 human 8970 H2BC11 H2B clustered histone 11 NM_021058.3 86.2% 95.2% (many diffs)
19 human 3018 H2BC3 H2B clustered histone 3 NM_021062.2 86% 96.8% (many diffs)
20 human 128312 H2BU1 H2B.U histone 1 NM_175055.2 84.9% 92.8% (many diffs)
21 human 8339 H2BC8 H2B clustered histone 8 NM_003518.3 84.9% 96.8% (many diffs)
22 human 440689 H2BC18 H2B clustered histone 18 NM_001161334.1 82.6% 89.5% (many diffs)
23 human 3017 H2BC5 H2B clustered histone 5 XM_005249039.4 75.1% 81.3% (many diffs)
24 human 337875 H2BP1 H2B histone pseudogene 1 NR_160944.2 66.5% (many diffs)
25 human 337873 H2BC20P H2B clustered histone 20, p... NR_036461.1 56.1% (many diffs)
26 human 338391 H2BP2 H2B histone pseudogene 2 NR_160915.1 20.6% (many diffs)
27 human 337875 H2BP1 H2B histone pseudogene 1 NR_027337.2 18% (many diffs)
28 human 106479023 H3P4 H3 histone pseudogene 4 NR_160941.1 16.9% (many diffs)
29 human 337875 H2BP1 H2B histone pseudogene 1 NR_160942.1 15.5% (many diffs)
30 human 106479023 H3P4 H3 histone pseudogene 4 NR_160940.1 15% (many diffs)
31 human 338391 H2BP2 H2B histone pseudogene 2 NR_160913.1 13.4% (many diffs)
32 human 338391 H2BP2 H2B histone pseudogene 2 NR_160914.1 11% (many diffs)
33 mouse 319179 Hist1h2be histone cluster 1, H2be NM_001177653.1 88.6% 96.8% (many diffs)
34 mouse 68024 Hist1h2bc histone cluster 1, H2bc NM_001290380.1 88.6% 96.8% (many diffs)
35 mouse 319179 Hist1h2be histone cluster 1, H2be NM_001290530.1 88.6% 96.8% (many diffs)
36 mouse 68024 Hist1h2bc histone cluster 1, H2bc NM_023422.3 88.6% 96.8% (many diffs)
37 mouse 319179 Hist1h2be histone cluster 1, H2be NM_178194.4 88.6% 96.8% (many diffs)
38 mouse 319182 Hist1h2bh histone cluster 1, H2bh NM_178197.2 88.6% 96.8% (many diffs)
39 mouse 665622 Hist1h2br histone cluster 1 H2br NM_001313878.1 88.3% 96% (many diffs)
40 mouse 665596 Hist1h2bq histone cluster 1, H2bq NM_001313880.1 88.3% 96% (many diffs)
41 mouse 319187 Hist1h2bn histone cluster 1, H2bn NM_178201.2 88.3% 96% (many diffs)
42 mouse 319181 Hist1h2bg histone cluster 1, H2bg NM_178196.4 88% 96.8% (many diffs)
43 mouse 319186 Hist1h2bm histone cluster 1, H2bm NM_178200.2 88% 96.8% (many diffs)
44 mouse 319188 Hist1h2bp histone cluster 1, H2bp NM_001290466.1 88% 96% (many diffs)
45 mouse 319185 Hist1h2bl histone cluster 1, H2bl NM_178199.2 88% 96% (many diffs)
46 mouse 319188 Hist1h2bp histone cluster 1, H2bp XM_006516690.2 88% 96% (many diffs)
47 mouse 319184 Hist1h2bk histone cluster 1, H2bk NM_175665.2 88% 95.2% (many diffs)
48 mouse 319180 Hist1h2bf histone cluster 1, H2bf NM_178195.2 87.8% 96% (many diffs)
49 mouse 319183 Hist1h2bj histone cluster 1, H2bj NM_178198.2 87.8% 96% (many diffs)
50 mouse 319178 Hist1h2bb histone cluster 1, H2bb NM_175664.3 87.5% 96.8% (many diffs)
51 mouse 319190 Hist2h2be histone cluster 2, H2be NM_178214.4 85.4% 92.8% (many diffs)
52 mouse 78303 Hist3h2ba histone cluster 3, H2ba NM_030082.4 84.8% 92.8% (many diffs)
53 mouse 319189 Hist2h2bb histone cluster 2, H2bb NM_175666.2 84.3% 95.2% (many diffs)
54 mouse 665596 Hist1h2bq histone cluster 1, H2bq NM_001097979.2 82.8% 89.5% (many diffs)
55 mouse 665622 Hist1h2br histone cluster 1 H2br NM_001110555.1 82.8% 89.5% (many diffs)
56 mouse 319177 Hist1h2ba histone cluster 1, H2ba NM_175663.2 81.4% 85.8% (many diffs)
57 mouse 319188 Hist1h2bp histone cluster 1, H2bp NM_178202.2 80.1% 87.6% (many diffs)
58 mouse 382522 Hist3h2bb-ps histone cluster 3, H2bb, ps... NR_134309.1 69.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
447
ORF length:
378
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttGGCACCAT GCCTGAACCA GTCAAATCTG CTCCAGTCCC TAAAAAAGGC TCCAAGAAGG
121CCATTAACAA GGCTCAGAAG AAGGATGGAA AGAAGCGCAA ACGCAGCCGC AAGGAGAGCT
181ACTCTGTGTA TGTGTACAAG GTGCTGAAGC AGGTCCACCC CGACACCGGC ATCTCTTCCA
241AGGCTATGGG AATCATGAAC TCCTTCGTCA ACGACATCTT TGAGCGTATC GCCGGAGAAG
301CGTCACGCCT GGCGCATTAC AACAAGCGCT CGACCATCAC TTCGAGGGAG ATCCAGACGG
361CCGTGCGCCT ACTGCTACCC GGGGAATTGG CCAAGCACGC CGTGTCCGAG GGCACCAAGG
421CCGTCACCAA GTATACCAGC TCCAAGTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
481GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
541AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAGTATGA
601TTCTCGACAC ACTATAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
661aagatt

Download FASTA (ORF) (Full)