Construct: ORF TRCN0000477544
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002617.1_s317c1
- Derived from:
- ccsbBroadEn_01355
- DNA Barcode:
- TTAGAGCATCTCGACTCCTCAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PYGL (5836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477544
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5836 | PYGL | glycogen phosphorylase L | NM_002863.5 | 100% | 100% | |
| 2 | human | 5836 | PYGL | glycogen phosphorylase L | NM_001163940.2 | 95.9% | 95.9% | 241_242ins102 |
| 3 | mouse | 110095 | Pygl | liver glycogen phosphorylase | NM_133198.2 | 88.7% | 94.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2610
- ORF length:
- 2541
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgaagccc ctgacggacc aggagaagcg gcggcagatc agcatccgcg 121 gcatcgtggg cgtggagaac gtggcagagc tgaagaagag tttcaaccgg cacctgcact 181 tcacgctggt caaggaccgc aacgtggcca ccacccgcga ctactacttc gcgctggcgc 241 acacggtgcg cgaccacctg gtggggcgct ggatccgcac gcagcagcac tactacgaca 301 agtgccccaa gagggtatat tacctctctc tggaatttta catgggccga acattacaga 361 acaccatgat caacctcggt ctgcaaaatg cctgtgatga ggccatttac cagcttggat 421 tggatataga agagttagaa gaaattgaag aagatgctgg acttggcaat ggtggtcttg 481 ggagacttgc tgcctgcttc ttggattcca tggcaaccct gggacttgca gcctatggat 541 acggcattcg gtatgaatat gggattttca atcagaagat ccgagatgga tggcaggtag 601 aagaagcaga tgattggctc agatatggaa acccttggga gaagtcccgc ccagaattca 661 tgctgcctgt gcacttctat ggaaaagtag aacacaccaa caccgggacc aagtggattg 721 acactcaagt ggtcctggct ctgccatatg acacccccgt gcccggctac atgaataaca 781 ctgtcaacac catgcgcctc tggtctgctc gggcaccaaa tgactttaac ctcagagact 841 ttaatgttgg agactacatt caggctgtgc tggaccgaaa cctggccgag aacatctccc 901 gggtcctcta tcccaatgac aatttttttg aagggaagga gctaagattg aagcaggaat 961 actttgtggt ggctgcaacc ttgcaagata tcatccgccg tttcaaagcc tccaagtttg 1021 gctccacccg tggtgcagga actgtgtttg atgccttccc ggatcaggtg gccatccagc 1081 tgaatgacac tcaccctgca ctcgcgatcc ctgagctgat gaggattttt gtggatattg 1141 aaaaactgcc ctggtccaag gcatgggagc tcacccagaa gaccttcgcc tacaccaacc 1201 acacagtgct cccggaagcc ctggagcgct ggcccgtgga cctggtggag aagctgctcc 1261 ctcgacattt ggaaatcatt tatgagataa atcagaagca tttagataga attgtggcct 1321 tgtttcctaa agatgtggac cgtctgagaa ggatgtctct gatagaagag gaaggaagca 1381 aaaggatcaa catggcccat ctctgcattg tcggttccca tgctgtgaat ggcgtggcta 1441 aaatccactc agacatcgtg aagactaaag tattcaagga cttcagtgag ctagaacctg 1501 acaagtttca gaataaaacc aatgggatca ctccaaggcg ctggctccta ctctgcaacc 1561 caggacttgc agagctcata gcagagaaaa ttggagaaga ctatgtgaaa gacctgagcc 1621 agctgacgaa gctccacagc ttcctgggtg atgatgtctt cctccgggaa ctcgccaagg 1681 tgaagcagga gaataagctg aagttttctc agttcctgga gacggagtac aaagtgaaga 1741 tcaacccatc ctccatgttt gatgtccagg tgaagaggat acatgagtac aagcgacagc 1801 tcttgaactg tctgcatgtg atcacgatgt acaaccgcat taagaaagac cctaagaagt 1861 tattcgtgcc aaggacagtt atcattggtg gtaaagctgc cccaggatat cacatggcca 1921 aaatgatcat aaagctgatc acttcagtgg cagatgtggt gaacaatgac cctatggttg 1981 gaagcaagtt gaaagtcatc ttcttggaga actacagagt atctcttgct gaaaaagtca 2041 ttccagccac agatctgtca gagcagattt ccactgcagg caccgaagcc TCGGGGACAG 2101 GCAATATGAA GTTCATGCTA AATGGGGCCC TAACTATCGG GACCATGGAT GGGGCCAATG 2161 TGGAAATGGC AGAAGAAGCT GGGGAAGAGA ACCTGTTCAT CTTTGGCATG AGGATAGATG 2221 ATGTGGCTGC TTTGGACAAG AAAGGGTACG AGGCAAAAGA ATACTATGAG GCACTTCCAG 2281 AGCTGAAGCT GGTCATTGAT CAAATTGACA ATGGCTTTTT TTCTCCCAAG CAGCCTGACC 2341 TCTTCAAAGA TATCATCAAC ATGCTATTTT ATCATGACAG GTTTAAAGTC TTTGCAGACT 2401 ACGAAGCCTA TGTCAAGTGT CAAGATAAAG TGAGTCAGCT GTACATGAAT CCAAAGGCCT 2461 GGAACACAAT GGTACTCAAA AACATAGCTG CCTCGGGGAA ATTCTCCAGT GACCGAACAA 2521 TTAAAGAATA TGCCCAAAAC ATCTGGAACG TGGAACCTTC AGATCTAAAG ATTTCTCTAT 2581 CCAATGAATC TAACAAAGTC AATGGAAATT TGCCAACTTT CTTGTACAAA GTGGTTGATA 2641 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2701 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTAGA 2761 GCATCTCGAC TCCTCAAGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2821 tgaaagatt