Construct: ORF TRCN0000477552

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006320.1_s317c1
Derived from:
ccsbBroadEn_04248
DNA Barcode:
CAAGAGCGCCTAAAGGCCGCTAAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UNC93B1 (81622)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477552
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81622 UNC93B1 unc-93 homolog B1, TLR sign... NM_030930.4 100% 100%
2 human 81622 UNC93B1 unc-93 homolog B1, TLR sign... XM_011545290.1 77% 77% 0_1ins411
3 human 81622 UNC93B1 unc-93 homolog B1, TLR sign... XM_011545291.2 69% 69% 0_1ins555
4 mouse 54445 Unc93b1 unc-93 homolog B1 (C. elegans) NM_019449.2 82% 87.4% (many diffs)
5 mouse 54445 Unc93b1 unc-93 homolog B1 (C. elegans) XM_006531798.1 74.1% 78.7% (many diffs)
6 mouse 54445 Unc93b1 unc-93 homolog B1 (C. elegans) NM_001161428.1 56.8% 57.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1860
ORF length:
1791
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaggcggag ccgccgctct acccgatggc gggggctgcg gggccgcagg
121gcgacgagga cctgctcggg gtcccggacg ggcccgaggc cccgctggac gagctggtgg
181gcgcgtaccc caactacaac gaggaggagg aggagcgccg ctactaccgc cgcaagcgcc
241tgggcgtgct caagaacgtg ctggctgcca gcgccggggg catgctcacc tacggcgtct
301acctgggcct cctgcagatg cagctgatcc tgcactacga cgagacctac cgcgaggtga
361agtatggcaa catggggctg cccgacatcg acagcaaaat gctgatgggc atcaacgtga
421ctcccatcgc cgccctgctc tacacacctg tgctcatcag gttttttgga acgaagtgga
481tgatgttcct cgctgtgggc atctacgccc tctttgtctc caccaactac tgggagcgct
541actacacgct tgtgccctcg gctgtggccc tgggcatggc catcgtgcct ctttgggctt
601ccatgggcaa ctacatcacc aggatggcgc agaagtacca tgagtactcc cactacaagg
661agcaggatgg gcaggggatg aagcagcggc ctccgcgggg ctcccacgcg ccctatctcc
721tggtcttcca agccatcttc tacagcttct tccatctgag cttcgcctgc gcccagctgc
781ccatgattta tttcctgaac cactacctgt atgacctgaa ccacacgctg tacaatgtgc
841agagctgcgg caccaacagc cacgggatcc tcagcggctt caacaagacg gttctgcgga
901cgctcccgcg gagcggaaac ctcattgtgg tggagagcgt gctcatggca gtggccttcc
961tggccatgct gctggtgctg ggtttgtgcg gagccgctta ccggcccacg gaggagatcg
1021atctgcgcag cgtgggctgg ggcaacatct tccagctgcc cttcaagcac gtgcgtgact
1081accgcctgcg ccacctcgtg cctttcttta tctacagcgg cttcgaggtg ctctttgcct
1141gcactggtat cgccttgggc tatggcgtgt gctcggtggg gctggagcgg ctggcttacc
1201tcctcgtggc ttacagcctg ggcgcctcag ccgcctcact cctgggcctg ctgggcctgt
1261ggctgccacg cccggtgccc ctggtggccg gagcaggggt gcacctgctg ctcaccttca
1321tcctcttttt ctgggcccct gtgcctcggg tcctgcaaca cagctggatc ctctatgtgg
1381cagctgccct ttggggtgtg ggcagtgccc tgaacaagac tggactcagc acactcctgg
1441gaatcttgta cgaagacaag gagagacagg acttcatctt caccatctac cactggtggc
1501aggctgtggc catcttcacc gtgtacctgg gctcgagcct gcacatgaag gctaagctGG
1561CGGTGCTGCT GGTGACGCTG GTGGCGGCCG CGGTCTCCTA CCTGCGGATG GAGCAGAAGC
1621TGCGCCGGGG CGTGGCCCCG CGCCAGCCCC GCATCCCGCG GCCCCAGCAC AAGGTGCGCG
1681GTTACCGCTA CTTGGAGGAG GACAACTCGG ACGAGAGCGA CGCGGAGGGC GAGCATGGGG
1741ACGGCGCGGA GGAGGAGGCG CCGCCCGCAG GGCCCAGGCC TGGCCCCGAG CCCGCTGGAC
1801TCGGCCGCCG GCCCTGCCCG TACGAACAGG CGCAGGGGGG AGACGGGCCG GAGGAGCAGT
1861TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1921GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1981TTTATATATC TTGTGGAAAG GACGACAAGA GCGCCTAAAG GCCGCTAACA CGCGTTAAGT
2041Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)