Construct: ORF TRCN0000477552
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006320.1_s317c1
- Derived from:
- ccsbBroadEn_04248
- DNA Barcode:
- CAAGAGCGCCTAAAGGCCGCTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UNC93B1 (81622)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477552
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81622 | UNC93B1 | unc-93 homolog B1, TLR sign... | NM_030930.4 | 100% | 100% | |
| 2 | human | 81622 | UNC93B1 | unc-93 homolog B1, TLR sign... | XM_011545290.1 | 77% | 77% | 0_1ins411 |
| 3 | human | 81622 | UNC93B1 | unc-93 homolog B1, TLR sign... | XM_011545291.2 | 69% | 69% | 0_1ins555 |
| 4 | mouse | 54445 | Unc93b1 | unc-93 homolog B1 (C. elegans) | NM_019449.2 | 82% | 87.4% | (many diffs) |
| 5 | mouse | 54445 | Unc93b1 | unc-93 homolog B1 (C. elegans) | XM_006531798.1 | 74.1% | 78.7% | (many diffs) |
| 6 | mouse | 54445 | Unc93b1 | unc-93 homolog B1 (C. elegans) | NM_001161428.1 | 56.8% | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1860
- ORF length:
- 1791
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcggag ccgccgctct acccgatggc gggggctgcg gggccgcagg 121 gcgacgagga cctgctcggg gtcccggacg ggcccgaggc cccgctggac gagctggtgg 181 gcgcgtaccc caactacaac gaggaggagg aggagcgccg ctactaccgc cgcaagcgcc 241 tgggcgtgct caagaacgtg ctggctgcca gcgccggggg catgctcacc tacggcgtct 301 acctgggcct cctgcagatg cagctgatcc tgcactacga cgagacctac cgcgaggtga 361 agtatggcaa catggggctg cccgacatcg acagcaaaat gctgatgggc atcaacgtga 421 ctcccatcgc cgccctgctc tacacacctg tgctcatcag gttttttgga acgaagtgga 481 tgatgttcct cgctgtgggc atctacgccc tctttgtctc caccaactac tgggagcgct 541 actacacgct tgtgccctcg gctgtggccc tgggcatggc catcgtgcct ctttgggctt 601 ccatgggcaa ctacatcacc aggatggcgc agaagtacca tgagtactcc cactacaagg 661 agcaggatgg gcaggggatg aagcagcggc ctccgcgggg ctcccacgcg ccctatctcc 721 tggtcttcca agccatcttc tacagcttct tccatctgag cttcgcctgc gcccagctgc 781 ccatgattta tttcctgaac cactacctgt atgacctgaa ccacacgctg tacaatgtgc 841 agagctgcgg caccaacagc cacgggatcc tcagcggctt caacaagacg gttctgcgga 901 cgctcccgcg gagcggaaac ctcattgtgg tggagagcgt gctcatggca gtggccttcc 961 tggccatgct gctggtgctg ggtttgtgcg gagccgctta ccggcccacg gaggagatcg 1021 atctgcgcag cgtgggctgg ggcaacatct tccagctgcc cttcaagcac gtgcgtgact 1081 accgcctgcg ccacctcgtg cctttcttta tctacagcgg cttcgaggtg ctctttgcct 1141 gcactggtat cgccttgggc tatggcgtgt gctcggtggg gctggagcgg ctggcttacc 1201 tcctcgtggc ttacagcctg ggcgcctcag ccgcctcact cctgggcctg ctgggcctgt 1261 ggctgccacg cccggtgccc ctggtggccg gagcaggggt gcacctgctg ctcaccttca 1321 tcctcttttt ctgggcccct gtgcctcggg tcctgcaaca cagctggatc ctctatgtgg 1381 cagctgccct ttggggtgtg ggcagtgccc tgaacaagac tggactcagc acactcctgg 1441 gaatcttgta cgaagacaag gagagacagg acttcatctt caccatctac cactggtggc 1501 aggctgtggc catcttcacc gtgtacctgg gctcgagcct gcacatgaag gctaagctGG 1561 CGGTGCTGCT GGTGACGCTG GTGGCGGCCG CGGTCTCCTA CCTGCGGATG GAGCAGAAGC 1621 TGCGCCGGGG CGTGGCCCCG CGCCAGCCCC GCATCCCGCG GCCCCAGCAC AAGGTGCGCG 1681 GTTACCGCTA CTTGGAGGAG GACAACTCGG ACGAGAGCGA CGCGGAGGGC GAGCATGGGG 1741 ACGGCGCGGA GGAGGAGGCG CCGCCCGCAG GGCCCAGGCC TGGCCCCGAG CCCGCTGGAC 1801 TCGGCCGCCG GCCCTGCCCG TACGAACAGG CGCAGGGGGG AGACGGGCCG GAGGAGCAGT 1861 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1921 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1981 TTTATATATC TTGTGGAAAG GACGACAAGA GCGCCTAAAG GCCGCTAACA CGCGTTAAGT 2041 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt