Transcript: Mouse NM_001161428.1

Mus musculus unc-93 homolog B1 (C. elegans) (Unc93b1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Unc93b1 (54445)
Length:
2003
CDS:
9..1277

Additional Resources:

NCBI RefSeq record:
NM_001161428.1
NBCI Gene record:
Unc93b1 (54445)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001161428.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000175263 CTTCTTTATCTACAGTGGCTT pLKO.1 1106 CDS 100% 2.640 2.112 N Unc93b1 n/a
2 TRCN0000175582 CAACTGATCCTGCACTATGAT pLKO.1 324 CDS 100% 5.625 3.938 N Unc93b1 n/a
3 TRCN0000175144 GATTTACTTCCTCAACAACTA pLKO.1 788 CDS 100% 4.950 3.465 N Unc93b1 n/a
4 TRCN0000173466 GCAGGACTTCATCTTCACCAT pLKO.1 1195 CDS 100% 2.640 1.848 N Unc93b1 n/a
5 TRCN0000194528 GCTGCCCATGATTTACTTCCT pLKO.1 779 CDS 100% 2.640 1.848 N Unc93b1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001161428.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04248 pDONR223 100% 56.8% 57.3% None (many diffs) n/a
2 ccsbBroad304_04248 pLX_304 0% 56.8% 57.3% V5 (many diffs) n/a
3 TRCN0000477552 CAAGAGCGCCTAAAGGCCGCTAAC pLX_317 17.1% 56.8% 57.3% V5 (many diffs) n/a
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