Construct: ORF TRCN0000477554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015127.1_s317c1
- Derived from:
- ccsbBroadEn_07204
- DNA Barcode:
- GACGTCATATTTTGGCCCCCAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAF15 (8148)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8148 | TAF15 | TATA-box binding protein as... | NM_139215.3 | 99.9% | 100% | 1524C>T |
| 2 | human | 8148 | TAF15 | TATA-box binding protein as... | NM_003487.4 | 99.4% | 99.4% | 175_176insGTCAGTCAG;1515C>T |
| 3 | human | 8148 | TAF15 | TATA-box binding protein as... | XM_024450959.1 | 54.5% | 54.5% | 0_1ins807;717C>T |
| 4 | mouse | 70439 | Taf15 | TATA-box binding protein as... | NM_027427.2 | 87.3% | 89.8% | (many diffs) |
| 5 | mouse | 70439 | Taf15 | TATA-box binding protein as... | XM_006534185.3 | 74.3% | 63% | (many diffs) |
| 6 | mouse | 70439 | Taf15 | TATA-box binding protein as... | XM_006534187.3 | 68.7% | 57.8% | (many diffs) |
| 7 | mouse | 70439 | Taf15 | TATA-box binding protein as... | XM_011249252.2 | 45.1% | 47.8% | (many diffs) |
| 8 | mouse | 70439 | Taf15 | TATA-box binding protein as... | XM_017314756.1 | 45.1% | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1845
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcggattct ggaagttacg gtcagtctgg gggtgagcag caaagttatt 121 ctacctatgg aaatccaggc agccaaggct atggacaagc atcacaaagc tattctggct 181 atgggcaaac gactgattcc tcttatggac agaactacag cggttactcc agttatggac 241 aaagtcagtc aggttattca cagtcctatg gtggttatga gaatcaaaag cagagctcat 301 atagccagca accatataat aaccagggac agcagcaaaa catggaatca tcaggaagcc 361 aaggtggaag agcaccttcc tatgaccagc cagactatgg tcaacaagat tcatatgacc 421 agcagtcagg ctatgatcaa catcaaggct catatgatga gcagtcaaat tatgatcagc 481 agcatgattc ctatagtcaa aaccagcagt cctatcattc acaaagggaa aactacagcc 541 accacacaca agatgaccgt cgtgatgtga gtaggtatgg agaagataat agaggatatg 601 gcgggtcaca gggaggaggt agagggcgtg ggggatatga caaggatgga agaggtccta 661 tgacaggatc aagtggtggt gaccgcggtg gcttcaaaaa ttttggtggt cacagggatt 721 atggacccag aacagatgct gattcagaat ctgataattc agataacaac acaatctttg 781 tgcaaggact tggggagggt gtgtctacag atcaagttgg ggagttcttt aaacaaatag 841 gaattatcaa gacaaataag aagaccggaa aaccaatgat aaatctttat acagacaagg 901 acacaggaaa gccaaagggg gaggcaacag tgtcatttga tgaccctcct tcagctaagg 961 cagccattga ctggtttgat ggaaaagaat tccatggcaa catcattaaa gtgtcctttg 1021 ccactagaag acctgaattc atgagaggag gtggaagtgg aggtgggcgg cgaggccgtg 1081 gaggatatag aggtcgtgga ggctttcaag ggagaggtgg agaccccaaa agtggggatt 1141 gggtttgccc taatccgtca tgcggaaata tgaactttgc tcgaaggaat tcctgcaatc 1201 agtgcaatga gcctagacca gaggactctc gtccctcagg aggagatttc cgggggagag 1261 gctacggtgg agagaggggc tacagaggtc gtgggggcag aggtggagac cgaggcggct 1321 atggtggaga cagaagtggg ggtggctatg gtggagacag aagcagcggt ggtggctaca 1381 gcggagatag aagtgggggc ggctatggtg gagacagaag tgggggtggc tatggtgggg 1441 acagaggcgg cggctatggt ggggacagag gaggcggcta tggaggagac cgaggaggtg 1501 gctatggagg agatcgaggt ggctatggag gagaccgagg tggaggctat ggtggagacc 1561 gaggaggcta tggaggagat cgaggaggtt atggaggaga tcgaggaggt tatggaggag 1621 atcgaggagg ctatggagga gacagaagcc gggggggcta tggaggagac cgtggtggtg 1681 gcagtggcta cggtggagac cgaagtggag gctatggagg agacaggagt ggtggcggct 1741 atggaggaga ccgaggtggg ggctacggag gagaccgagg tggctatgga ggcaaaatgg 1801 gaggaagaaa cgactacaga aatgatcagc gcaaCCGACC ATACTTGCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA GACGTCATAT TTTGGCCCCC AGCCACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt