Construct: ORF TRCN0000477589
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015888.1_s317c1
- Derived from:
- ccsbBroadEn_08854
- DNA Barcode:
- TCGAAATTATAATTCTGGCCCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT17 (64409)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477589
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64409 | GALNT17 | polypeptide N-acetylgalacto... | NM_022479.3 | 99.9% | 100% | 1566T>C |
| 2 | human | 64409 | GALNT17 | polypeptide N-acetylgalacto... | XM_011516467.3 | 83.8% | 83.6% | (many diffs) |
| 3 | human | 64409 | GALNT17 | polypeptide N-acetylgalacto... | XM_017012521.2 | 64.5% | 58.6% | (many diffs) |
| 4 | human | 64409 | GALNT17 | polypeptide N-acetylgalacto... | XM_011516469.3 | 54.2% | 53.5% | (many diffs) |
| 5 | mouse | 212996 | Wbscr17 | Williams-Beuren syndrome ch... | NM_145218.3 | 89.4% | 98.4% | (many diffs) |
| 6 | mouse | 212996 | Wbscr17 | Williams-Beuren syndrome ch... | XM_006504402.3 | 40.6% | 45.1% | (many diffs) |
| 7 | mouse | 212996 | Wbscr17 | Williams-Beuren syndrome ch... | XM_006504403.3 | 40.6% | 45.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1860
- ORF length:
- 1794
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcactgaga agagtcaaag tgctgttggt gttgaacttg atcgcggtag 121 ccggcttcgt gctcttcctg gccaagtgcc ggcccatcgc ggtgcgcagc ggagacgcct 181 tccacgagat ccggccgcgc gccgaggtgg ccaacctcag cgcgcacagc gccagcccca 241 tccaggatgc ggtcctgaag cgcctgtcgc tgctggagga catcgtgtac cggcagctga 301 atggcttatc caaatccctt gggctcattg aaggttatgg tgggcggggt aaagggggcc 361 ttccggctac tctttccccg gctgaagaag aaaaggctaa gggaccccat gagaagtatg 421 gctacaattc atacctcagt gaaaaaattt cactggaccg ttccattccg gattatcgtc 481 ccaccaagtg taaggagctc aagtactcca aggacctgcc ccagatatcc atcatattca 541 tcttcgtgaa cgaggccctg tcggtgatcc tgcggtccgt gcacagtgcc gtcaatcaca 601 cgcccacaca cctgctgaag gaaatcattc tggtggatga caacagcgac gaagaggagc 661 tgaaggtccc cctagaggag tatgtccaca aacgctaccc cgggctggtg aaggtggtaa 721 gaaatcagaa gagggaaggc ctgatccgcg ctcgcattga gggctggaag gtggctaccg 781 ggcaggtcac tggcttcttt gatgcccacg tggaattcac cgctggctgg gctgagccgg 841 ttctatcccg catccaggaa aaccggaagc gtgtgatcct cccctccatt gacaacatca 901 aacaggacaa ctttgaggtg cagcggtacg agaactcggc ccacgggtac agctgggagc 961 tgtggtgcat gtacatcagc cccccaaaag actggtggga cgccggagac ccttctctcc 1021 ccatcaggac cccagccatg ataggctgct cgttcgtggt caacaggaag ttcttcggtg 1081 aaattggtct tctggatcct ggcatggatg tatacggagg agaaaatatt gaactgggaa 1141 tcaaggtatg gctctgtggg ggcagcatgg aggtccttcc ttgctcacgg gtggcccaca 1201 ttgagcggaa gaagaagcca tataatagca acattggctt ctacaccaag aggaatgctc 1261 ttcgcgttgc tgaggtctgg atggacgatt acaagtctca tgtgtacata gcgtggaacc 1321 tgccgctgga gaatccggga attgacatcg gtgatgtcTC CGAAAGAAGA GCATTAAGGA 1381 AAAGTTTAAA GTGTAAGAAT TTCCAGTGGT ACCTGGACCA TGTTTACCCA GAAATGAGAA 1441 GATACAATAA TACCGTTGCT TACGGGGAGC TTCGCAACAA CAAGGCAAAA GACGTCTGCT 1501 TGGACCAGGG GCCGCTGGAG AACCACACAG CAATATTGTA TCCGTGCCAT GGCTGGGGAC 1561 CACAGCTTGC CCGCTACACC AAGGAAGGCT TCCTGCACTT GGGTGCCCTG GGGACCACCA 1621 CACTCCTCCC CGACACCCGC TGCCTGGTGG ACAACTCCAA GAGTCGGCTG CCCCAGCTCC 1681 TGGACTGCGA CAAGGTCAAG AGCAGCCTGT ACAAGCGCTG GAACTTCATC CAGAATGGAG 1741 CCATCATGAA CAAGGGCACG GGACGCTGCC TGGAGGTGGA GAACCGGGGC CTGGCTGGCA 1801 TCGACCTCAT CCTCCGCAGC TGCACAGGTC AGAGGTGGAC CATTAAGAAC TCCATCAAGT 1861 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1921 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1981 TTTATATATC TTGTGGAAAG GACGATCGAA ATTATAATTC TGGCCCTCAA CGCGTTAAGT 2041 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt