Construct: ORF TRCN0000477598
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005830.1_s317c1
- Derived from:
- ccsbBroadEn_09019
- DNA Barcode:
- GTGCCGTGCCTGGACTGCATAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ORAI2 (80228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477598
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80228 | ORAI2 | ORAI calcium release-activa... | NM_001126340.3 | 99.8% | 100% | 57C>A |
| 2 | human | 80228 | ORAI2 | ORAI calcium release-activa... | NM_001271818.1 | 99.8% | 100% | 57C>A |
| 3 | human | 80228 | ORAI2 | ORAI calcium release-activa... | NM_032831.4 | 99.8% | 100% | 57C>A |
| 4 | human | 80228 | ORAI2 | ORAI calcium release-activa... | NM_001271819.2 | 69.6% | 69.6% | 0_1ins231 |
| 5 | mouse | 269717 | Orai2 | ORAI calcium release-activa... | NM_178751.3 | 88.7% | 95.2% | (many diffs) |
| 6 | mouse | 269717 | Orai2 | ORAI calcium release-activa... | XM_006504430.3 | 88.7% | 95.2% | (many diffs) |
| 7 | mouse | 269717 | Orai2 | ORAI calcium release-activa... | XM_006504431.3 | 88.7% | 95.2% | (many diffs) |
| 8 | mouse | 269717 | Orai2 | ORAI calcium release-activa... | XM_006504429.3 | 84% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 828
- ORF length:
- 762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgctgagctt aacgtgccta tcgacccctc tgctcctgcc tgccctgagc 121 caggccataa gggcatggat taccgggact gggtccgccg cagctacctg gaactggtca 181 cctctaacca ccactcggta caggccctgt cgtggcggaa gctctacctg agcagggcca 241 agctgaaggc ctccagcagg acctccgccc tcctctccgg ctttgccatg gtggccatgg 301 tggaggtgca gctggagacg cagtaccagt acccgcggcc gctgctgatt gccttcagcg 361 cctgcaccac ggtgctggtg gccgtgcacc tgttcgccct cctcatcagc acctgcatcc 421 tgcccaatgt ggaggccgtg agcaacatcc acaacctgaa ctccatcagc gagtccccgc 481 atgagcgcat gcaccccTAC ATCGAGCTGG CCTGGGGCTT CTCCACCGTG CTTGGCATCC 541 TACTCTTCCT GGCCGAGGTG GTGCTGCTCT GCTGGATCAA GTTCCTCCCC GTGGATGCCC 601 GGCGCCAGCC TGGCCCCCCA CCTGGCCCTG GGAGTCACAC GGGCTGGCAG GCCGCCCTGG 661 TGTCCACCAT CATCATGGTG CCCGTGGGCC TCATCTTCGT GGTCTTCACC ATCCACTTCT 721 ACCGCTCCCT GGTGCGCCAC AAAACGGAGC GCCACAACCG CGAGATCGAG GAGCTCCACA 781 AGCTCAAGGT CCAGCTGGAC GGGCATGAGC GCAGCCTGCA GGTCTTGTGC CCAACTTTCT 841 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 901 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 961 GTGGAAAGGA CGAGTGCCGT GCCTGGACTG CATAACAACG CGTTAAGTCg acaatcaacc 1021 tctggattac aaaatttgtg aaagatt