Transcript: Human NM_001271819.2

Homo sapiens ORAI calcium release-activated calcium modulator 2 (ORAI2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
ORAI2 (80228)
Length:
10509
CDS:
203..736

Additional Resources:

NCBI RefSeq record:
NM_001271819.2
NBCI Gene record:
ORAI2 (80228)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001271819.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000160083 CCTCTTGTCTTAATACCATAA pLKO.1 850 3UTR 100% 10.800 7.560 N ORAI2 n/a
2 TRCN0000164849 CTGGATGACTTCTCCTGAGAT pLKO.1 874 3UTR 100% 4.950 3.465 N ORAI2 n/a
3 TRCN0000162008 GATTTGTCAGATGCAGACATT pLKO.1 793 3UTR 100% 4.950 3.465 N ORAI2 n/a
4 TRCN0000164122 CTGAGATAGAACCGTTTGGTT pLKO.1 888 3UTR 100% 3.000 2.100 N ORAI2 n/a
5 TRCN0000166579 CATCTTCGTGGTCTTCACCAT pLKO.1 598 CDS 100% 2.640 1.848 N ORAI2 n/a
6 TRCN0000162862 GCTTGTACAACCAAACACCTT pLKO.1 2018 3UTR 100% 2.640 1.848 N ORAI2 n/a
7 TRCN0000126316 CGTGGTCTTCACCATCCACTT pLKO.1 604 CDS 100% 4.050 2.430 N Orai2 n/a
8 TRCN0000164937 GAGCAACATCCACAACCTGAA pLKO.1 346 CDS 100% 4.050 2.430 N ORAI2 n/a
9 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 2207 3UTR 100% 4.950 2.475 Y ORAI2 n/a
10 TRCN0000084008 CGCCTATAATCCCAGCACTTT pLKO.1 3545 3UTR 100% 4.950 2.475 Y NPHS1 n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 2129 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 7633 3UTR 100% 4.950 2.475 Y LOC387873 n/a
13 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 2168 3UTR 100% 4.050 2.025 Y P3H4 n/a
14 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 2168 3UTR 100% 4.050 2.025 Y ORAI2 n/a
15 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 2168 3UTR 100% 4.050 2.025 Y P3H4 n/a
16 TRCN0000126318 GTGTCCACCATCATCATGGTA pLKO.1 566 CDS 100% 0.300 0.180 N Orai2 n/a
17 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2130 3UTR 100% 13.200 6.600 Y LIAS n/a
18 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 6070 3UTR 100% 5.625 2.813 Y KLHL30 n/a
19 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 2204 3UTR 100% 4.950 2.475 Y LOC339059 n/a
20 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 6070 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001271819.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09019 pDONR223 100% 69.6% 69.6% None 0_1ins231 n/a
2 ccsbBroad304_09019 pLX_304 0% 69.6% 69.6% V5 0_1ins231 n/a
3 TRCN0000477598 GTGCCGTGCCTGGACTGCATAACA pLX_317 43.6% 69.6% 69.6% V5 0_1ins231 n/a
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