Transcript: Mouse XM_006504431.3

PREDICTED: Mus musculus ORAI calcium release-activated calcium modulator 2 (Orai2), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Orai2 (269717)
Length:
3936
CDS:
215..967

Additional Resources:

NCBI RefSeq record:
XM_006504431.3
NBCI Gene record:
Orai2 (269717)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006504431.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256394 CCGCATGAATAAGGGTCTAAA pLKO_005 1165 3UTR 100% 13.200 18.480 N Orai2-ps n/a
2 TRCN0000126314 CCCGCATGAATAAGGGTCTAA pLKO.1 1164 3UTR 100% 4.950 6.930 N Orai2 n/a
3 TRCN0000256395 CTGGAACTCGTCACGTCTAAC pLKO_005 317 CDS 100% 10.800 7.560 N Orai2-ps n/a
4 TRCN0000256393 TGGTAGCCGTGCATCTGTTTG pLKO_005 525 CDS 100% 10.800 7.560 N Orai2-ps n/a
5 TRCN0000265781 CCACAACCGTGAGATCGAAGA pLKO_005 889 CDS 100% 4.050 2.835 N Orai2-ps n/a
6 TRCN0000126316 CGTGGTCTTCACCATCCACTT pLKO.1 835 CDS 100% 4.050 2.835 N Orai2 n/a
7 TRCN0000126317 CGGCCACAAGGGCATGGATTA pLKO.1 271 CDS 100% 3.600 2.520 N Orai2 n/a
8 TRCN0000166579 CATCTTCGTGGTCTTCACCAT pLKO.1 829 CDS 100% 2.640 1.848 N ORAI2 n/a
9 TRCN0000165046 GAGCAACATCCACAACCTCAA pLKO.1 589 CDS 100% 4.050 2.430 N ORAI3 n/a
10 TRCN0000126315 GCATCTGTTTGCTCTGCTCAT pLKO.1 535 CDS 100% 4.050 2.430 N Orai2 n/a
11 TRCN0000265788 GGGTCTCATCTTCGTGGTCTT pLKO_005 823 CDS 100% 4.050 2.430 N Orai2-ps n/a
12 TRCN0000126318 GTGTCCACCATCATCATGGTA pLKO.1 797 CDS 100% 0.300 0.180 N Orai2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006504431.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09019 pDONR223 100% 88.7% 95.2% None (many diffs) n/a
2 ccsbBroad304_09019 pLX_304 0% 88.7% 95.2% V5 (many diffs) n/a
3 TRCN0000477598 GTGCCGTGCCTGGACTGCATAACA pLX_317 43.6% 88.7% 95.2% V5 (many diffs) n/a
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