Construct: ORF TRCN0000477689
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018316.1_s317c1
- Derived from:
- ccsbBroadEn_08023
- DNA Barcode:
- GGCCTGACAATTTGTCATGTACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGO1 (26523)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477689
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26523 | AGO1 | argonaute RISC component 1 | NM_012199.5 | 99.9% | 99.8% | 724G>A |
| 2 | human | 26523 | AGO1 | argonaute RISC component 1 | XM_011541236.2 | 99.6% | 99.5% | 724G>A;1262_1270delGGGTGAGCA |
| 3 | human | 26523 | AGO1 | argonaute RISC component 1 | XM_017001017.1 | 98.1% | 98% | 724G>A;2262_2309del |
| 4 | human | 26523 | AGO1 | argonaute RISC component 1 | XM_017001016.1 | 97.7% | 97.7% | 724G>A;1262_1270delGGGTGAGCA;2271_2318del |
| 5 | human | 26523 | AGO1 | argonaute RISC component 1 | NM_001317122.1 | 96.1% | 99.8% | 724G>A;2572_2673del |
| 6 | human | 26523 | AGO1 | argonaute RISC component 1 | NM_001317123.1 | 91.2% | 91.1% | 0_1ins225;499G>A |
| 7 | human | 26523 | AGO1 | argonaute RISC component 1 | XM_017001019.1 | 81.5% | 80.3% | (many diffs) |
| 8 | human | 26523 | AGO1 | argonaute RISC component 1 | XM_017001018.1 | 79.7% | 78.6% | (many diffs) |
| 9 | mouse | 236511 | Ago1 | argonaute RISC catalytic su... | NM_153403.3 | 93.4% | 99.8% | (many diffs) |
| 10 | mouse | 236511 | Ago1 | argonaute RISC catalytic su... | XM_011240520.2 | 93.1% | 99.5% | (many diffs) |
| 11 | mouse | 236511 | Ago1 | argonaute RISC catalytic su... | NM_001317173.1 | 89.4% | 99.8% | (many diffs) |
| 12 | mouse | 236511 | Ago1 | argonaute RISC catalytic su... | NM_001317174.1 | 76.4% | 80.3% | (many diffs) |
| 13 | mouse | 236511 | Ago1 | argonaute RISC catalytic su... | XM_011240521.1 | 76.1% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2637
- ORF length:
- 2571
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agcgggaccc tcgggagcag ctgcgggcgc ttacctgccc cccctgcagc 121 aggtgttcca ggcacctcgc cggcctggca ttggcactgt ggggaaacca atcaagctcc 181 tggccaatta ctttgaggtg gacatcccta agatcgacgt gtaccactac gaggtggaca 241 tcaagccgga taagtgtccc cgtagagtca accgggaagt ggtggaatac atggtccagc 301 atttcaagcc tcagatcttt ggtgatcgca agcctgtgta tgatggaaag aagaacattt 361 acactgtcac agcactgccc attggcaacg aacgggtcga ctttgaggtg acaatccctg 421 gggaagggaa ggatcgaatc tttaaggtct ccatcaagtg gctagccatt gtgagctggc 481 gaatgctgca tgaggccctg gtcagcggcc agatccctgt tcccttggag tctgtgcaag 541 ccctggatgt ggccatgagg cacctggcat ccatgaggta cacccctgtg ggccgctcct 601 tcttctcacc gcctgagggc tactaccacc cgctgggggg tgggcgcgag gtctggttcg 661 gctttcacca gtctgtgcgc cctgccatgt ggaagatgat gctcaacatt gatgtctcag 721 ccactgcctt ttataaggca cagccagtga ttgagttcat gtgtgaggtg ctggacatca 781 ggaacataaa tgagcagccc aagcccctca cggactctca gcgcgttcgc ttcaccaagg 841 agatcaaggg cctgaaggtg gaagtcaccc actgtggaca gatgaagagg aagtaccgcg 901 tgtgtaatgt tacccgtcgc cctgctagcc atcagacatt ccccttacag ctggagagtg 961 gacagactgt ggagtgcaca gtggcacagt atttcaagca gaaatataac cttcagctca 1021 agtatcccca tctgccctgc ctacaagttg gccaggaaca aaagcatacc taccttcccc 1081 tagaggtctg taacattgtg gctgggcagc gctgtattaa aaagctgacc gacaaccaga 1141 cctcgaccat gataaaggcc acagctagat ccgctccaga cagacaggag gagatcagtc 1201 gcctgatgaa gaatgccagc tacaacttag atccctacat ccaggaattt gggatcaaag 1261 tgaaggatga catgacggag gtgacagggc gagtgctgcc ggcgcccatc ttgcagtacg 1321 gcggccggaa ccgggccatt gccacaccca atcagggtgt ctgggacatg cgggggaaac 1381 agttctacaa tgggattgag atcaaagtct gggccatcgc ctgcttcgca ccccaaaaac 1441 agtgtcgaga agaggtgctc aagaacttca cagaccagct gcggaagatt tccaaggatg 1501 cggggatgcc tatccagggt caaccttgtt tctgcaaata tgcacagggg gcagacagcg 1561 tggagcctat gttccggcat ctcaagaaca cctactcagg gctgcagctc attattgtca 1621 tcctgccagg gaagacgccg gtgtatgctg aggtgaaacg tgtcggagat acactcttgg 1681 gaatggctac gcagtgtgtg caggtgaaga acgtggtcaa gacctcacct cagactctgt 1741 ccaacctctg cctcaagatc aatgtcaaac ttggtggcat taacaacatc ctagtcccac 1801 accagcgctc tgccgttttt caacagccag tgatattcct gggagcagat gttacacacc 1861 ccccagcagg ggatgggaaa aaaccttcta tcacagcagt ggtaggcagt atggatgccc 1921 accccagccg atactgtgct actgtgcggg tacagcgacc acggcaagag atcattgaag 1981 acttgtccta catggtgcgt gagctcctca tccaattcta caagtccacc cgtttcaagc 2041 ctacccgcat catcttctac cgagatgggg tgcctgaagg ccagctaccc cagatactcc 2101 actatgagct actggccatt cgtgatgcct GCATCAAACT GGAAAAGGAC TACCAGCCTG 2161 GGATCACTTA TATTGTGGTG CAGAAACGCC ATCACACCCG CCTTTTCTGT GCTGACAAGA 2221 ATGAGCGAAT TGGGAAGAGT GGTAACATCC CAGCTGGGAC CACAGTGGAC ACCAACATCA 2281 CCCACCCATT TGAGTTTGAC TTCTATCTGT GCAGCCACGC AGGCATCCAG GGCACCAGCC 2341 GACCATCCCA TTACTATGTT CTTTGGGATG ACAACCGTTT CACAGCAGAT GAGCTCCAGA 2401 TCCTGACGTA CCAGCTGTGC CACACTTACG TACGATGCAC ACGCTCTGTC TCTATCCCAG 2461 CACCTGCCTA CTATGCCCGC CTGGTGGCTT TCCGGGCACG ATACCACCTG GTGGACAAGG 2521 AGCATGACAG TGGAGAGGGG AGCCACATAT CGGGGCAGAG CAATGGGCGG GACCCCCAGG 2581 CCCTGGCCAA AGCCGTGCAG GTTCACCAGG ATACTCTGCG CACCATGTAC TTCGCTTGCC 2641 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2701 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2761 ATATATCTTG TGGAAAGGAC GAGGCCTGAC AATTTGTCAT GTACTCACGC GTTAAGTCga 2821 caatcaacct ctggattaca aaatttgtga aagatt