Transcript: Mouse NM_001317174.1

Mus musculus argonaute RISC catalytic subunit 1 (Ago1), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Ago1 (236511)
Length:
7337
CDS:
698..2818

Additional Resources:

NCBI RefSeq record:
NM_001317174.1
NBCI Gene record:
Ago1 (236511)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001317174.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000009629 CCATTTGAGTTCGACTTCTAT pLKO.1 2465 CDS 100% 5.625 7.875 N Ago1 n/a
2 TRCN0000009625 CGCGGGAAACAGTTCTACAAT pLKO.1 1550 CDS 100% 5.625 7.875 N Ago1 n/a
3 TRCN0000009628 CCTAAGATTGACGTTTACCAT pLKO.1 304 5UTR 100% 3.000 2.400 N Ago1 n/a
4 TRCN0000435961 ATTGAAGACTTGTCCTATATG pLKO_005 2153 CDS 100% 13.200 9.240 N Ago1 n/a
5 TRCN0000415676 ACAATGGGATTGAGATCAAAG pLKO_005 1566 CDS 100% 10.800 7.560 N AGO1 n/a
6 TRCN0000413322 TTGAAGGCAGAACGCTGTTAC pLKO_005 2815 CDS 100% 10.800 7.560 N Ago1 n/a
7 TRCN0000009627 CCAGCATTTCAAACCGCAGAT pLKO.1 393 5UTR 100% 4.050 2.835 N Ago1 n/a
8 TRCN0000009626 GCTGACAAGAATGAGCGGATT pLKO.1 2390 CDS 100% 4.050 2.835 N Ago1 n/a
9 TRCN0000007861 GCACAGTATTTCAAGCAGAAA pLKO.1 1163 CDS 100% 4.950 2.970 N AGO1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001317174.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08023 pDONR223 100% 76.4% 80.3% None (many diffs) n/a
2 ccsbBroad304_08023 pLX_304 0% 76.4% 80.3% V5 (many diffs) n/a
3 TRCN0000477689 GGCCTGACAATTTGTCATGTACTC pLX_317 19.9% 76.4% 80.3% V5 (many diffs) n/a
4 ccsbBroadEn_11852 pDONR223 100% 43% 47.8% None (many diffs) n/a
5 ccsbBroad304_11852 pLX_304 0% 43% 47.8% V5 (many diffs) n/a
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