Construct: ORF TRCN0000477739
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007597.1_s317c1
- Derived from:
- ccsbBroadEn_08875
- DNA Barcode:
- ATCGCGTGGAGATTTTCCGCGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARCHF7 (64844)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477739
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64844 | MARCHF7 | membrane associated ring-CH... | NM_001282805.2 | 99.9% | 100% | 651C>T;1467T>C |
| 2 | human | 64844 | MARCHF7 | membrane associated ring-CH... | NM_022826.4 | 99.9% | 100% | 651C>T;1467T>C |
| 3 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XM_005246773.2 | 99.9% | 100% | 651C>T;1467T>C |
| 4 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XM_005246774.1 | 97.1% | 94.3% | (many diffs) |
| 5 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XM_017004718.2 | 97.1% | 94.3% | (many diffs) |
| 6 | human | 64844 | MARCHF7 | membrane associated ring-CH... | NM_001282807.2 | 93.6% | 91.9% | (many diffs) |
| 7 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XM_017004719.1 | 91.9% | 92% | 346_347ins168;483C>T;1299T>C |
| 8 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XM_017004720.1 | 91.9% | 92% | 346_347ins168;483C>T;1299T>C |
| 9 | human | 64844 | MARCHF7 | membrane associated ring-CH... | NM_001282806.1 | 89.2% | 86.4% | (many diffs) |
| 10 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XM_017004721.1 | 89.2% | 86.4% | (many diffs) |
| 11 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XR_001738901.2 | 60.2% | (many diffs) | |
| 12 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XR_427106.1 | 59.3% | (many diffs) | |
| 13 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XR_922987.2 | 57.9% | (many diffs) | |
| 14 | human | 64844 | MARCHF7 | membrane associated ring-CH... | XR_001738902.1 | 54.6% | (many diffs) | |
| 15 | mouse | 57438 | March7 | membrane-associated ring fi... | NM_020575.2 | 85.4% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2181
- ORF length:
- 2112
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtctaaa ccttcaagga ttccaagaag aatttctgtt caaccttcca 121 gctccttaag tgctaggatg atgtctggaa gcagaggaag tagtttaaat gatacctatc 181 actcaagaga ctcttcattt agattggatt ctgaatatca gtctacatca gcatcagcat 241 ctgcgtcacc atttcaatct gcatggtata gtgaatctga gataactcag ggagcacgct 301 caagatcgca gaaccagcaa cgggatcatg attcaaaaag acctaaactt tcctgtacaa 361 actgtactac ctcagctggg agaaatgttg gaaatggttt aaacacatta tcagattcat 421 cttggaggca tagtcaagtt cctagatctt catcaatggt acttggatca tttggaacag 481 acttaatgag agagaggaga gatttggaga gaagaacaga ttcctctatt agtaatctta 541 tggattatag tcaccgaagt ggtgatttca caacttcatc atatgttcaa gacagagttc 601 cttcatattc acaaggagca agaccaaaag aaaactcaat gagcacttta cagttgaata 661 catcatccac aaaccaccaa ttgccttctg aacatcagac catactaagt tctagggatt 721 ccagaaattc tttaagatca aatttttctt caagagaatc agaatcttcc cgaagcaata 781 cgcagcctgg attttcttac agttcaagta gagatgaagc cccaatcata agcaattcag 841 aaagggttgt ttcatctcaa agaccatttc aagaatcttc tgacaatgaa ggtaggcgga 901 caacgaggag attgctgtca cgcatagctt ctagcatgtc atctactttt ttttcacgaa 961 gatctagtca ggattccttg aatacaagat cattgaattc tgaaaattct tacgtttctc 1021 caagaatctt gacagcttca cagtcccgta gtaatgtacc atcagcttct gaagttcccg 1081 ataatagggc atctgaagct tctcagggat ttcgatttct taggcgaaga tggggtttgt 1141 catctcttag ccacaatcat agctctgagt cagattcaga aaattttaac caagaatctg 1201 aaggtagaaa tacaggacca tggttatctt cctcacttag aaatagatgc acacctttgt 1261 tctctagaag gaggcgagag ggaagagatg aatcttcaag gatacctacc tctgatacat 1321 catctagatc tcatattttt agaagagaat caaatgaagt ggttcacctt gaagcacaga 1381 atgatcctct tggagctgct gccaacagac cacaagcatc tgcagcatca agcagtgcca 1441 caacaggtgg ctctacatca gattcggctc aaggtggaag aaatacagga atatcaggga 1501 ttcttcctgg ttccttattc cggtttgcag tccccccagc acttgggagt aatttgaccg 1561 acaatgtcat gatcacagta gatattattc cttcaggttg gaattcagct gatggtaaaa 1621 gtgataaaac taaaagtgcg ccttcaagag atccagaaag attgcagaaa ataaaagaga 1681 gcctcctttt agaggactca gaagaagaag aaggtgactt atgtagaaTT TGTCAAATGG 1741 CAGCTGCATC ATCATCTAAT TTGCTGATAG AGCCATGCAA GTGCACAGGA AGTTTGCAGT 1801 ATGTCCACCA AGACTGTATG AAAAAGTGGT TACAGGCCAA AATTAACTCT GGTTCTTCAT 1861 TAGAAGCTGT AACCACCTGT GAACTATGTA AAGAGAAGTT GGAGCTTAAC CTGGAGGATT 1921 TTGATATTCA TGAACTACAT AGAGCTCATG CAAATGAACA AGCTGAGTAT GAGTTTATCA 1981 GCTCTGGTCT CTACCTAGTG GTGTTATTGC ACTTGTGCGA ACAAAGCTTT TCTGATATGA 2041 TGGGAAATAC AAATGAACCA AGCACACGTG TCCGATTTAT TAACCTTGCA AGAACTCTTC 2101 AGGCACATAT GGAAGATCTC GAAACTTCAG AGGATGATTC CGAAGAAGAC GGAGACCATA 2161 ACAGGACATT TGATATTGCC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2221 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2281 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATCG CGTGGAGATT 2341 TTCCGCGTAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt