Construct: ORF TRCN0000477741
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000194.1_s317c1
- Derived from:
- ccsbBroadEn_03540
- DNA Barcode:
- ATCGTTATCCTTCACCGTTCTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MSL2 (55167)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477741
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55167 | MSL2 | MSL complex subunit 2 | NM_018133.4 | 100% | 100% | |
| 2 | human | 55167 | MSL2 | MSL complex subunit 2 | NM_001145417.2 | 87.1% | 87.1% | 0_1ins222 |
| 3 | human | 55167 | MSL2 | MSL complex subunit 2 | XM_005247571.3 | 87.1% | 87.1% | 0_1ins222 |
| 4 | human | 55167 | MSL2 | MSL complex subunit 2 | XM_006713684.3 | 87.1% | 87.1% | 0_1ins222 |
| 5 | human | 55167 | MSL2 | MSL complex subunit 2 | XM_011512949.2 | 87.1% | 87.1% | 0_1ins222 |
| 6 | mouse | 77853 | Msl2 | male-specific lethal 2 homo... | NM_001100451.2 | 95.3% | 98.9% | (many diffs) |
| 7 | mouse | 77853 | Msl2 | male-specific lethal 2 homo... | XM_017313694.1 | 82.7% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1800
- ORF length:
- 1731
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaccccgtg aatgctactg ctctctacat ttccgcgagc cgcctagtgc 121 tcaactacga ccccggagac cccaaggcgt ttactgagat taacaggctc ttgccttact 181 tccgacagtc cctttcgtgc tgtgtttgcg gacatttgct acaagatcct attgcaccca 241 ccaactccac ctgccaacat tatgtctgca aaacttgtaa aggcaagaaa atgatgatga 301 aaccttcctg tagctggtgc aaagactatg agcagtttga ggaaaacaag cagttaagca 361 tcctagtgaa ctgctacaaa aaactatgcg agtatataac acagactaca ctggcacggg 421 atataataga agcagttgac tgttcttctg atattttggc tttgcttaat gatggatcat 481 tgttttgtga ggagacagaa aaaccctcag attcatcctt tactttgtgt ttgacacatt 541 cccctttacc ttcaacctca gaacccacaa ctgatcctca agctagttta tctccaatgt 601 ctgaaagcac cctcagcatt gctattggca gttctgttat caatggtttg cctacttata 661 atgggctttc aatagataga tttggtataa atattccttc acctgaacat tcaaatacga 721 ttgacgtatg taatactgtt gacataaaaa ctgaggatct gtctgacagc ctgccacccg 781 tttgtgacac agtagccact gacttatgtt ccacaggcat tgatatctgc agtttcagtg 841 aagatataaa acctggagac tctctgttac tgagtgttga ggaagtactc cgcagcttag 901 aaactgtttc aaatacagag gtctgttgcc ctaatttgca gccgaacttg gaagccactg 961 tatccaatgg accttttctg cagctttctt cccagtctct tagccataat gtttttatgt 1021 ccaccagtcc tgcacttcat gggttatcat gtacagcagc aactccgaag atagcaaaat 1081 tgaatagaaa acgatccaga tcagagagtg acagtgagaa agttcagcca cttccaattt 1141 ctaccattat ccgaggccca acactggggg catctgctcc tgtgacagtg aaacgggaga 1201 gcaaaatttc tcttcaacct atagcaactg ttcccaatgg aggcacaaca cctaaaatca 1261 gcaaaactgt acttttatct actaaaagca tgaaaaagag tcatgaacat ggatccaaga 1321 aatctcactc taaaaccaag ccaggtattc ttaaaaaaga caaagcagta aaggaaaaga 1381 ttcctagtca tcattttatg ccaggaagtc ctaccaagac tgtgtacaaa aaaccccagg 1441 aaaagaaagg gtgtaaatgt gggcgtgcta ctcaaaatCC AAGTGTTCTT ACATGCCGAG 1501 GCCAACGCTG CCCTTGCTAC TCTAACCGCA AAGCCTGCTT AGATTGTATA TGTCGTGGCT 1561 GCCAAAACTC CTATATGGCC AATGGGGAGA AGAAGCTGGA GGCATTTGCC GTGCCAGAAA 1621 AGGCCTTGGA GCAGACCAGG CTCACTTTGG GCATTAACGT GACTAGCATT GCTGTGCGTA 1681 ACGCTAGTAC CAGCACCAGT GTAATAAATG TCACAGGGTC CCCAGTAACG ACGTTTTTAG 1741 CTGCCAGTAC ACATGATGAT AAAAGTTTGG ATGAAGCTAT AGACATGAGA TTCGACTGTT 1801 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1861 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1921 TTTATATATC TTGTGGAAAG GACGAATCGT TATCCTTCAC CGTTCTGTTA CGCGTTAAGT 1981 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt