Construct: ORF TRCN0000477792
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007996.1_s317c1
- Derived from:
- ccsbBroadEn_10723
- DNA Barcode:
- CAGTAGATAGTGAGCTCATATAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDH5 (1003)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477792
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1003 | CDH5 | cadherin 5 | NM_001795.5 | 85.2% | 85% | 1137_1481del;1550T>C;1805T>C |
| 2 | human | 1003 | CDH5 | cadherin 5 | XM_011522801.2 | 84.2% | 84.1% | (many diffs) |
| 3 | human | 1003 | CDH5 | cadherin 5 | XM_024450133.1 | 84.2% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2076
- ORF length:
- 2007
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagaggctc atgatgctcc tcgccacatc gggcgcctgc ctgggcctgc 121 tggcagtggc agcagtggca gcagcaggtg ctaaccctgc ccaacgggac acccacagcc 181 tgctgcccac ccaccggcgc caaaagagag attggatttg gaaccagatg cacattgatg 241 aagagaaaaa cacctcactt ccccatcatg taggcaagat caagtcaagc gtgagtcgca 301 agaatgccaa gtacctgctc aaaggagaat atgtgggcaa ggtcttccgg gtcgatgcag 361 agacaggaga cgtgttcgcc attgagaggc tggaccggga gaatatctca gagtaccacc 421 tcactgctgt cattgtggac aaggacactg gcgaaaacct ggagactcct tccagcttca 481 ccatcaaagt tcatgacgtg aacgacaact ggcctgtgtt cacgcatcgg ttgttcaatg 541 cgtccgtgcc tgagtcgtcg gctgtgggga cctcagtcat ctctgtgaca gcagtggatg 601 cagacgaccc cactgtggga gaccacgcct ctgtcatgta ccaaatcctg aaggggaaag 661 agtattttgc catcgataat tctggacgta ttatcacaat aacgaaaagc ttggaccgag 721 agaagcaggc caggtatgag atcgtggtgg aagcgcgaga tgcccagggc ctccgggggg 781 actcgggcac ggccaccgtg ctggtcactc tgcaagacat caatgacaac ttccccttct 841 tcacccagac caagtacaca tttgtcgtgc ctgaagacac ccgtgtgggc acctctgtgg 901 gctctctgtt tgttgaggac ccagatgagc cccagaaccg gatgaccaag tacagcatct 961 tgcggggcga ctaccaggac gctttcacca ttgagacaaa ccccgcccac aacgagggca 1021 tcatcaagcc catgaagcct ctggattatg aatacatcca gcaatacagc ttcatcgtcg 1081 aggccacaga ccccaccatc gacctccgat acatgagccc tcccgcggga aacagagccc 1141 aggtcattat caacatcaca gatgtggacg agccccccat tttccagcag cctttctacc 1201 acttccagct ggtcctgcag atctccgcaa tagacaagga cataacacca cgaaacgtga 1261 agttcaaatt caccttgaat actgagaaca actttaccct cacggataat cacgataaca 1321 cggccaacat cacagtcaag tatgggcagt ttgaccggga gcataccaag gtccacttcc 1381 tacccgtggt catctcagac aatgggatgc caagtcgcac gggcaccagc acgctgaccg 1441 tggccgtgtg caagtgcaac gagcagggcg agttcacctt ctgcgaggat atggccgccc 1501 aggtgggcgt gagcatccag gcagtggcag ccatcttact ctgcatcctc accatcacag 1561 tgatcaccct gctcatcttc ctgcggcggc ggctccggaa gcaggcccgc gcgcacggca 1621 agagcgtgcc ggagatccac gagcagctgg tcacctacga cgaggagggc ggcggcgaga 1681 tggacaccac cagctacgat gtgtcggtgc tcaactcggT GCGCCGCGGC GGGGCCAAGC 1741 CCCCGCGGCC CGCGCTGGAC GCCCGGCCTT CCCTCTATGC GCAGGTGCAG AAGCCACCGA 1801 GGCACGCGCC TGGGGCACAC GGAGGGCCCG GGGAGATGGC AGCCATGATC GAGGTGAAGA 1861 AGGACGAGGC GGACCACGAC GGCGACGGCC CCCCCTACGA CACGCTGCAC ATCTACGGCT 1921 ACGAGGGCTC CGAGTCCATA GCCGAGTCCC TCAGCTCCCT GGGCACCGAC TCATCCGACT 1981 CTGACGTGGA TTACGACTTC CTTAACGACT GGGGACCCAG GTTTAAGATG CTGGCTGAGC 2041 TGTACGGCTC GGACCCCCGG GAGGAGCTGC TGTATTTGCC AACTTTCTTG TACAAAGTGG 2101 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2161 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2221 ACAGTAGATA GTGAGCTCAT ATAACACGCG TTAAGTCgac aatcaacctc tggattacaa 2281 aatttgtgaa agatt