Construct: ORF TRCN0000477827
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001151.1_s317c1
- Derived from:
- ccsbBroadEn_01980
- DNA Barcode:
- CTCACCGCCTCGTGATCGCTTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNK5 (8645)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477827
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8645 | KCNK5 | potassium two pore domain c... | NM_003740.4 | 100% | 100% | |
| 2 | human | 8645 | KCNK5 | potassium two pore domain c... | XM_005249456.1 | 99.3% | 99.3% | 456_457insGTGAGTCTG |
| 3 | human | 8645 | KCNK5 | potassium two pore domain c... | XM_006715235.2 | 63.5% | 63.5% | 0_1ins546 |
| 4 | mouse | 16529 | Kcnk5 | potassium channel, subfamil... | NM_021542.4 | 84.3% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1563
- ORF length:
- 1497
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggaccggggc cctctgctca cctcggccat catcttctac ctggccatcg 121 gggcggcgat cttcgaagtg ctggaggagc cacactggaa ggaggccaag aaaaactact 181 acacacagaa gctgcatctg ctcaaggagt tcccgtgcct gggtcaggag ggcctggaca 241 agatcctaga ggtggtatct gatgctgcag gacagggtgt ggccatcaca gggaaccaga 301 ccttcaacaa ctggaactgg cccaatgcaa tgatttttgc agcgaccgtc attaccacca 361 ttggatatgg caatgtggct cccaagaccc ccgccggtcg cctcttctgt gttttctatg 421 gtctcttcgg ggtgccgctc tgcctgacgt ggatcagtgc cctgggcaag ttcttcgggg 481 gacgtgccaa gagactaggg cagttcctta ccaagagagg tgtgagtctg cggaaggcgc 541 agatcacgtg cacagtcatc ttcatcgtgt ggggcgtcct agtccacctg gtgatcccac 601 ccttcgtatt catggtgact gaggggtgga actacatcga gggcctctac tactccttca 661 tcaccatctc caccatcggc ttcggtgact ttgtggccgg tgtgaacccc agcgccaact 721 accacgccct gtaccgctac ttcgtggagc tctggatcta cttggggctg gcctggctgt 781 ccctttttgt caactggaag gtgagcatgt ttgtggaagt ccacaaagcc attaagaagc 841 ggcggcggcg acggaaggag tcctttgaga gctccccaca ctcccggaag gccctgcagg 901 tgaaggggag cacagcctcc aaggacgtca acatcttcag ctttctttcc aagaaggaag 961 agacctacaa cgacctcatc aagcagatcg ggaagaaggc catgaagaca agcgggggtg 1021 gggagacggg cccgggccca gggctggggc ctcaaggcgg tgggctccca gcactgcccc 1081 cttccctggt gcccctggta gtctactcca agaaccgggt gcccaccttg gaagaggtgt 1141 cacagacact gaggagcaaa ggccacgtat caaggtccCC AGATGAGGAG GCTGTGGCAC 1201 GGGCCCCTGA AGACAGCTCC CCTGCCCCCG AGGTGTTCAT GAACCAGCTG GACCGCATCA 1261 GCGAGGAATG CGAGCCATGG GACGCCCAGG ACTACCACCC ACTCATCTTC CAGGACGCCA 1321 GCATCACCTT CGTGAACACG GAGGCTGGCC TCTCAGACGA GGAGACCTCC AAGTCCTCGC 1381 TAGAGGACAA CTTGGCAGGG GAGGAGAGCC CCCAGCAGGG GGCTGAAGCC AAGGCGCCCC 1441 TGAACATGGG CGAGTTCCCC TCCTCCTCCG AGTCCACCTT CACCAGCACT GAGTCTGAGC 1501 TCTCTGTGCC TTACGAACAG CTGATGAATG AGTACAACAA GGCTAACAGC CCCAAGGGCA 1561 CATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1621 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1681 GGCTTTATAT ATCTTGTGGA AAGGACGACT CACCGCCTCG TGATCGCTTC TGACGCGTTA 1741 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt