Construct: ORF TRCN0000477846

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011427.1_s317c1
Derived from:
ccsbBroadEn_02298
DNA Barcode:
GTGGCCGACCACGGGTCATCTCTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
DNM1L (10059)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000477846
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10059 DNM1L dynamin 1 like NM_012063.3 100% 100%
2 human 10059 DNM1L dynamin 1 like NM_005690.4 98.4% 98.4% 1596_1597ins33
3 human 10059 DNM1L dynamin 1 like XM_011520543.3 98.2% 98.2% 250_288del
4 human 10059 DNM1L dynamin 1 like NM_001278463.1 97.2% 96.5% (many diffs)
5 human 10059 DNM1L dynamin 1 like NM_001330380.1 96.6% 96.6% 250_288del;1635_1636ins33
6 human 10059 DNM1L dynamin 1 like NM_012062.5 96.4% 96.4% 1593_1670del
7 human 10059 DNM1L dynamin 1 like NM_001278465.1 95.4% 94.8% (many diffs)
8 human 10059 DNM1L dynamin 1 like NM_001278464.1 94.7% 94.7% 250_288del;1632_1709del
9 human 10059 DNM1L dynamin 1 like XM_017018664.1 69.1% 69.1% 0_1ins657
10 human 10059 DNM1L dynamin 1 like XM_017018665.1 67.6% 67.6% 0_1ins657;939_940ins33
11 human 10059 DNM1L dynamin 1 like XM_017018663.2 67% 66.3% (many diffs)
12 human 10059 DNM1L dynamin 1 like XM_011520544.2 66.7% 66.7% 0_1ins657;936_1013del
13 human 10059 DNM1L dynamin 1 like NM_001278466.1 65.9% 61.4% (many diffs)
14 mouse 74006 Dnm1l dynamin 1-like XM_006522637.2 91.5% 98.7% (many diffs)
15 mouse 74006 Dnm1l dynamin 1-like NM_001276340.1 90.8% 97.9% (many diffs)
16 mouse 74006 Dnm1l dynamin 1-like NM_001025947.2 90% 97.3% (many diffs)
17 mouse 74006 Dnm1l dynamin 1-like XM_017317140.1 89.9% 96.9% (many diffs)
18 mouse 74006 Dnm1l dynamin 1-like XM_006522634.2 89.2% 96.1% (many diffs)
19 mouse 74006 Dnm1l dynamin 1-like XM_006522635.3 89% 95.4% (many diffs)
20 mouse 74006 Dnm1l dynamin 1-like NM_152816.3 88.4% 95.5% (many diffs)
21 mouse 74006 Dnm1l dynamin 1-like XM_006522633.2 88.3% 95.2% (many diffs)
22 mouse 74006 Dnm1l dynamin 1-like XM_006522636.2 87.7% 94.7% (many diffs)
23 mouse 74006 Dnm1l dynamin 1-like XM_006522631.3 87.6% 94.4% (many diffs)
24 mouse 74006 Dnm1l dynamin 1-like XM_006522632.3 87.4% 93.7% (many diffs)
25 mouse 74006 Dnm1l dynamin 1-like XM_006522629.3 86.8% 93.5% (many diffs)
26 mouse 74006 Dnm1l dynamin 1-like XM_006522630.3 86.7% 93% (many diffs)
27 mouse 74006 Dnm1l dynamin 1-like XM_006522628.3 86.1% 92.8% (many diffs)
28 mouse 74006 Dnm1l dynamin 1-like NM_001276341.1 79.1% 85% (many diffs)
29 mouse 74006 Dnm1l dynamin 1-like XM_006522638.3 70% 75.4% (many diffs)
30 mouse 74006 Dnm1l dynamin 1-like XM_017317141.1 52% 55.4% (many diffs)
31 mouse 74006 Dnm1l dynamin 1-like XM_006522639.1 51.8% 55.8% (many diffs)
32 mouse 74006 Dnm1l dynamin 1-like NR_075074.1 47.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2196
ORF length:
2130
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggcgctaatt cctgtcataa acaagctcca ggacgtcttc aacacggtgg
121gcgccgacat catccagctg cctcaaatcg tcgtagtggg aacgcagagc agcggaaaga
181gctcagtgct agaaagcctg gtggggaggg acctgcttcc cagaggtact ggaattgtca
241cccggagacc tctcattctg caactggtcc atgtttcaca agaagataaa cggaaaacaa
301caggagaaga aaatggggtg gaagcagaag aatggggtaa atttcttcac accaaaaata
361agctttacac ggattttgat gaaattcgac aagaaattga aaatgaaaca gaaagaattt
421caggaaataa taagggagta agccctgaac caattcatct taagattttt tcacccaacg
481ttgtcaattt gacacttgtg gatttgccag gaatgaccaa ggtgcctgta ggtgatcaac
541ctaaggatat tgagcttcaa atcagagagc tcattcttcg gttcatcagt aatcctaatt
601ccattatcct cgctgtcact gctgctaata cagatatggc aacatcagag gcacttaaaa
661tttcaagaga ggtagatcca gatggtcgca gaaccctagc tgtaatcact aaacttgatc
721tcatggatgc gggtactgat gccatggatg tattgatggg aagggttatt ccagtcaaac
781ttggaataat tggagtagtt aacaggagcc agctagatat taacaacaag aagagtgtaa
841ctgattcaat ccgtgatgag tatgcttttc ttcaaaagaa atatccatct ctggccaata
901gaaatggaac aaagtatctt gctaggactc taaacaggtt actgatgcat cacatcagag
961attgtttacc agagttgaaa acaagaataa atgttctagc tgctcagtat cagtctcttc
1021taaatagcta cggtgaaccc gtggatgata aaagtgctac tttactccaa cttattacca
1081aatttgccac agaatattgt aacactattg aaggaactgc aaaatatatt gaaacttcgg
1141agctatgcgg tggtgctaga atttgttata ttttccatga gacttttggg cgaaccttag
1201aatctgttga tccacttggt ggccttaaca ctattgacat tttgactgcc attagaaatg
1261ctactggtcc tcgtcctgct ttatttgtgc ctgaggtttc atttgagtta ctggtgaagc
1321ggcaaatcaa acgtctagaa gagcccagcc tccgctgtgt ggaactggtt catgaggaaa
1381tgcaaaggat cattcagcac tgtagcaatt acagtacaca ggaattgtta cgatttccta
1441aacttcatga tgccatagtt gaagtggtga cttgtcttct tcgtaaaagg ttgcctgtta
1501caaatgaaat ggtccataac ttagtggcaa ttgaactggc ttatatcaac acaaaacatc
1561cagactttgc tgatgcttgt gggctaatga acaataatat agaggaacaa aggagaaaca
1621ggctagccag agaattacct tcagctgtat cacgagacaa gttaattcag gacagcagaa
1681gagaaactaa aaatgttgca tctggaggtg gTGGGGTTGG AGATGGTGTT CAAGAACCAA
1741CCACAGGCAA CTGGAGAGGA ATGCTGAAAA CTTCAAAAGC TGAAGAGTTA TTAGCAGAAG
1801AAAAATCAAA ACCCATTCCA ATTATGCCAG CCAGTCCACA AAAAGGTCAT GCCGTGAACC
1861TGCTAGATGT GCCAGTTCCT GTTGCACGAA AACTATCTGC TCGGGAACAG CGAGATTGTG
1921AGGTTATTGA ACGACTCATT AAATCATATT TTCTCATTGT CAGAAAGAAT ATTCAAGACA
1981GTGTGCCAAA GGCAGTAATG CATTTTTTGG TTAATCATGT GAAAGACACT CTTCAGAGTG
2041AGCTAGTAGG CCAGCTGTAT AAATCATCCT TATTGGATGA TCTTCTGACA GAATCTGAGG
2101ACATGGCACA GCGCAGGAAA GAAGCAGCTG ATATGCTAAA GGCATTACAA GGAGCCAGTC
2161AAATTATTGC TGAAATCCGG GAGACTCATC TTTGGTGACC AACTTTCTTG TACAAAGTGG
2221TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2281CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2341AGTGGCCGAC CACGGGTCAT CTCTTACGCG TTAAGTCgac aatcaacctc tggattacaa
2401aatttgtgaa agatt

Download FASTA (ORF) (Full)