Transcript: Human XM_017018665.1

PREDICTED: Homo sapiens dynamin 1 like (DNM1L), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DNM1L (10059)
Length:
4118
CDS:
539..1981

Additional Resources:

NCBI RefSeq record:
XM_017018665.1
NBCI Gene record:
DNM1L (10059)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018665.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001097 GCTACTTTACTCCAACTTATT pLKO.1 872 CDS 100% 13.200 18.480 N DNM1L n/a
2 TRCN0000318424 GCTACTTTACTCCAACTTATT pLKO_005 872 CDS 100% 13.200 18.480 N DNM1L n/a
3 TRCN0000010594 CGGTGGTGCTAGAATTTGTTA pLKO.1 964 CDS 100% 5.625 7.875 N DNM1L n/a
4 TRCN0000318425 CGGTGGTGCTAGAATTTGTTA pLKO_005 964 CDS 100% 5.625 7.875 N DNM1L n/a
5 TRCN0000001099 CGAGATTGTGAGGTTATTGAA pLKO.1 1694 CDS 100% 5.625 4.500 N DNM1L n/a
6 TRCN0000318426 CGAGATTGTGAGGTTATTGAA pLKO_005 1694 CDS 100% 5.625 4.500 N DNM1L n/a
7 TRCN0000010593 GCACCAAGACAACATTTCATA pLKO.1 2363 3UTR 100% 5.625 3.938 N DNM1L n/a
8 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 3022 3UTR 100% 4.950 2.475 Y ERAP2 n/a
9 TRCN0000012607 CCTGCTTTATTTGTGCCTGAA pLKO.1 1091 CDS 100% 4.050 2.835 N Dnm1l n/a
10 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 3023 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018665.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02298 pDONR223 100% 67.6% 67.6% None 0_1ins657;939_940ins33 n/a
2 ccsbBroad304_02298 pLX_304 0% 67.6% 67.6% V5 0_1ins657;939_940ins33 n/a
3 TRCN0000477846 GTGGCCGACCACGGGTCATCTCTT pLX_317 23% 67.6% 67.6% V5 (not translated due to prior stop codon) 0_1ins657;939_940ins33 n/a
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