Construct: ORF TRCN0000477852
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010461.1_s317c1
- Derived from:
- ccsbBroadEn_14725
- DNA Barcode:
- GCTGCAAGAAGAAAACGAGCGTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- OSBP (5007)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477852
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5007 | OSBP | oxysterol binding protein | NM_002556.3 | 99.8% | 22.8% | (many diffs) |
| 2 | mouse | 76303 | Osbp | oxysterol binding protein | NM_001033174.1 | 90.2% | 21.1% | (many diffs) |
| 3 | mouse | 76303 | Osbp | oxysterol binding protein | XM_006527439.3 | 60.4% | 1.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgacggag ctgagaggag tggtggggcc aggcccggca gccattgcag 121 cacttggcgg cggcggcgcc ggtcccccag tggtgggagg aggcggcggc cgcggagatg 181 cggggccagg ctccggggcc gcgtcaggga cggtggtcgc ggcggcggcg ggaggcccgg 241 gcccgggggc cgggggagtg gcggcggctg gcccggcccc tgcgccgccg actgggggct 301 cgggcggctc gggcgctggg ggttcgggct cggctcgaga gggctggctc ttcaaatgga 361 ccaattatat caaaggctac cagcggcgat ggttcgtgct gagcaacggg ctcctgagct 421 actacagatc aaaggcagaa atgagacata cctgccgtgg taccatcaac ctcgccacag 481 ccaacatcac cgtggaggac tcctgcaact tcatcatttc caatgggggt gctcagacct 541 accatctgaa agctagttca gaagttgagc ggcagcgctg ggtgacggcc ctggaactgg 601 ccaaggccaa agctggtgaa gatgctggca gagtcagatg aatcaggaga tgaagagtct 661 gtctcacaaa ctgacaagac tgagctgcag aatacccttc ggaccctctc tagcaaagta 721 gaggacttga gcacgtgcaa tgacttgata gctaagcatg gcacagctct gcagcgttct 781 ctcagtgagc tggagtccct gaagttgcct gctgagagca atgaaaagat caaacaggtc 841 aacgaacgag ccacactctt taggataaca tccaatgcca tgatcaacgc ctgcagagat 901 ttcctcatgt tagcccagac ccatagtaaa aaatggcaaa agtcactaca gtatgaaaga 961 gaccagcgta tccgactgga agaaaccctc gagcagctgg cgaagcagca taatcacctg 1021 gagagggcct tccgaggagc cacggtgctg ccggcaaaca ctcctggcaa tgtgggttct 1081 ggtaaagatc agtgctgctc tggcaaaggg gacatgagcg atgaagatga tgagaatgaa 1141 ttttttgatg cacctgagat catcaccatg cctgaaaatt tgggccacaa acgtactggc 1201 agcaatatca gtggagccag cagtgacatc agccttgatg aacagtacaa gcatcagctg 1261 gaggagacca aaaaggaaaa gagaaccaga ataccataca agccaaacta tagcctcaat 1321 ttatggagca tcatgaagaa ctgcattgga aaagaactct ctaagatccc catgccggta 1381 aactttaatg agcccttgtc catgcttcag cgccttactg aagatctgga ataccatgag 1441 ctgttagacc gagctgcaaa atgtgagaat tctctagaac agctctgtta tgttgcagct 1501 ttcaccgtgt cctcctactc cactactgtc ttccgcacca gtaagccatt caacccactg 1561 cttggggaga cctttgagct ggaccgatta gaggagaatg ggtaccgatc cctctgtgaa 1621 caggtgagtc atcatccccc tgctgactgc gcaccatgct gagtccaaaa atggctggac 1681 attgcgtcag gaaatcaaaa tcaccagcaa gtttcgaggc aaatacctct ccattatgcc 1741 cctcggtacc attcattgta ttttccatgc aactgggcta ccactacact tggaagaaag 1801 ttaccacaac tgtacacaac attattgtgg gcaagttgtg gatagatcag tctggcgaaa 1861 ttgatattgt gaatcacaag acaggagaca agtgtaatct taaatttgtt ccttatagct 1921 acttctctcg ggatgtagca agaaaggtga cgggggaagt gacagatcca tcaggaaaag 1981 tccactttgc tcttctgggg acgtgggatg agaaaatgga atgtttcaaa gtacagccag 2041 tcattgggga aaatgggggt gatgctcgac agagaggcca tgaagcagag gaaagcaggg 2101 tcatgctgtg gaaaaggaat cctttaccga agaatgcaga aaacatgtac tacttctcag 2161 agcttgctct gactctcaat gcttgggaaa gtggcactgc ccccacagac agccggttac 2221 gacctgacca gagactgatg gaaaatggac gctgGGATGA AGCAAATGCG GAGAAGCAGC 2281 GCCTGGAGGA AAAACAAAGA CTTTCCAGAA AGAAGAGAGA AGCGGAAGCT ATGAAAGCCA 2341 CAGAGGATGG CACACCATAT GATCCCTATA AGGCACTGTG GTTTGAGCGG AAGAAGGACC 2401 CTGTTACCAA GGAGTTAACC CATATTTATA GGGGAGAATA CTGGGAGTGT AAAGAAAAAC 2461 AGGACTGGAG CTCATGCCCG GACATTTACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 2521 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2581 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCTGC 2641 AAGAAGAAAA CGAGCGTAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2701 tgaaagatt