Construct: ORF TRCN0000477859
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014136.1_s317c1
- Derived from:
- ccsbBroadEn_13703
- DNA Barcode:
- TCGCCTAGCCGTATGAGCCGAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPY19L2P4 (442523)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477859
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448957.1 | 32% | 25.1% | (many diffs) |
| 2 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448955.1 | 30.3% | 23.7% | (many diffs) |
| 3 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448954.1 | 29.4% | 23.1% | (many diffs) |
| 4 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448953.1 | 25.3% | 19.8% | (many diffs) |
| 5 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448952.1 | 24.4% | 19.1% | (many diffs) |
| 6 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448951.1 | 24.3% | 19.1% | (many diffs) |
| 7 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448950.1 | 23.6% | 18.5% | (many diffs) |
| 8 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448949.1 | 23.2% | 18.2% | (many diffs) |
| 9 | human | 442523 | DPY19L2P4 | DPY19L2 pseudogene 4 | NR_003551.1 | 22.8% | (many diffs) | |
| 10 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448948.1 | 22% | 17.2% | (many diffs) |
| 11 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_017019192.2 | 18.5% | 14.5% | (many diffs) |
| 12 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448945.1 | 18.3% | 14.4% | (many diffs) |
| 13 | human | 283417 | DPY19L2 | dpy-19 like 2 | XM_024448944.1 | 17.8% | 13.9% | (many diffs) |
| 14 | human | 283417 | DPY19L2 | dpy-19 like 2 | NM_173812.5 | 17.3% | 13.6% | (many diffs) |
| 15 | human | 554236 | DPY19L2P1 | DPY19L2 pseudogene 1 | NR_002833.2 | 13.8% | (many diffs) | |
| 16 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957316.1 | 10.6% | (many diffs) | |
| 17 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957315.1 | 10.2% | (many diffs) | |
| 18 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957314.1 | 9.6% | (many diffs) | |
| 19 | human | 349152 | DPY19L2P2 | DPY19L2 pseudogene 2 | NR_027768.1 | 9.1% | (many diffs) | |
| 20 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957318.1 | 3.7% | (many diffs) | |
| 21 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957319.1 | 3.5% | (many diffs) | |
| 22 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_001748666.2 | 3.5% | (many diffs) | |
| 23 | human | 283417 | DPY19L2 | dpy-19 like 2 | XR_002957317.1 | 3.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 534
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaaaaacaa ggagtaagcc cgaagccgct gcaatcttcc cgccccagcc 121 agtctaagcg gcggtgcggg cctcccccct tcccccccgc ctccgccccg gagccggagg 181 tagaggaggt ggaaaagtcg gCCCTAGGCG GCGGGAGGTC CTTCCGGCGG AGGATCCGAA 241 ATGTGGAAAA TCGAAAAGGC TTGGAGCTAA AGGTGGTGGC CAAGACCCTT CTTCTCGGCC 301 CCTTCCTGTT GGTCCGTAAT TCCCTGGCGC AGCTCCGGGA AGAGGTGCAC GAACTGCAGG 361 CGTGGTGGTT CCCCAGCAGA ACCACTTTGG ACTTCGCAGT CCTTGTGGCT TATCTACATT 421 GGTTACATTT AGTAAAACTT TGTGAAAATT ATCGTCATTT CTCTCACCTC TCATCTTTGG 481 AACGGGAGAT GACTTTTTGC ACCAAAATGG TTAGGTTTCT TCTTCTAACG AATTTGCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAT CGCCTAGCCG TATGAGCCGA AATACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att