Transcript: Human XM_024448953.1

PREDICTED: Homo sapiens dpy-19 like 2 (DPY19L2), transcript variant X21, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DPY19L2 (283417)
Length:
2120
CDS:
153..1712

Additional Resources:

NCBI RefSeq record:
XM_024448953.1
NBCI Gene record:
DPY19L2 (283417)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024448953.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000130196 CGCTGAGATACACAAAGACAT pLKO.1 1558 CDS 100% 4.950 2.970 N DPY19L2 n/a
2 TRCN0000128340 GCTGAGATACACAAAGACATT pLKO.1 1559 CDS 100% 4.950 2.970 N DPY19L2 n/a
3 TRCN0000129667 CCGAATTTGACTTCATGGAAA pLKO.1 1528 CDS 100% 4.950 2.475 Y DPY19L2 n/a
4 TRCN0000168247 CCCGAATTTGACTTCATGGAA pLKO.1 1527 CDS 100% 3.000 1.500 Y DPY19L2P2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024448953.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09943 pDONR223 100% 67.6% 66.4% None (many diffs) n/a
2 ccsbBroadEn_13716 pDONR223 100% 43.9% 37.3% None (many diffs) n/a
3 ccsbBroad304_13716 pLX_304 0% 43.9% 37.3% V5 (many diffs) n/a
4 TRCN0000470811 CCTGGTTGCCCCTCTAGGGCTTGA pLX_317 68.2% 43.9% 37.3% V5 (many diffs) n/a
5 ccsbBroadEn_13703 pDONR223 100% 25.3% 19.8% None (many diffs) n/a
6 ccsbBroad304_13703 pLX_304 0% 25.3% 19.8% V5 (many diffs) n/a
7 TRCN0000477859 TCGCCTAGCCGTATGAGCCGAAAT pLX_317 70.2% 25.3% 19.8% V5 (many diffs) n/a
8 ccsbBroadEn_10360 pDONR223 100% 21.6% 15.8% None (many diffs) n/a
9 ccsbBroad304_10360 pLX_304 0% 21.6% 15.8% V5 (many diffs) n/a
10 TRCN0000474402 ATCCGACGCAGTTTGAGACACATC pLX_317 100% 21.6% 15.8% V5 (many diffs) n/a
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