Transcript: Human XM_017019192.2

PREDICTED: Homo sapiens dpy-19 like 2 (DPY19L2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DPY19L2 (283417)
Length:
3758
CDS:
153..2279

Additional Resources:

NCBI RefSeq record:
XM_017019192.2
NBCI Gene record:
DPY19L2 (283417)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017019192.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000130196 CGCTGAGATACACAAAGACAT pLKO.1 1468 CDS 100% 4.950 2.970 N DPY19L2 n/a
2 TRCN0000128340 GCTGAGATACACAAAGACATT pLKO.1 1469 CDS 100% 4.950 2.970 N DPY19L2 n/a
3 TRCN0000434761 ATAGTGTGTACAGAGTATTAA pLKO_005 2248 CDS 100% 15.000 7.500 Y DPY19L2 n/a
4 TRCN0000128689 CCGTAATCAATGGAGCATAAT pLKO.1 1817 CDS 100% 13.200 6.600 Y DPY19L2 n/a
5 TRCN0000427668 CCTTACTTCACCACAGTATTT pLKO_005 2223 CDS 100% 13.200 6.600 Y DPY19L2 n/a
6 TRCN0000422868 GACGTGGGCAATAATTCTAAA pLKO_005 1214 CDS 100% 13.200 6.600 Y DPY19L2 n/a
7 TRCN0000128428 GCTCTGAAATGACATCCTTAA pLKO.1 2692 3UTR 100% 10.800 5.400 Y DPY19L2 n/a
8 TRCN0000167252 CCATTGTGAATCATCCACATT pLKO.1 1963 CDS 100% 4.950 2.475 Y DPY19L2P2 n/a
9 TRCN0000129667 CCGAATTTGACTTCATGGAAA pLKO.1 1438 CDS 100% 4.950 2.475 Y DPY19L2 n/a
10 TRCN0000167955 CATGTGTGTTATGGCTTCCTT pLKO.1 1691 CDS 100% 3.000 1.500 Y DPY19L2P2 n/a
11 TRCN0000168247 CCCGAATTTGACTTCATGGAA pLKO.1 1437 CDS 100% 3.000 1.500 Y DPY19L2P2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017019192.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09943 pDONR223 100% 93.1% 92.7% None (many diffs) n/a
2 ccsbBroadEn_13716 pDONR223 100% 32.2% 27.4% None (many diffs) n/a
3 ccsbBroad304_13716 pLX_304 0% 32.2% 27.4% V5 (many diffs) n/a
4 TRCN0000470811 CCTGGTTGCCCCTCTAGGGCTTGA pLX_317 68.2% 32.2% 27.4% V5 (many diffs) n/a
5 ccsbBroadEn_13703 pDONR223 100% 18.5% 14.5% None (many diffs) n/a
6 ccsbBroad304_13703 pLX_304 0% 18.5% 14.5% V5 (many diffs) n/a
7 TRCN0000477859 TCGCCTAGCCGTATGAGCCGAAAT pLX_317 70.2% 18.5% 14.5% V5 (many diffs) n/a
8 ccsbBroadEn_10360 pDONR223 100% 15.8% 11.6% None (many diffs) n/a
9 ccsbBroad304_10360 pLX_304 0% 15.8% 11.6% V5 (many diffs) n/a
10 TRCN0000474402 ATCCGACGCAGTTTGAGACACATC pLX_317 100% 15.8% 11.6% V5 (many diffs) n/a
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