Construct: ORF TRCN0000477880
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010300.1_s317c1
- Derived from:
- ccsbBroadEn_03184
- DNA Barcode:
- CGATATCTGTCACGTCTTCTCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST8SIA3 (51046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477880
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51046 | ST8SIA3 | ST8 alpha-N-acetyl-neuramin... | NM_015879.3 | 100% | 100% | |
| 2 | mouse | 20451 | St8sia3 | ST8 alpha-N-acetyl-neuramin... | NM_009182.3 | 92.2% | 97.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1209
- ORF length:
- 1140
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagaaactgc aaaatggccc gggtcgccag tgtgctgggg ctggtcatgc 121 tcagcgtcgc cctgctgatt ttatcgctca tcagctacgt gtccctgaaa aaggagaaca 181 tcttcaccac tcccaagtac gccagcccgg gggcgccccg aatgtacatg ttccacgcgg 241 gattccggtc acaatttgcg ctgaagtttc tagacccgtc attcgtgccc attacgaatt 301 ctctcaccca ggaactccaa gagaaacctt ctaagtggaa atttaatcgg acagcgtttt 361 tacatcaaag gcaagaaatt cttcagcatg tcgatgtaat aaaaaatttt tctttgacca 421 agaatagtgt tcggattgga caactgatgc actatgatta ttccagccat aaatatgttt 481 tctctattag caataacttc cggtcacttc ttccagatgt gtcacccatt atgaacaagc 541 attataatat ttgtgctgtg gttggaaata gtgggatcct gacagggagc cagtgtggac 601 aagaaataga taaatcagat tttgttttcc gttgcaattt cgcccctacg gaggctttcc 661 aaagagatgt tggaagaaaa accaatctta ccaccttcaa ccccagcatc ctggaaaaat 721 attacaacaa tctcttgact attcaggacc gtaacaactt tttcctcagt ttaaaaaagc 781 ttgacggggc cattctttgg atccctgcat ttttcttcca cacttcagca actgtgacca 841 ggacattagt tgactttTTT GTTGAACACA GAGGTCAGTT AAAAGTCCAA CTGGCTTGGC 901 CGGGAAATAT AATGCAACAT GTCAACAGGT ACTGGAAAAA CAAACATTTG TCACCTAAAC 961 GGCTGAGCAC AGGTATTCTT ATGTACACCC TTGCATCAGC AATATGTGAA GAGATCCACT 1021 TGTATGGATT TTGGCCGTTT GGATTTGACC CCAACACAAG GGAAGATCTT CCATACCATT 1081 ACTATGACAA AAAAGGAACC AAATTTACCA CCAAGTGGCA GGAGTCCCAC CAGCTGCCTG 1141 CTGAGTTTCA GCTGCTGTAC CGAATGCATG GGGAAGGGCT CACCAAGCTG ACTCTGTCAC 1201 ACTGTGCCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGATAT CTGTCACGTC TTCTCTTCAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt