Construct: ORF TRCN0000477885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010939.1_s317c1
- Derived from:
- ccsbBroadEn_09898
- DNA Barcode:
- TTTCCCCCCGACATGCCATGGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBC1D28 (254272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477885
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | NM_001039397.2 | 99.8% | 99.5% | 167A>C |
| 2 | human | 353149 | TBC1D26 | TBC1 domain family member 26 | NM_178571.4 | 79.2% | 73.6% | (many diffs) |
| 3 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_011523783.3 | 71.7% | 65.7% | (many diffs) |
| 4 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_011523781.2 | 63.8% | 60.3% | (many diffs) |
| 5 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_024450684.1 | 63.8% | 60.3% | (many diffs) |
| 6 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024422.1 | 58.8% | 51.8% | (many diffs) |
| 7 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024416.2 | 54% | 48% | (many diffs) |
| 8 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024417.2 | 54% | 48% | (many diffs) |
| 9 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024418.2 | 54% | 48% | (many diffs) |
| 10 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024419.2 | 54% | 48% | (many diffs) |
| 11 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024420.1 | 54% | 48% | (many diffs) |
| 12 | human | 254272 | TBC1D28 | TBC1 domain family member 28 | XM_017024421.2 | 54% | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 699
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagatggat gaggacccgg ataacctgcc tgcccagggg caaggcaata 121 tcatcattac taagtatgag cagggacacc gagctggggc agcagtggac ttggggcatg 181 agcaggttga tgtcagaaaa tacaccaata acctcgggat tgtgcatgag atggcgctgc 241 cccgcgtcag tgccctggag gtgaagcaaa gacgcaagga aagtaaacgt accaacaagt 301 ggcaaaagat gcttgcagac tggacaaaat ataggagcac caagaagctg tctcaaagag 361 tatgcaaagt cattcccctG GCGGTGCGGG GCCGGGCGTT GTCACTTTTG CTAGATATTG 421 ACAAAATCAA GTCCCAGAAC CCAGGCAAAT ATAAGGTCAT GAAGGAGAAG GGCAAGAGGT 481 CCTCCAGAAT CATCCACTGC ATCCAGCTAG ATGTCAGCCA CACCCTGCAG AAACACATGA 541 TGTTCATACA AAGATTCGGA GTCAAGCAGC AGGAATTATG TGACATCCTC GTGGCCTATT 601 CTGCATATAA CCCTGTGAGT ATTCCCGGGC AGCGATATTC CTGGTACCTG TGCCCATATT 661 CACAGGCATG GGTGTCTCTC GGGGGTGTTG CAACTTCTTT GCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGATTTCCC CCCGACATGC CATGGAATAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt