Construct: ORF TRCN0000477896
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008052.1_s317c1
- Derived from:
- ccsbBroadEn_09977
- DNA Barcode:
- TCCACCTTATGCACCGACGTTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF660 (285349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477896
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285349 | ZNF660 | zinc finger protein 660 | NM_173658.4 | 100% | 100% | |
| 2 | mouse | 382118 | Zkscan7 | zinc finger with KRAB and S... | XM_017313451.1 | 83.5% | 86% | (many diffs) |
| 3 | mouse | 382118 | Zkscan7 | zinc finger with KRAB and S... | XM_017313452.1 | 83.5% | 86% | (many diffs) |
| 4 | mouse | 382118 | Zkscan7 | zinc finger with KRAB and S... | XM_006512176.3 | 28% | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1062
- ORF length:
- 993
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggagaaag acaagaaatt tcaaacataa gacagttaaa gacaataaag 121 tactcacaga agggagtgac caagaatctg aaaaagacaa tagtcagtgc tgtgaccctg 181 caacaaatga gagagttcag gctgaaaaga gacagtatgt atgtactgag tgtgggaaag 241 cctttagtca gagtgcaaac ctcacagtac atgagcgaat ccacacggga gagaaaccct 301 ataagtgtaa ggagtgtgga aaagctttca gtcatagctc taaccttgtt gttcatcgga 361 gaatccacac tggactgaag ccctatacat gcagtgaatg tgggaaatct ttcagtggaa 421 agtcacatct tattcggcac cagggaatcc acagtgggga gaaaacttat gaatgtaaag 481 agtgtgggaa agcctttagt cggagttcgg gccttatatc acatcacaga gttcacaccg 541 gagagaagcc ctattcttgt attgagtgtg ggaaagcctt tagccgtagt tcaaacctta 601 ctcaacaTCA GCGAATGCAC AGAGGAAAAA AAGTTTACAA ATGTAAGGAG TGTGGGAAAA 661 CATGTGGTTC TAATACAAAG ATTATGGACC ATCAGAGAAT TCACACTGGA GAGAAGCCTT 721 ATGAATGTGA TGAGTGTGGA AAAACTTTCA TCTTAAGGAA AACTCTTAAT GAACACCAGA 781 GACTTCATCG TAGAGAGAAA CCTTACAAAT GTAATGAGTG TGGGAAGGCT TTTACTTCTA 841 ATCGAAACCT TGTTGATCAT CAGAGAGTTC ACACTGGAGA GAAACCCTAT AAATGTAATG 901 AATGTGGGAA AACCTTCAGG CAGACTTCTC AAGTTATTCT ACACTTGAGA ACCCACACTA 961 AGGAGAAACC CTATAAATGT AGTGAGTGTG GGAAAGCCTA TCGGTATAGT TCACAGCTTA 1021 TTCAACACCA GAGGAAACAT AATGAGGAGA AAGAAACCTC ATTGCCAACT TTCTTGTACA 1081 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1141 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1201 AGGACGATCC ACCTTATGCA CCGACGTTCC AACGCGTTAA GTCgacaatc aacctctgga 1261 ttacaaaatt tgtgaaagat t