Construct: ORF TRCN0000477923
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007987.1_s317c1
- Derived from:
- ccsbBroadEn_02609
- DNA Barcode:
- GAGATTATTCTGCCCGACCACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRNP35 (11066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477923
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11066 | SNRNP35 | small nuclear ribonucleopro... | NM_180699.3 | 100% | 100% | |
| 2 | human | 11066 | SNRNP35 | small nuclear ribonucleopro... | XM_017018724.2 | 100% | 100% | |
| 3 | human | 11066 | SNRNP35 | small nuclear ribonucleopro... | NM_022717.4 | 98% | 98% | 0_1insATGAAACCAGCTAAC |
| 4 | human | 11066 | SNRNP35 | small nuclear ribonucleopro... | XM_006719204.4 | 98% | 98% | 0_1insATGAAACCAGCTAAC |
| 5 | human | 11066 | SNRNP35 | small nuclear ribonucleopro... | XR_001748562.2 | 70.7% | (many diffs) | |
| 6 | human | 11066 | SNRNP35 | small nuclear ribonucleopro... | XR_001748561.2 | 68.8% | (many diffs) | |
| 7 | human | 11066 | SNRNP35 | small nuclear ribonucleopro... | XR_001748560.2 | 63.1% | (many diffs) | |
| 8 | mouse | 76167 | Snrnp35 | small nuclear ribonucleopro... | NM_029532.2 | 81.2% | 87.2% | (many diffs) |
| 9 | mouse | 76167 | Snrnp35 | small nuclear ribonucleopro... | XM_006530494.3 | 81.2% | 87.2% | (many diffs) |
| 10 | mouse | 76167 | Snrnp35 | small nuclear ribonucleopro... | XM_006530495.3 | 81.2% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 819
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa accagctaac atgaacgatt ggatgcccat cgccaaggag tatgatccac 121 tcaaagcggg cagcattgat ggcaccgatg aagacccaca cgaccgcgcg gtctggaggg 181 caatgctggc acgatatgtc cccaacaaag gtgtcatagg agatcccctc ctcaccctgt 241 ttgtggccag actaaacttg cagaccaagg aggacaaatt aaaggaagtc ttttcccgct 301 atggtgacat ccggcggctt cggctggtca gggacttggt cacaggtttt tcaaagggct 361 acgccTTCAT CGAATACAAG GAGGAGCGTG CCGTGATCAA AGCTTACCGA GATGCTGATG 421 GCCTGGTTAT TGACCAGCAT GAGATATTTG TGGACTACGA GCTGGAAAGG ACTCTCAAAG 481 GGTGGATCCC TCGGCGACTT GGAGGCGGTC TTGGGGGAAA AAAGGAGTCT GGGCAACTGA 541 GATTTGGGGG ACGGGACCGG CCTTTTCGAA AACCTATTAA CTTGCCAGTT GTTAAAAACG 601 ACCTCTATAG AGAGGGAAAA CGGGAAAGGC GGGAGCGATC TCGATCCCGA GAAAGACACT 661 GGGACTCGAG GACAAGGGAT CGAGACCATG ACAGGGGCCG GGAGAAGAGA TGGCAAGAAA 721 GAGAGCCGAC CAGGGTGTGG CCCGACAATG ACTGGGAGAG AGAGAGGGAC TTCAGAGATG 781 ACAGGATCAA GGGGAGGGAG AAGAAGGAAA GAGGCAAGTA CCCAACTTTC TTGTACAAAG 841 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 901 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 961 ACGAGAGATT ATTCTGCCCG ACCACTTTAC GCGTTAAGTC gacaatcaac ctctggatta 1021 caaaatttgt gaaagatt