Construct: ORF TRCN0000477942
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014708.1_s317c1
- Derived from:
- ccsbBroadEn_06729
- DNA Barcode:
- TGTGTCAAGTAATCTTTCTAGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIN4 (5303)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477942
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5303 | PIN4 | peptidylprolyl cis/trans is... | NM_006223.4 | 83.9% | 83.9% | 0_1ins75 |
| 2 | human | 5303 | PIN4 | peptidylprolyl cis/trans is... | NM_001170747.1 | 76.3% | 64.6% | (many diffs) |
| 3 | human | 5303 | PIN4 | peptidylprolyl cis/trans is... | NR_033187.2 | 26.9% | (many diffs) | |
| 4 | human | 728758 | PIN4P1 | peptidylprolyl cis/trans is... | NR_003571.1 | 18.5% | (many diffs) | |
| 5 | mouse | 69713 | Pin4 | protein (peptidyl-prolyl ci... | NM_027181.1 | 79.2% | 81.4% | (many diffs) |
| 6 | mouse | 105247253 | LOC105247253 | peptidyl-prolyl cis-trans i... | XM_011250928.1 | 79.2% | 81.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 537
- ORF length:
- 468
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcccatggcg gggcttctaa aggggcttgt acggcaactg gagcagttca 121 gagttcaaca acaagcttcc aagatgccgc ccaaaggaaa aagtggttct ggaaaagcgg 181 ggaaaggggg agcagcctct gggagtgaca gtgctgacaa gaaggctcaa ggtcccaaag 241 gtggtggcaa tgcagtaaag gtcagacaca ttcTATGTGA AAAACATGGC AAAATCATGG 301 AAGCCATGGA AAAGTTAAAG TCTGGGATGA GATTCAATGA AGTGGCCGCA CAGTATAGTG 361 AAGATAAAGC CAGGCAAGGG GGTGACTTGG GTTGGATGAC CAGAGGGTCC ATGGTGGGAC 421 CATTTCAAGA AGCAGCATTT GCCTTGCCTG TAAGTGGGAT GGATAAGCCT GTGTTTACAG 481 ACCCACCGGT TAAGACAAAA TTTGGATATC ATATTATTAT GGTCGAAGGA AGAAAATTGC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GATGTGTCAA GTAATCTTTC TAGAGGACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt