Construct: ORF TRCN0000477958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017321.1_s317c1
- Derived from:
- ccsbBroadEn_09941
- DNA Barcode:
- ACATATCTGCATCAGTTGGTCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASSF3 (283349)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477958
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283349 | RASSF3 | Ras association domain fami... | NM_178169.4 | 99.7% | 99.5% | 108A>G;698A>T |
| 2 | human | 283349 | RASSF3 | Ras association domain fami... | XM_006719345.3 | 90% | 81.1% | (many diffs) |
| 3 | human | 283349 | RASSF3 | Ras association domain fami... | XM_011538195.2 | 89.9% | 86.3% | (many diffs) |
| 4 | human | 283349 | RASSF3 | Ras association domain fami... | XM_017019182.1 | 86.4% | 84.4% | (many diffs) |
| 5 | human | 283349 | RASSF3 | Ras association domain fami... | NR_040718.2 | 13.5% | (many diffs) | |
| 6 | mouse | 192678 | Rassf3 | Ras association (RalGDS/AF-... | NM_138956.3 | 85.9% | 91.1% | (many diffs) |
| 7 | mouse | 192678 | Rassf3 | Ras association (RalGDS/AF-... | XM_006513379.1 | 59.6% | 64.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 783
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcagcggc tacagcagcc tggaggagga cgccgaggac ttcttcttca 121 ccgccaggac ctccttcttc aggagagcgc cccagggcaa gccccgctcc ggccagcaag 181 atgttgagaa agagaaggaa acccacagtt acctcagcaa agaggagatc aaagagaaag 241 ttcataaata caacttagca gtcacagaca agttgaagat gaccttgaat tcaaatggga 301 tTTACACTGG CTTCATTAAA GTACAGATGG AACTCTGCAA ACCTCCACAG ACTTCTCCAA 361 ATTCTGGAAA ACTCTCTCCC AGTAGCAATG GCTGTATGAA TACACTTCAT ATCAGCAGCA 421 CAAACACTGT CGGGGAAGTG ATCGAGGCCC TGCTCAAAAA GTTTCTCGTG ACTGAGAGCC 481 CTGCCAAGTT TGCACTTTAT AAGCGTTGTC ACAGGGAAGA CCAAGTCTAC GCCTGCAAGC 541 TCTCAGACCG GGAACATCCA CTCTACCTGC GTTTGGTAGC AGGGCCCAGA ACAGACACAC 601 TTAGTTTTGT TCTTCGTGAA CATGAAATTG GAGAGTGGGA AGCCTTCAGC CTTCCAGAAC 661 TACAGAATTT CTTGCGCATC TTGGACAAGG AAGAAGATGA ACAGCTGCAG AACCTGAAGA 721 GGCGCTACAC AGCCTACAGG CAGAAGCTGG AAGAAGCCCT CCGTGTGGTG TGGAAGCCTG 781 ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGAAC ATATCTGCAT CAGTTGGTCC TCACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt