Transcript: Human XM_011538195.2

PREDICTED: Homo sapiens Ras association domain family member 3 (RASSF3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RASSF3 (283349)
Length:
3351
CDS:
16..696

Additional Resources:

NCBI RefSeq record:
XM_011538195.2
NBCI Gene record:
RASSF3 (283349)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011538195.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077898 CGCCTTTCTATCTGTAGATTT pLKO.1 805 3UTR 100% 13.200 18.480 N RASSF3 n/a
2 TRCN0000423451 CATCCACTCTACCTGCGTTTG pLKO_005 466 CDS 100% 6.000 4.800 N RASSF3 n/a
3 TRCN0000436094 GTAGCAATGGCTGTATGAATA pLKO_005 293 CDS 100% 13.200 9.240 N RASSF3 n/a
4 TRCN0000433269 TACAGATGGAACTCTGCAAAC pLKO_005 233 CDS 100% 6.000 4.200 N RASSF3 n/a
5 TRCN0000428171 CACAGACTTCTCCAAATTCTG pLKO_005 257 CDS 100% 4.950 3.465 N RASSF3 n/a
6 TRCN0000077902 GAGATCAAAGAGAAAGTTCAT pLKO.1 136 CDS 100% 4.950 3.465 N RASSF3 n/a
7 TRCN0000431608 TAGCAGTCACAGACAAGTTGA pLKO_005 167 CDS 100% 4.950 3.465 N RASSF3 n/a
8 TRCN0000417538 AGAACCTGAAGAGGCGCTACA pLKO_005 620 CDS 100% 4.050 2.835 N RASSF3 n/a
9 TRCN0000419434 TTGCACTTTATAAGCGTTGTC pLKO_005 401 CDS 100% 4.050 2.835 N RASSF3 n/a
10 TRCN0000077901 CCTTGAATTCAAATGGGATTT pLKO.1 194 CDS 100% 0.000 0.000 N RASSF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011538195.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09941 pDONR223 100% 89.9% 86.3% None (many diffs) n/a
2 ccsbBroad304_09941 pLX_304 0% 89.9% 86.3% V5 (many diffs) n/a
3 TRCN0000477958 ACATATCTGCATCAGTTGGTCCTC pLX_317 66.7% 89.9% 86.3% V5 (many diffs) n/a
4 ccsbBroadEn_13498 pDONR223 100% 74.3% 74.3% None 1_174del n/a
5 ccsbBroad304_13498 pLX_304 0% 74.3% 74.3% V5 1_174del n/a
6 TRCN0000481294 GAGGTCCCAGATATCTCGGGACAC pLX_317 98.8% 74.3% 74.3% V5 1_174del n/a
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