Construct: ORF TRCN0000477989
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013469.1_s317c1
- Derived from:
- ccsbBroadEn_09239
- DNA Barcode:
- AACAGTACCCACGGACTGATAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM222A (84915)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477989
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84915 | FAM222A | family with sequence simila... | NM_032829.3 | 99.9% | 100% | 534C>A |
| 2 | human | 84915 | FAM222A | family with sequence simila... | XM_006719654.2 | 99.9% | 100% | 534C>A |
| 3 | human | 84915 | FAM222A | family with sequence simila... | XM_017020055.1 | 99.9% | 100% | 534C>A |
| 4 | human | 84915 | FAM222A | family with sequence simila... | XM_024449229.1 | 92.4% | 92.4% | 0_1ins102;432C>A |
| 5 | mouse | 433940 | Fam222a | family with sequence simila... | NM_001004180.1 | 85.1% | 90.9% | (many diffs) |
| 6 | mouse | 433940 | Fam222a | family with sequence simila... | XM_006530401.3 | 85.1% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1425
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggcctgt ctgcagagga cccagaacgc cccgggccaa cacctggcct 121 gcccgagcaa gagcctggag ctgcgcaagt gcgaggcggt ggccagcgcc atgcattcct 181 cccgctaccc gagcccagca gaactggacg cctatgccga gaaggtggcc aacagcccgc 241 tgtccatcaa gatcttcccc accaacatcc gtgtgcccca gcacaagcac ctcagccgca 301 cagtcaatgg ctatgacacc agtggccagc gctacagccc ctacccacag cacaccgctg 361 gctaccaggg ccttctggcc attgtcaagg ccgcggtttc ctcctccagc acggccgcac 421 cagctgggcc cgccaaaagt gtgctcaaga gcgccgaggg caagcggacc aagctgtcac 481 cggccgccgt gcaggtgggc attgcgccct acccagtgcc cagcactctg ggtcccttgg 541 cctaccccaa gccacctgag gcgcctgctc caccacccgg cctgcccgca gccgccactg 601 cagcctccgt catccccctg ccgggccggg gcctgcccct gccaccttcc aacctgccct 661 ccatccacag cctcctgtac cagctcaacc agcagtgcca ggccccgggc gccgcacccc 721 ctgcctgcca gggcatggct attccccatc ccagccctgc caagcacggc ccagtgccca 781 gcttccccag catggcctac tcggctgcag ccggtctgcc cgactgccgg aaaggcactg 841 agctgggcca gggagccacc caagccttga cgttggctgg ggccgccaag cctgcagggt 901 acgcagacag cggcctggat tacctgctgt ggccgcagaa accgccccca ccgccgcccc 961 agccactgcg tgcctacagt gggagcacgg tggccagcaa gtcccctgag gcttgcgggg 1021 gccgggcata cgagcgggcc agcgggtcac ccctcaacTG TGGCGTGGGG CTGCCCACCA 1081 GCTTCACCGT AGGCCAGTAC TTTGCGGCCC CGTGGAACAG TGTGCTGGTG ACACCCACCA 1141 GCGACTGCTA CAACCCAGCG GCGGCGGTGG TGGTCACGGA GCTGGGGCCG GGGGCAGCCC 1201 GGGAGCTGGC TGGGCCCCCT GCAGATGCCC TCTCGGGCCT GCCCAGCAAG AGTGTGTGCA 1261 ACACATCGGT GCTGAGCAGC AGCCTGCAGT CACTGGAGTA TCTCATCAAC GACATCCGGC 1321 CGCCCTGCAT CAAGGAGCAG ATGCTGGGCA AGGGCTATGA GACGGTGGCC GTGCCCCGGC 1381 TACTCGACCA CCAGCATGCC CACATCCGCC TACCCGTCTA CAGATTGCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1561 GAAAGGACGA AACAGTACCC ACGGACTGAT AAAAACGCGT TAAGTCgaca atcaacctct 1621 ggattacaaa atttgtgaaa gatt