Construct: ORF TRCN0000478001

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016377.1_s317c1
Derived from:
ccsbBroadEn_08794
DNA Barcode:
ACTTGACGTTACCGTTCGACTTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRMT11 (60487)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478001
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001031712.3 99.9% 99.7% 689T>A
2 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350580.1 97.3% 97.1% 71_72ins36;653T>A
3 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350581.1 86.3% 86.1% 0_1ins189;500T>A
4 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350582.1 86.3% 86.1% 0_1ins189;500T>A
5 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350583.1 86.3% 86.1% 0_1ins189;500T>A
6 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350584.1 86.3% 86.1% 0_1ins189;500T>A
7 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146798.1 67.9% (many diffs)
8 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146800.1 67.2% (many diffs)
9 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146796.1 67.1% (many diffs)
10 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146795.1 64.7% (many diffs)
11 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146801.1 64.4% (many diffs)
12 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146802.1 60.4% (many diffs)
13 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350585.1 57.1% 57% 0_1ins594;95T>A
14 human 60487 TRMT11 tRNA methyltransferase 11 h... XM_017011168.1 57.1% 57% 0_1ins594;95T>A
15 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350586.1 48.1% 44.9% (many diffs)
16 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350587.1 48.1% 44.9% (many diffs)
17 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350588.1 48.1% 44.9% (many diffs)
18 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350589.1 48.1% 44.9% (many diffs)
19 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350590.1 48.1% 44.9% (many diffs)
20 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350591.1 48.1% 44.9% (many diffs)
21 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350592.1 48.1% 44.9% (many diffs)
22 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350593.1 48.1% 44.9% (many diffs)
23 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350594.1 48.1% 44.9% (many diffs)
24 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350595.1 48.1% 44.9% (many diffs)
25 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350596.1 48.1% 44.9% (many diffs)
26 human 60487 TRMT11 tRNA methyltransferase 11 h... NM_001350597.1 48.1% 44.9% (many diffs)
27 human 60487 TRMT11 tRNA methyltransferase 11 h... XM_024446516.1 48.1% 44.9% (many diffs)
28 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146799.1 35.1% 1_121del;810T>A;1511_3953del
29 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146797.1 31.5% (many diffs)
30 human 60487 TRMT11 tRNA methyltransferase 11 h... XR_002956294.1 30.6% (many diffs)
31 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146804.2 30.1% (many diffs)
32 human 60487 TRMT11 tRNA methyltransferase 11 h... NR_146803.2 29.3% (many diffs)
33 mouse 73681 Trmt11 tRNA methyltransferase 11 NM_028604.2 89% 91.3% (many diffs)
34 mouse 73681 Trmt11 tRNA methyltransferase 11 XM_006512877.3 78.6% 80.9% (many diffs)
35 mouse 73681 Trmt11 tRNA methyltransferase 11 XM_011243211.2 77.6% 79.4% (many diffs)
36 mouse 73681 Trmt11 tRNA methyltransferase 11 XM_011243212.2 76.4% 77.7% (many diffs)
37 mouse 73681 Trmt11 tRNA methyltransferase 11 XM_006512880.3 51.4% 52.4% (many diffs)
38 mouse 73681 Trmt11 tRNA methyltransferase 11 XM_006512881.3 47.5% 46.1% (many diffs)
39 mouse 73681 Trmt11 tRNA methyltransferase 11 XM_006512882.3 47.3% 46.1% (many diffs)
40 mouse 73681 Trmt11 tRNA methyltransferase 11 XM_006512884.3 46% 47% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1458
ORF length:
1389
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcgctgtcg tgtaccctta acaggtatct gctcctcatg gcgcaggagc
121atctggagtt ccgcctgccg gaaataaagt ctttgctttt gctttttgga ggtcagtttg
181ccagcagtca agaaacttat ggaaagtcac cattttggat tcttagcatt ccctctgaag
241atattgcaag aaatttgatg aaacggacag tgtgtgccaa gtctatattt gaactatggg
301gtcatggaca atctcctgag gagctgtaca gttctcttaa aaactaccct gtggagaaga
361tggttccatt tctacattcg gactctacat ataaaataaa gattcacact tttaataaga
421cattgacaca agaagagaaa atcaagcgaa tagatgcact tgaatttctg ccatttgaag
481gaaaagtgaa tttaaagaaa ccgcaacatg tattttctgt tttggaggat tatggtttag
541acccaaactg catccctgag aatccacata atatttattt tggtagatgg attgcagatg
601gacagagaga gcttattgag tcatacagtg tcaaaaagag acactttatt ggaaatacaa
661gtatggatgc tggtttgtca ttcattatgg ctaaccatgg aaaagtgaaa gaaaatgata
721ttgtctttga tccatttgtt ggaacaggtg gcctgcagat agcatgtgct cattttggtg
781catatgtgta tgggacagac atagactaca acacagttca tggcttggga aaggctacta
841ggaaaaacca gaagtggaga ggaccagatg aaaacattag ggccaatctt cgtcaatatg
901gtttagagaa gtattacctt gatgtcctgg tttcagatgc atctaaacct tcctggagga
961agggcacata ttttgatgca atcattactg atcctccata tggtatcaga gaatctacaa
1021gaagaacagg ttcacagaag gagataccaa aggggataga aaaatgggaa aaatgtccag
1081aaagccatgt tcctgtttcc ttgagttatc atctgagtga tatgtttctt gacctgttaa
1141acttcgcagc tgagaccctc gttttaggtg gaagactagt cTATTGGTTA CCGGTGTATA
1201CGCCAGAATA CACTGAAGAG ATGGTGCCTT GGCACCCTTG CCTGGAACTC GTTAGCAACT
1261GCGAGCAGAA GCTTTCCAGT CACACATCAA GGCGCTTGAT CACAATGGAA AAGGTGAAGA
1321AATTTGAGAA TCGGGACCAG TATTCACATC TGCTAAGTGA TCATTTTCTG CCATACCAAG
1381GTCATAATTC CTTCCGTGAG AAATATTTTA GTGGGGTAAC AAAAAGAATT GCCAAGGAAG
1441AAAAATCCAC CCAGGAATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1501TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1561GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACTTGAC GTTACCGTTC
1621GACTTCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)