Construct: ORF TRCN0000478042

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003460.1_s317c1
Derived from:
ccsbBroadEn_00174
DNA Barcode:
TCTGAATTAGGGTTGATCCCCCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BRAF (673)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478042
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 673 BRAF B-Raf proto-oncogene, serin... NM_004333.6 100% 100%
2 human 673 BRAF B-Raf proto-oncogene, serin... NM_001354609.2 99.5% 99.3% (many diffs)
3 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374244.1 95% 95% 1175_1294del
4 human 673 BRAF B-Raf proto-oncogene, serin... NM_001374258.1 94.6% 94.4% (many diffs)
5 human 673 BRAF B-Raf proto-oncogene, serin... XM_017012559.1 89.3% 87.7% (many diffs)
6 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744857.1 48.1% (many diffs)
7 human 673 BRAF B-Raf proto-oncogene, serin... XR_001744858.1 22.1% (many diffs)
8 mouse 109880 Braf Braf transforming gene XM_006505359.3 82.4% 86.6% (many diffs)
9 mouse 109880 Braf Braf transforming gene XM_006505358.3 82.1% 86.2% (many diffs)
10 mouse 109880 Braf Braf transforming gene XM_011241139.2 81.8% 85.9% (many diffs)
11 mouse 109880 Braf Braf transforming gene NM_139294.5 80.9% 85% (many diffs)
12 mouse 109880 Braf Braf transforming gene XM_011241138.2 80.7% 84.7% (many diffs)
13 mouse 109880 Braf Braf transforming gene XM_011241136.2 80.7% 84.6% (many diffs)
14 mouse 109880 Braf Braf transforming gene XM_011241137.1 80.1% 84.1% (many diffs)
15 mouse 109880 Braf Braf transforming gene XM_006505355.3 78.5% 82.4% (many diffs)
16 mouse 109880 Braf Braf transforming gene XM_011241135.2 78.2% 82.1% (many diffs)
17 mouse 109880 Braf Braf transforming gene XM_006505353.3 77.5% 81.3% (many diffs)
18 mouse 109880 Braf Braf transforming gene XM_011241134.2 77.2% 81% (many diffs)
19 mouse 109880 Braf Braf transforming gene XM_011241140.2 56.8% 59.8% (many diffs)
20 mouse 109880 Braf Braf transforming gene XR_001785085.1 43.4% (many diffs)
21 mouse 109880 Braf Braf transforming gene XR_001785086.1 39.9% (many diffs)
22 mouse 109880 Braf Braf transforming gene XR_001785087.1 38.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2367
ORF length:
2298
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcggcgctg agcggtggcg gtggtggcgg cgcggagccg ggccaggctc
121tgttcaacgg ggacatggag cccgaggccg gcgccggcgc cggcgccgcg gcctcttcgg
181ctgcggaccc tgccattccg gaggaggtgt ggaatatcaa acaaatgatt aagttgacac
241aggaacatat agaggcccta ttggacaaat ttggtgggga gcataatcca ccatcaatat
301atctggaggc ctatgaagaa tacaccagca agctagatgc actccaacaa agagaacaac
361agttattgga atctctgggg aacggaactg atttttctgt ttctagctct gcatcaatgg
421ataccgttac atcttcttcc tcttctagcc tttcagtgct accttcatct ctttcagttt
481ttcaaaatcc cacagatgtg gcacggagca accccaagtc accacaaaaa cctatcgtta
541gagtcttcct gcccaacaaa cagaggacag tggtacctgc aaggtgtgga gttacagtcc
601gagacagtct aaagaaagca ctgatgatga gaggtctaat cccagagtgc tgtgctgttt
661acagaattca ggatggagag aagaaaccaa ttggttggga cactgatatt tcctggctta
721ctggagaaga attgcatgtg gaagtgttgg agaatgttcc acttacaaca cacaactttg
781tacgaaaaac gtttttcacc ttagcatttt gtgacttttg tcgaaagctg cttttccagg
841gtttccgctg tcaaacatgt ggttataaat ttcaccagcg ttgtagtaca gaagttccac
901tgatgtgtgt taattatgac caacttgatt tgctgtttgt ctccaagttc tttgaacacc
961acccaatacc acaggaagag gcgtccttag cagagactgc cctaacatct ggatcatccc
1021cttccgcacc cgcctcggac tctattgggc cccaaattct caccagtccg tctccttcaa
1081aatccattcc aattccacag cccttccgac cagcagatga agatcatcga aatcaatttg
1141ggcaacgaga ccgatcctca tcagctccca atgtgcatat aaacacaata gaacctgtca
1201atattgatga cttgattaga gaccaaggat ttcgtggtga tggaggatca accacaggtt
1261tgtctgctac cccccctgcc tcattacctg gctcactaac taacgtgaaa gccttacaga
1321aatctccagg acctcagcga gaaaggaagt catcttcatc ctcagaagac aggaatcgaa
1381tgaaaacact tggtagacgg gactcgagtg atgattggga gattcctgat gggcagatta
1441cagtgggaca aagaattgga tctggatcat ttggaacagt ctacaaggga aagtggcatg
1501gtgatgtggc agtgaaaatg ttgaatgtga cagcacctac acctcagcag ttacaagcct
1561tcaaaaatga agtaggagta ctcaggaaaa cacgacatgt gaatatccta ctcttcatgg
1621gctattccac aaagccacaa ctggctattg ttacccagtg gtgtgagggc tccagcttgt
1681atcaccatct ccatatcatt gagaccaaat ttgagatgat caaacttata gatattgcac
1741gacagactgc acagggcatg gattacttac acgccaagtc aatcatccac agagacctca
1801agagtaataa tatatttctt catgaagacc tcacagtaaa aataggtgat tttggtctag
1861ctacagtgaa atctcgatgg agtgggtccc atcagtttga acagttgtct ggatccattt
1921tgtggatggc accagaagtc atcagaatgc aagataaaaa TCCATACAGC TTTCAGTCAG
1981ATGTATATGC ATTTGGAATT GTTCTGTATG AATTGATGAC TGGACAGTTA CCTTATTCAA
2041ACATCAACAA CAGGGACCAG ATAATTTTTA TGGTGGGACG AGGATACCTG TCTCCAGATC
2101TCAGTAAGGT ACGGAGTAAC TGTCCAAAAG CCATGAAGAG ATTAATGGCA GAGTGCCTCA
2161AAAAGAAAAG AGATGAGAGA CCACTCTTTC CCCAAATTCT CGCCTCTATT GAGCTGCTGG
2221CCCGCTCATT GCCAAAAATT CACCGCAGTG CATCAGAACC CTCCTTGAAT CGGGCTGGTT
2281TCCAAACAGA GGATTTTAGT CTATATGCTT GTGCTTCTCC AAAAACACCC ATCCAGGCAG
2341GGGGATATGG TGCGTTTCCT GTCCACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
2401GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2461AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTGAATT
2521AGGGTTGATC CCCCGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2581aagatt

Download FASTA (ORF) (Full)