Construct: ORF TRCN0000478054
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009551.1_s317c1
- Derived from:
- ccsbBroadEn_14857
- DNA Barcode:
- CGCGCAATATACGCTCCCACCCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TCF3 (6929)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478054
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6929 | TCF3 | transcription factor 3 | NM_001136139.4 | 97.9% | 15% | (many diffs) |
| 2 | human | 6929 | TCF3 | transcription factor 3 | NM_001351779.2 | 97.9% | 15% | (many diffs) |
| 3 | human | 6929 | TCF3 | transcription factor 3 | XM_011528225.2 | 97.9% | 15% | (many diffs) |
| 4 | human | 6929 | TCF3 | transcription factor 3 | XM_011528226.2 | 97.8% | 15% | (many diffs) |
| 5 | human | 6929 | TCF3 | transcription factor 3 | NM_003200.5 | 96.8% | 14.9% | (many diffs) |
| 6 | human | 6929 | TCF3 | transcription factor 3 | XM_017027181.1 | 96.8% | 14.9% | (many diffs) |
| 7 | human | 6929 | TCF3 | transcription factor 3 | NM_001351778.2 | 96.6% | 14.9% | (many diffs) |
| 8 | human | 6929 | TCF3 | transcription factor 3 | XM_011528223.3 | 96.6% | 14.9% | (many diffs) |
| 9 | human | 6929 | TCF3 | transcription factor 3 | XM_006722855.4 | 94.3% | 15% | (many diffs) |
| 10 | human | 6929 | TCF3 | transcription factor 3 | XM_011528220.2 | 94.3% | 15% | (many diffs) |
| 11 | human | 6929 | TCF3 | transcription factor 3 | XM_017027178.1 | 94.3% | 15% | (many diffs) |
| 12 | human | 6929 | TCF3 | transcription factor 3 | XM_006722857.4 | 94.2% | 15% | (many diffs) |
| 13 | human | 6929 | TCF3 | transcription factor 3 | XM_006722858.3 | 94.2% | 15% | (many diffs) |
| 14 | human | 6929 | TCF3 | transcription factor 3 | XM_011528221.2 | 94.2% | 15% | (many diffs) |
| 15 | human | 6929 | TCF3 | transcription factor 3 | XM_017027179.2 | 94.1% | 15% | (many diffs) |
| 16 | human | 6929 | TCF3 | transcription factor 3 | XM_011528216.2 | 93.2% | 14.9% | (many diffs) |
| 17 | human | 6929 | TCF3 | transcription factor 3 | XM_017027177.2 | 93.2% | 14.9% | (many diffs) |
| 18 | human | 6929 | TCF3 | transcription factor 3 | XM_011528219.2 | 93% | 14.9% | (many diffs) |
| 19 | human | 6929 | TCF3 | transcription factor 3 | XM_024451679.1 | 92.9% | 14.3% | (many diffs) |
| 20 | human | 6929 | TCF3 | transcription factor 3 | XM_011528227.3 | 91.1% | 11.6% | (many diffs) |
| 21 | human | 6929 | TCF3 | transcription factor 3 | XM_024451675.1 | 90.7% | 14.4% | (many diffs) |
| 22 | human | 6929 | TCF3 | transcription factor 3 | XM_024451676.1 | 90.7% | 14.4% | (many diffs) |
| 23 | human | 6929 | TCF3 | transcription factor 3 | XM_024451677.1 | 90.6% | 14.4% | (many diffs) |
| 24 | human | 6929 | TCF3 | transcription factor 3 | XM_024451678.1 | 90.6% | 14.4% | (many diffs) |
| 25 | human | 6929 | TCF3 | transcription factor 3 | XM_024451669.1 | 89.6% | 14.3% | (many diffs) |
| 26 | human | 6929 | TCF3 | transcription factor 3 | XM_024451670.1 | 89.6% | 14.3% | (many diffs) |
| 27 | human | 6929 | TCF3 | transcription factor 3 | XM_024451671.1 | 89.6% | 14.3% | (many diffs) |
| 28 | human | 6929 | TCF3 | transcription factor 3 | XM_024451672.1 | 89.6% | 14.3% | (many diffs) |
| 29 | human | 6929 | TCF3 | transcription factor 3 | XM_024451673.1 | 89.6% | 14.3% | (many diffs) |
| 30 | human | 6929 | TCF3 | transcription factor 3 | XM_024451674.1 | 89.6% | 14.3% | (many diffs) |
| 31 | human | 6929 | TCF3 | transcription factor 3 | XM_017027180.2 | 83.5% | 10.6% | (many diffs) |
| 32 | human | 6929 | TCF3 | transcription factor 3 | XM_024451680.1 | 83.3% | 15.2% | (many diffs) |
| 33 | human | 6929 | TCF3 | transcription factor 3 | XR_002958351.1 | 43.4% | (many diffs) | |
| 34 | human | 6929 | TCF3 | transcription factor 3 | XR_001753747.1 | 42.9% | (many diffs) | |
| 35 | human | 6929 | TCF3 | transcription factor 3 | XR_001753743.2 | 42.6% | (many diffs) | |
| 36 | human | 6929 | TCF3 | transcription factor 3 | XR_002958347.1 | 41.8% | (many diffs) | |
| 37 | human | 6929 | TCF3 | transcription factor 3 | XR_002958348.1 | 41.8% | (many diffs) | |
| 38 | human | 6929 | TCF3 | transcription factor 3 | XR_002958350.1 | 40.3% | (many diffs) | |
| 39 | human | 6929 | TCF3 | transcription factor 3 | XR_001753745.1 | 39.6% | (many diffs) | |
| 40 | human | 6929 | TCF3 | transcription factor 3 | XR_002958349.1 | 39.3% | (many diffs) | |
| 41 | human | 6929 | TCF3 | transcription factor 3 | XR_002958346.1 | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 423
- ORF length:
- 354
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaccagccg cagaggatgg cgcctgtggg cacagacaag gagctcagtg 121 acctcctgga cttcagcatg atgttcccgc tgcctgtcac caacgggaag ggccggcccg 181 cctccctggc cggggcgcag ttcggaggtt caggtcttga ggaccggccc agctcaggct 241 cctggggcag cggcgaccag agcagctcct cctttgaccc cagccggacc ttcagcgagg 301 gcacccactt cactgagtcg cacagcagcc tctcttcatc cacattcatg tcggaggttc 361 aggcaagagc ggtgagcggg gcgcctatgc ctccttcggg agagacgcag gcgtgggcgg 421 cctgactcag gctggcttcc tgtcaggcga gctggccctc aacagccccg ggcccctgtc 481 cccttcgggc atgaagggga cctcccagta ctacccctcc tactccggca gctcccggcg 541 gagagcggca gacggcagcc tagacacgca gcccaagaag gtccggaagg tcccgccggg 601 tcttccatcc tcggtgtacc cacccagctc aggtgaggac tacggcaggg atgccaccgc 661 ctacccgtcc gccaagaccc ccagcagcac ctatcccgcc cccttctacg tggcagatgg 721 cagcctgcac ccctcagccg agctctggag tcccccgggc caggcgggct tcgggcccat 781 gctgggtggg ggctcatccc cgctgcccct cccgcccggt agcggcccgg tgggcagcag 841 tggaagcagc agcacgtttg gtggcctgca ccagcacgag cgtatgggct accagctgca 901 tggagcagag gtgaacggtg ggctcccatc tgcatcctcc ttctcctcag cccccggagc 961 cacgtacggc ggcgtctcca gccacacgcc gcctgtcagc ggggccgaca gcctcctggg 1021 ctcccgaggg accacagctg gcagctccgg ggatgccctc ggcaaagcac tggcctcgat 1081 ctactccccg gatcactcaa gcaataactt ctcgtccagc ccttctaccc ccgtgggctc 1141 cccccagggc ctggcaggaa cgtcacagtg gcctcgagca ggagcccccg gtgccttatc 1201 gcccagctac gacgggggtc tccacggcct gcagagtaag atagaagacc acctggacga 1261 ggccatccac gtgctccgca gccacgccgt gggcacagcc ggcgacatgc acacgctgct 1321 gcctggccac ggggcgctgg cctcaggttt caccggcccc atgtcactgg gcgggcggca 1381 cgcaggcctg gttggaggca gccaccccga ggacggcctc gcaggcagca ccagcctcat 1441 gcacaaccac gcggccctcc ccagccagcc aggcaccctc cctgacctgt ctcggcctcc 1501 cgactcctac agtgggctag ggcgagcagg tgccacggcg gccgccagcg agatcaagcg 1561 ggaggagaag gaggacgagg agaacacgtc agcggctgac cactcggagg aggagaagaa 1621 ggagctgaag gccccccggg cccggaccag gtgtacggac gaggtgctgt ccctggagga 1681 gaaagacctg agggaccggg agaggcgcat ggccaataac gcgcgggagc gggtgcgcgt 1741 gcgggatatt aacgaggcct tccgggagct ggggcgcatg tgccaatcgc acctcaacga 1801 gcagaagccc cagaccaaac tgctcatcct gcaccaggct gtcTCGGTCA TCCTTAACTT 1861 GGAGCAGCAA GTGCGAGAGC GGAACCTGAA TCCCAAAGCA GCCTGTTTGA AACGGCGAGA 1921 AGAGGAAAAG GTGTCAGGTG TGGTTGGAGA CCCCCAGATG GTGCTTTCAG CTCCCCACCC 1981 AGGCCTGAGC GAAGCCCACA ACCCCGCCGG GCACATGTTG CCAACTTTCT TGTACAAAGT 2041 GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG 2101 AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 2161 CGACGCGCAA TATACGCTCC CACCCCCACG CGTTAAGTCg acaatcaacc tctggattac 2221 aaaatttgtg aaagatt