Transcript: Human NM_003200.5

Homo sapiens transcription factor 3 (TCF3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
TCF3 (6929)
Length:
4735
CDS:
356..2320

Additional Resources:

NCBI RefSeq record:
NM_003200.5
NBCI Gene record:
TCF3 (6929)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003200.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000017534 CCCGGATCACTCAAGCAATAA pLKO.1 1378 CDS 100% 13.200 18.480 N TCF3 n/a
2 TRCN0000274218 CCCGGATCACTCAAGCAATAA pLKO_005 1378 CDS 100% 13.200 18.480 N TCF3 n/a
3 TRCN0000017536 GCCTGTTTGAAACGGCGAGAA pLKO.1 2201 CDS 100% 4.050 5.670 N TCF3 n/a
4 TRCN0000274219 GCCTGTTTGAAACGGCGAGAA pLKO_005 2201 CDS 100% 4.050 5.670 N TCF3 n/a
5 TRCN0000274217 ATTGTGCCTAAGCGAAATATT pLKO_005 2805 3UTR 100% 15.000 10.500 N TCF3 n/a
6 TRCN0000017533 CCTGGGAGTTTGATCTCTTAA pLKO.1 4361 3UTR 100% 13.200 9.240 N TCF3 n/a
7 TRCN0000274216 ACGGCCTGCAGAGTAAGATAG pLKO_005 1515 CDS 100% 10.800 7.560 N TCF3 n/a
8 TRCN0000086616 CCTGGACTTCAGCATGATGTT pLKO.1 412 CDS 100% 4.950 3.465 N Tcf3 n/a
9 TRCN0000017537 CCCGACTCCTACAGTGGGCTA pLKO.1 1790 CDS 100% 0.000 0.000 N TCF3 n/a
10 TRCN0000017535 CAGCCTCTCTTCATCCACATT pLKO.1 613 CDS 100% 4.950 2.970 N TCF3 n/a
11 TRCN0000274215 CAGCCTCTCTTCATCCACATT pLKO_005 613 CDS 100% 4.950 2.970 N TCF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003200.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000478054 CGCGCAATATACGCTCCCACCCCC pLX_317 9.3% 96.8% 14.9% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14857 pDONR223 87.5% 96.6% 14.9% None (many diffs) n/a
3 ccsbBroad304_14857 pLX_304 0% 96.6% 14.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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