Construct: ORF TRCN0000478063
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016724.1_s317c1
- Derived from:
- ccsbBroadEn_01585
- DNA Barcode:
- CTGGGAGATCTCTGGGCAATCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPAST (6683)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478063
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6683 | SPAST | spastin | NM_199436.1 | 100% | 100% | |
| 2 | human | 6683 | SPAST | spastin | NM_001363875.1 | 99.8% | 99.8% | 413_414insAGC |
| 3 | human | 6683 | SPAST | spastin | NM_014946.3 | 94.8% | 94.8% | 585_680del |
| 4 | human | 6683 | SPAST | spastin | NM_001363823.1 | 94.6% | 94.6% | 413_414insAGC;582_677del |
| 5 | human | 6683 | SPAST | spastin | XM_017004778.2 | 88.8% | 87.1% | 1518_1519ins71;1557_1558ins124 |
| 6 | human | 6683 | SPAST | spastin | XM_011533067.2 | 84.2% | 82.6% | 585_680del;1614_1615ins71;1653_1654ins124 |
| 7 | mouse | 50850 | Spast | spastin | XM_006524610.3 | 88.3% | 92.8% | (many diffs) |
| 8 | mouse | 50850 | Spast | spastin | XM_006524611.3 | 88.1% | 92.6% | (many diffs) |
| 9 | mouse | 50850 | Spast | spastin | NM_001162870.1 | 83.7% | 87.9% | (many diffs) |
| 10 | mouse | 50850 | Spast | spastin | NM_016962.2 | 83.5% | 87.8% | (many diffs) |
| 11 | mouse | 50850 | Spast | spastin | XM_011246530.2 | 52.3% | 57.3% | (many diffs) |
| 12 | mouse | 50850 | Spast | spastin | XR_001782088.1 | 24.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1821
- ORF length:
- 1752
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaattctccg ggtggacgag ggaagaagaa aggctccggc ggcgccagca 121 acccggtgcc tcccaggcct ccgccccctt gcctggcccc cgcccctccc gccgccgggc 181 cggcccctcc gcccgagtcg ccgcataagc ggaacctgta ctatttctcc tacccgctgt 241 ttgtaggctt cgcgctgctg cgtttggtcg ccttccacct ggggctcctc ttcgtgtggc 301 tctgccagcg cttctcccgc gccctcatgg cagccaagag gagctccggg gccgcgccag 361 cacctgcctc ggcctcggcc ccggcgccgg tgccgggcgg cgaggccgag cgcgtccgag 421 tcttccacaa acaggccttc gagtacatct ccattgccct gcgcatcgat gaggatgaga 481 aagcaggaca gaaggagcaa gctgtggaat ggtataagaa aggtattgaa gaactggaaa 541 aaggaatagc tgttatagtt acaggacaag gtgaacagtg tgaaagagct agacgccttc 601 aagctaaaat gatgactaat ttggttatgg ccaaggaccg cttacaactt ctagaaagtg 661 gagctgttcc aaaaagaaaa gaccccttaa cacacactag taattcactg cctcgttcaa 721 aaacagttat gaaaactgga tctgcaggcc tttcaggcca ccatagagca cctagttaca 781 gtggtttatc catggtttct ggagtgaaac agggatctgg tcctgctcct accactcata 841 agggtactcc gaaaacaaat aggacaaata aaccttctac ccctacaact gctactcgta 901 agaaaaaaga cttgaagaat tttaggaatg tggacagcaa ccttgctaac cttataatga 961 atgaaattgt ggacaatgga acagctgtta aatttgatga tatagctggt caagacttgg 1021 caaaacaagc attgcaagaa attgttattc ttccttctct gaggcctgag ttgttcacag 1081 ggcttagagc tcctgccaga gggctgttac tctttggtcc acctgggaat gggaagacaa 1141 tgctggctaa agcagtagct gcagaatcga atgcaacctt ctttaatata agtgctgcaa 1201 gtttaacttc aaaatacgtg ggagaaggag agaaattggt gagggctctt tttgctgtgg 1261 ctcgagaact tcaaccttct ataattttta tagatgaagt tgatagcctt ttgtgtgaaa 1321 gaagagaagg ggagcacgat gctagtagac gcctaaaaac tgaatttcta atagaatttG 1381 ATGGTGTACA GTCTGCTGGA GATGACAGAG TACTTGTAAT GGGTGCAACT AATAGGCCAC 1441 AAGAGCTTGA TGAGGCTGTT CTCAGGCGTT TCATCAAACG GGTATATGTG TCTTTACCAA 1501 ATGAGGAGAC AAGACTACTT TTGCTTAAAA ATCTGTTATG TAAACAAGGA AGTCCATTGA 1561 CCCAAAAAGA ACTAGCACAA CTTGCTAGAA TGACTGATGG ATACTCAGGA AGTGACCTAA 1621 CAGCTTTGGC AAAAGATGCA GCACTGGGTC CTATCCGAGA ACTAAAACCA GAACAGGTGA 1681 AGAATATGTC TGCCAGTGAG ATGAGAAATA TTCGATTATC TGACTTCACT GAATCCTTGA 1741 AAAAAATAAA ACGCAGCGTC AGCCCTCAAA CTTTAGAAGC GTACATACGT TGGAACAAGG 1801 ACTTTGGAGA TACCACTGTT TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTGG GAGATCTCTG 1981 GGCAATCGTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt