Construct: ORF TRCN0000478068
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007131.1_s317c1
- Derived from:
- ccsbBroadEn_12710
- DNA Barcode:
- GACAGACTTCTTAGGATCCTTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LNPK (80856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478068
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80856 | LNPK | lunapark, ER junction forma... | NM_001305011.2 | 100% | 100% | |
| 2 | human | 80856 | LNPK | lunapark, ER junction forma... | XM_017005055.1 | 100% | 100% | |
| 3 | human | 80856 | LNPK | lunapark, ER junction forma... | XM_005246880.3 | 98.3% | 98.3% | 1_15del |
| 4 | human | 80856 | LNPK | lunapark, ER junction forma... | XM_005246879.4 | 86.4% | 86.4% | 1_144del |
| 5 | human | 80856 | LNPK | lunapark, ER junction forma... | XM_011511945.2 | 86.4% | 86.4% | 1_144del |
| 6 | human | 80856 | LNPK | lunapark, ER junction forma... | NM_030650.3 | 71.2% | 71.2% | 1_369del |
| 7 | human | 80856 | LNPK | lunapark, ER junction forma... | XM_006712783.2 | 71.2% | 71.2% | 1_369del |
| 8 | human | 80856 | LNPK | lunapark, ER junction forma... | NM_001305010.1 | 70.9% | 70.9% | 1_375del |
| 9 | human | 80856 | LNPK | lunapark, ER junction forma... | NM_001305009.1 | 66.4% | 66.4% | 1_369del;706_798del |
| 10 | human | 80856 | LNPK | lunapark, ER junction forma... | NM_001305008.1 | 61.7% | 61.7% | 1_567del |
| 11 | human | 80856 | LNPK | lunapark, ER junction forma... | XM_005246878.4 | 58.3% | 54.6% | (many diffs) |
| 12 | human | 80856 | LNPK | lunapark, ER junction forma... | NR_130941.2 | 11.4% | 1_887del;1572_1646del;1878_7965del | |
| 13 | mouse | 69605 | Lnpk | lunapark, ER junction forma... | XM_006500147.3 | 85.5% | 81.4% | (many diffs) |
| 14 | mouse | 69605 | Lnpk | lunapark, ER junction forma... | XM_011239769.2 | 85.5% | 81.4% | (many diffs) |
| 15 | mouse | 69605 | Lnpk | lunapark, ER junction forma... | NM_027133.3 | 61% | 58.1% | (many diffs) |
| 16 | mouse | 69605 | Lnpk | lunapark, ER junction forma... | XM_006500145.3 | 61% | 58.1% | (many diffs) |
| 17 | mouse | 69605 | Lnpk | lunapark, ER junction forma... | XM_006500146.3 | 61% | 58.1% | (many diffs) |
| 18 | mouse | 69605 | Lnpk | lunapark, ER junction forma... | NM_001110209.1 | 50.4% | 46.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 984
- ORF length:
- 915
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaaaagaa acttacaaga cggctaaatt aattcttgaa aggtttgatc 121 cggactcaaa gaaagcaaag gagtgtgagc cgccatctgc tggagcagct gtaactgcaa 181 gacctggaca agagattcgt cagcgaactg cagctcaaag aaacctttct ccaacaccag 241 caagccctaa ccagggccct cctccacaag ttccagtatc tcctggacca ccaaaggaca 301 gttctgcccc tggtggaccc ccagaaagga ctgttactcc agccctatca tcaaatgtgt 361 taccaagaca tcttggatcc cctgctactt cagtgcctgg aatgggtctt catcctccag 421 gtccaccttt agcaagacct attctccccc gagaacgagg tgctttggat agaattgttg 481 aatatttggt tggtgatggt ccacaaaaca ggtatgcact tatatgtcag cagtgttttt 541 ctcataatgg catggctttg aaggaagaat ttgaatacat tgcttttcga tgtgcctact 601 gttttttctt gaaccctgca agaaaaacca gacctcaggc tccaagactt cctgagttta 661 gttttgagaa gaggcaggtg gtggaaggtt caagttcagt tggtcccttg ccatcaggaa 721 gtgtgctttc atcagacaac cagtttaatg aagaatcttt agaacacgat gttcttGATG 781 ATAATACAGA GCAGACAGAT GACAAAATAC CAGCTACAGA ACAGACAAAC CAAGTGATTG 841 AAAAAGCATC TGACTCAGAG GAACCAGAGG AGAAACAAGA GACTGAGAAT GAGGAAGCCT 901 CAGTGATTGA AACCAACTCC ACAGTTCCTG GAGCTGATTC TATTCCTGAT CCTGAACTAA 961 GTGGAGAATC TTTGACGGCA GAGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1021 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1081 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG ACAGACTTCT 1141 TAGGATCCTT CGAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1201 att