Transcript: Human NM_030650.3

Homo sapiens lunapark, ER junction formation factor (LNPK), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
LNPK (80856)
Length:
7542
CDS:
171..1457

Additional Resources:

NCBI RefSeq record:
NM_030650.3
NBCI Gene record:
LNPK (80856)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_030650.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000279675 ACGATGTTCTTGATGATAATA pLKO_005 1237 CDS 100% 15.000 21.000 N LNPK n/a
2 TRCN0000248060 ATTATGGGTTGGAAGATTAAT pLKO_005 290 CDS 100% 15.000 21.000 N Lnpk n/a
3 TRCN0000128745 CGTGCCTTCAACTGGATATTT pLKO.1 1470 3UTR 100% 15.000 21.000 N LNPK n/a
4 TRCN0000279674 TTTGACGGCAGAGTAGTAAAT pLKO_005 1442 CDS 100% 13.200 18.480 N LNPK n/a
5 TRCN0000248061 ATGAAGCATTGGATGATTTAA pLKO_005 487 CDS 100% 15.000 10.500 N Lnpk n/a
6 TRCN0000279604 GTATGTATAACCCGTAAATAT pLKO_005 1760 3UTR 100% 15.000 10.500 N LNPK n/a
7 TRCN0000129814 CTGGAGCATAAGAACAGTAAT pLKO.1 431 CDS 100% 13.200 9.240 N LNPK n/a
8 TRCN0000130072 GAAACTTACAAGACGGCTAAA pLKO.1 549 CDS 100% 10.800 7.560 N LNPK n/a
9 TRCN0000279672 GAAACTTACAAGACGGCTAAA pLKO_005 549 CDS 100% 10.800 7.560 N LNPK n/a
10 TRCN0000128139 CAGACAAACCAAGTGATTGAA pLKO.1 1293 CDS 100% 5.625 3.938 N LNPK n/a
11 TRCN0000279603 CAGACAAACCAAGTGATTGAA pLKO_005 1293 CDS 100% 5.625 3.938 N LNPK n/a
12 TRCN0000128787 CCTGAACTAAGTGGAGAATCT pLKO.1 1422 CDS 100% 4.950 3.465 N LNPK n/a
13 TRCN0000130543 GCTTTCATCAGACAACCAGTT pLKO.1 1196 CDS 100% 4.050 2.835 N LNPK n/a
14 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 5645 3UTR 100% 10.800 5.400 Y SMIM11A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030650.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12710 pDONR223 100% 71.2% 71.2% None 1_369del n/a
2 ccsbBroad304_12710 pLX_304 0% 71.2% 71.2% V5 1_369del n/a
3 TRCN0000478068 GACAGACTTCTTAGGATCCTTCGA pLX_317 23.2% 71.2% 71.2% V5 1_369del n/a
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