Construct: ORF TRCN0000478072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002354.1_s317c1
- Derived from:
- ccsbBroadEn_03466
- DNA Barcode:
- GAGTGCCCCGGCTGTTTGGCGAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BIVM (54841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478072
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54841 | BIVM | basic, immunoglobulin-like ... | NM_017693.4 | 100% | 100% | |
| 2 | human | 54841 | BIVM | basic, immunoglobulin-like ... | NM_001159596.2 | 53.7% | 53.7% | 0_1ins687;530_550del |
| 3 | human | 100533467 | BIVM-ERCC5 | BIVM-ERCC5 readthrough | NM_001204425.1 | 30.1% | 29.8% | (many diffs) |
| 4 | mouse | 246229 | Bivm | basic, immunoglobulin-like ... | NM_144558.4 | 87% | 87.4% | (many diffs) |
| 5 | mouse | 246229 | Bivm | basic, immunoglobulin-like ... | XM_006495998.3 | 87% | 87.4% | (many diffs) |
| 6 | mouse | 246229 | Bivm | basic, immunoglobulin-like ... | XM_006496000.2 | 87% | 87.4% | (many diffs) |
| 7 | mouse | 246229 | Bivm | basic, immunoglobulin-like ... | XM_006496002.2 | 87% | 87.4% | (many diffs) |
| 8 | mouse | 246229 | Bivm | basic, immunoglobulin-like ... | XM_006496004.3 | 87% | 87.4% | (many diffs) |
| 9 | mouse | 246229 | Bivm | basic, immunoglobulin-like ... | XM_017320721.1 | 87% | 87.4% | (many diffs) |
| 10 | mouse | 246229 | Bivm | basic, immunoglobulin-like ... | XM_017320726.1 | 86.8% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1578
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctaacgtt gcagaaacag aaaggtcaaa tgattctgga aatggtgagc 121 acaaatctga gagaaagtca cctgaagaga atctacaagg tgctgtaaaa tctttctgca 181 caagtgcctc aggagcaccc ttgggtccca aaggagatgg tcattatcca tggagttgtc 241 cagtgactca tacacgggaa aaaatttatg ccatctgttc ggactatgcc tttctcaacc 301 aggcgacctc aatctataaa actccaaatc catcccgctc tccttgcctc cctgatagta 361 cctctttatc tgctggaaat aattcatcaa gatacattgg tatcccgact agtacatcgg 421 aaattatcta caatgaagaa aatagcttgg aaaacttatc caacagcctg ggcaagctac 481 ctctcgcatg ggaaattgat aaatctgaat ttgatggggt gaccacaaat tcgaaacaca 541 aatcaggcaa tgcaaagaaa caagtttcca agagaaaaac ttcagataaa aagggaagat 601 atcagaagga atgtcctcag cattctcctc ttgaagatat taaacagcgg aaagtattag 661 acctcagacg atggtactgc ataagccgac cacagtataa gacttcttgt ggcatctctt 721 cattaatttc ttgttggaat ttcttataca gcacaatggg agctggaaac cttccaccta 781 ttacccaaga agaagcttta catattctgg gctttcaacc tccatttgaa gatattaggt 841 ttggtccttt cacggggaat acaacactta tgaggtggtt tagacaaatt aatgaccact 901 tccatgtaaa aggatgctct tatgttctat ataagcctca tgggaagaat aaaacagcag 961 gagaaactgc ttcaggggcc ctgtcaaagt taacccgtgg attgaaagat gaatcgctgg 1021 cttatatcta tcattgccaa aatcattatt tttgtccaat tggcttcgaa gcaacccctg 1081 ttaaagctaa taaagcattc agcaggggac ctctctcacc acaggaagtt gaatattgga 1141 tcttaattgg agaatcaagt agaaaacatc ctgccattca ctgtaaaaaa tgggcagata 1201 ttgttactga tctaaacact caaaaTCCAG AATACCTGGA TATCCGGCAC TTAGAGAGGG 1261 GACTGCAGTA TAGAAAAACA AAGAAGGTTG GGGGAAATTT GCATTGCATC ATAGCATTCC 1321 AGAGACTTAA CTGGCAAAGA TTTGGCCTTT GGAACTTTCC ATTTGGAACC ATTAGACAAG 1381 AATCACAACC TCCAACACAT GCCCAGGGAA TTGCCAAATC TGAGAGTGAA GACAATATTT 1441 CCAAGAAGCA GCATGGGCGT CTGGGCCGGT CTTTCAGTGC TAGTTTCCAT CAGGACTCGG 1501 CATGGAAAAA GATGTCTAGT ATCCATGAGA GAAGGAACAG TGGTTACCAG GGTTACAGTG 1561 ATTACGATGG GAATGATTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAGTGCC CCGGCTGTTT 1741 GGCGAGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt