Construct: ORF TRCN0000478100
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004715.1_s317c1
- Derived from:
- ccsbBroadEn_13032
- DNA Barcode:
- ATGCTTCTTACTTAGTGCGTGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CGB2 (114336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478100
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3972 | LHB | luteinizing hormone subunit... | XM_011526975.1 | 70% | 16.9% | (many diffs) |
| 2 | human | 3972 | LHB | luteinizing hormone subunit... | NM_000894.2 | 69.3% | 18.4% | (many diffs) |
| 3 | human | 114335 | CGB1 | chorionic gonadotropin subu... | NM_033377.1 | 67.3% | 18.6% | 0_1ins49;10A>G;348_465del |
| 4 | human | 114336 | CGB2 | chorionic gonadotropin subu... | XM_005258479.3 | 65.8% | 17.6% | (many diffs) |
| 5 | human | 114336 | CGB2 | chorionic gonadotropin subu... | NM_033378.1 | 64.4% | 19.3% | 0_1ins49;348_489del |
| 6 | human | 1082 | CGB3 | chorionic gonadotropin subu... | NM_000737.3 | 64.4% | 19% | (many diffs) |
| 7 | human | 93659 | CGB5 | chorionic gonadotropin subu... | NM_033043.2 | 64.4% | 19% | (many diffs) |
| 8 | human | 94115 | CGB8 | chorionic gonadotropin subu... | NM_033183.2 | 64.4% | 19% | (many diffs) |
| 9 | human | 94027 | CGB7 | chorionic gonadotropin subu... | NM_033142.1 | 63.9% | 18.6% | (many diffs) |
| 10 | human | 94027 | CGB7 | chorionic gonadotropin subu... | XM_024451784.1 | 63.9% | 18.6% | (many diffs) |
| 11 | human | 114336 | CGB2 | chorionic gonadotropin subu... | NM_001319065.1 | 57.8% | 19.8% | 0_1ins85;312_453del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 465
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccacatcc ccagtgcttg cggaagatat cccgctaaga gagagacatg 121 tcaaaggggc tgcTGCTGTT GCTGCTGCTG AGCATGGGCG GGACATGGGC ATCCAAGGAG 181 CCGCTTCGGC CACGGTGCCG CCCCATCAAT GCCACCCTGG CTGTGGAGAA GGAGGGCTGC 241 CCCGTGTGCA TCACCGTCAA CACCACCATC TGTGCCGGCT ACTGCCCCAC CATGACCCGC 301 GTGCTGCAGG GGGTCCTGCC GGCCCTGCCT CAGGTGGTGT GCAACTACCG CGATGTGCGC 361 TTCGAGTCCA TCCGGCTCCC TGGCTGCCCG CGCGGCGTGA ACCCCGTGGT CTCCTACGCC 421 GTGGCTCTCA GCTGTCAATG TGCACTCTGC CGCCGCAGCA CCACTTGCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA ATGCTTCTTA CTTAGTGCGT GGCGACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt