Construct: ORF TRCN0000478133
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003160.1_s317c1
- Derived from:
- ccsbBroadEn_04490
- DNA Barcode:
- ATTTGCTCCAATCACCCGAGGAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RSPH1 (89765)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478133
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89765 | RSPH1 | radial spoke head component 1 | NM_080860.4 | 99.8% | 68.6% | 634_635insC |
| 2 | human | 89765 | RSPH1 | radial spoke head component 1 | XM_011529786.1 | 92.1% | 60.8% | 501_502ins72;562_563insC |
| 3 | human | 89765 | RSPH1 | radial spoke head component 1 | NM_001286506.2 | 87.6% | 56.3% | 53_54ins114;520_521insC |
| 4 | human | 89765 | RSPH1 | radial spoke head component 1 | XM_005261208.2 | 77.3% | 45.6% | (many diffs) |
| 5 | mouse | 22092 | Rsph1 | radial spoke head 1 homolog... | NM_025290.3 | 76.9% | 63.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 714
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcggacctg ggctcggagg agttggagga ggagggagag aatgatattg 121 gggaatatga ggggggtcgg aatgaggcag gcgaaaggca cggacgtggg agggcacggc 181 tacccaacgg ggacacctac gaagggagct acgaattcgg taaaagacat ggccagggga 241 tctacaaatt taaaaatggt gctcgatata tcggagaata tgttagaaat aaaaagcacg 301 gtcaaggcac ttttatatat ccagatggat ccagatatga aggagagtgg gcaaatgacc 361 tgcggcacgg ccatggcgta tactactaca tcaataatga cacctacact ggagagtggt 421 ttgctcatca aaggcatggg caaggcacct atttatacgc ggagacgggc agtaagtatg 481 ttggcacctg ggtgaacgga cagcaggagg gcacggccga gctcattcac ctgaaccaca 541 ggtaccaggg caagttcttg aacaaaaatc ctgttggccc TGGAAAGTAT GTATTTGATG 601 TTGGGTGTGA ACAACATGGT GAATATCGTT TAACAGATAT GGAAAGAGGA GAAGAGGAAG 661 AGGAGGAAGA ATTAGTAACT GTTGTTCCAA AATGGAAAGC TACCCCAAAT CACTGAATTG 721 GCCCTGTGGA CACCAACTCT CCCCAAAAAG CCGACCTCTA CGGATGGACC TGGCCAAGAC 781 GCTCCAGGAG CTGAGAGTGC AGGAGAACCC GGGGAGGAGG CCCAGGCTCT GCTGGAGGGC 841 TTCGAGGGTG AGATGGACAT GAGGCCTGGA GATGAAGATG CAGACGTCCT CCGGGAAGAG 901 AGCCGGGAGT ATGACCAGGA GGAGTTCCGC TATGACATGG ATGAGGGAAA CATTAATTCT 961 GAAGAAGAAG AAACTAGACA GTCAGACCTC CAGGACTTGC CAACTTTCTT GTACAAAGTG 1021 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 1081 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 1141 GAATTTGCTC CAATCACCCG AGGAGTACGC GTTAAGTCga caatcaacct ctggattaca 1201 aaatttgtga aagatt