Transcript: Human XM_005261208.2

PREDICTED: Homo sapiens radial spoke head component 1 (RSPH1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RSPH1 (89765)
Length:
1070
CDS:
16..738

Additional Resources:

NCBI RefSeq record:
XM_005261208.2
NBCI Gene record:
RSPH1 (89765)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005261208.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000078718 CGAGAGGAGATCGTATCATAA pLKO.1 751 3UTR 100% 13.200 18.480 N RSPH1 n/a
2 TRCN0000078722 GCACCTATTTATACGCGGAGA pLKO.1 185 CDS 100% 2.160 3.024 N RSPH1 n/a
3 TRCN0000078721 GCGTATACTACTACATCAATA pLKO.1 116 CDS 100% 13.200 9.240 N RSPH1 n/a
4 TRCN0000419549 AGAGTGGTTTGCTCATCAAAG pLKO_005 153 CDS 100% 10.800 7.560 N RSPH1 n/a
5 TRCN0000415889 CAACATGGTGAATATCGTTTA pLKO_005 352 CDS 100% 10.800 7.560 N RSPH1 n/a
6 TRCN0000078720 CCTGGAAAGTATGTATTTGAT pLKO.1 319 CDS 100% 5.625 3.938 N RSPH1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005261208.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04490 pDONR223 100% 77.4% 77% None (many diffs) n/a
2 ccsbBroad304_04490 pLX_304 0% 77.4% 77% V5 (many diffs) n/a
3 TRCN0000478133 ATTTGCTCCAATCACCCGAGGAGT pLX_317 44.9% 77.3% 45.6% V5 (not translated due to prior stop codon) (many diffs) n/a
4 ccsbBroadEn_09271 pDONR223 100% 77.3% 77% None (many diffs) n/a
5 TRCN0000470604 GTCCCGTGCCATTATCAATGTGCA pLX_317 39.8% 77.3% 77% V5 (many diffs) n/a
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