Construct: ORF TRCN0000478179
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004088.1_s317c1
- Derived from:
- ccsbBroadEn_14713
- DNA Barcode:
- ATTAACTCACTCCAACAAGGAAAG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFIX (4784)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478179
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4784 | NFIX | nuclear factor I X | NM_001365982.2 | 65.3% | 64% | (many diffs) |
| 2 | human | 4784 | NFIX | nuclear factor I X | NM_001365985.2 | 59.9% | 59.3% | (many diffs) |
| 3 | human | 4784 | NFIX | nuclear factor I X | NM_002501.4 | 59.2% | 58% | (many diffs) |
| 4 | human | 4784 | NFIX | nuclear factor I X | NM_001271044.3 | 58.2% | 57.5% | (many diffs) |
| 5 | human | 4784 | NFIX | nuclear factor I X | XM_006722760.2 | 57% | 55.1% | (many diffs) |
| 6 | human | 4784 | NFIX | nuclear factor I X | NM_001365984.2 | 52.6% | 52% | (many diffs) |
| 7 | human | 4784 | NFIX | nuclear factor I X | NM_001365902.2 | 52% | 50.9% | (many diffs) |
| 8 | human | 4784 | NFIX | nuclear factor I X | NM_001271043.2 | 51.3% | 49.7% | (many diffs) |
| 9 | human | 4784 | NFIX | nuclear factor I X | XM_005259920.3 | 51.1% | 50.4% | (many diffs) |
| 10 | human | 4784 | NFIX | nuclear factor I X | XM_011528040.1 | 50.5% | 49% | (many diffs) |
| 11 | human | 4784 | NFIX | nuclear factor I X | XM_005259917.4 | 50.3% | 48.6% | (many diffs) |
| 12 | human | 4784 | NFIX | nuclear factor I X | NM_001365983.2 | 49.4% | 48.8% | (many diffs) |
| 13 | mouse | 18032 | Nfix | nuclear factor I/X | NM_010906.3 | 62.5% | 64% | (many diffs) |
| 14 | mouse | 18032 | Nfix | nuclear factor I/X | NM_001297601.1 | 61.5% | 63.5% | (many diffs) |
| 15 | mouse | 18032 | Nfix | nuclear factor I/X | NM_001081982.2 | 56.7% | 58% | (many diffs) |
| 16 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530761.3 | 49.8% | 50.9% | (many diffs) |
| 17 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530762.3 | 49.4% | 50.4% | (many diffs) |
| 18 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530763.3 | 49.1% | 49.9% | (many diffs) |
| 19 | mouse | 18032 | Nfix | nuclear factor I/X | NM_001081981.2 | 48.9% | 50.4% | (many diffs) |
| 20 | mouse | 18032 | Nfix | nuclear factor I/X | XM_011248326.2 | 46% | 47.1% | (many diffs) |
| 21 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530760.3 | 45.9% | 47% | (many diffs) |
| 22 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530759.3 | 42.7% | 43.7% | (many diffs) |
| 23 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530758.3 | 41.3% | 42.3% | (many diffs) |
| 24 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530757.3 | 41.2% | 42.2% | (many diffs) |
| 25 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530756.3 | 38.7% | 39.6% | (many diffs) |
| 26 | mouse | 18032 | Nfix | nuclear factor I/X | XM_006530755.3 | 38.6% | 39.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 864
- ORF length:
- 795
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctcccggct tgccgcctgc aggatgagtt ccacccgttc atcgaggcac 121 tgctgcctca cgtccgcgct ttctcctaca cctggttcaa cctgcaggcg cggaagcgca 181 agtacttcaa gaagcatgaa aagcggatgt cgaaggacga ggagcgggcg gtgaaggacg 241 agctgctggg cgagaagccc gagatcaagc agaagtgggc atcccggctg ctggccaagc 301 tgcgcaagga catccggccc gagttccgcg aggacttcgt gctgaccatc acgggcaaga 361 agcccccctg ctgcgtgctc tccaaccccg accagaaggg caagatccgg cggattgact 421 gcctgcgcca ggctgacaag gtgtggcggc tggacctggt catggtgatt ttgtttaagg 481 ggatccccct ggaaagtact gatggggagc ggctctacaa gtcgcctcag tgctcgaacc 541 ccggcctgtg cgtccagcca catcacattG GAGTCACAAT CAAAGAACTG GATCTTTATC 601 TGGCTTACTT TGTCCACACT CCGGAATCCG GACAATCAGA TAGTTCAAAC CAGCAAGGAG 661 ATGCGGACAT CAAACCACTG CCCAACGGGC ACTTAAGTTT CCAGGACTGT TTTGTGACTT 721 CCGGGGTCTG GAATGTGACG GAGCTGGTGA GAGTATCACA GACTCCTGTT GCAACAGCAT 781 CAGGGCCCAA CTTCTCCCTG GCGGACCTGG AGAGTCCCAG CTACTACAAC ATCAACCAGG 841 TGACCCCGGG ACGGCAACTT TCTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 901 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 961 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT TAACTCACTC 1021 CAACAAGGAA AGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 1081 tt