Construct: ORF TRCN0000478189
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008128.1_s317c1
- Derived from:
- ccsbBroadEn_01252
- DNA Barcode:
- GGCAGTTCATAACCTTCGCACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIA (5478)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478189
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5478 | PPIA | peptidylprolyl isomerase A | NM_021130.5 | 100% | 100% | |
| 2 | human | 729739 | PPIAP46 | peptidylprolyl isomerase A ... | NR_045207.1 | 93.5% | (many diffs) | |
| 3 | human | 653598 | PPIAL4C | peptidylprolyl isomerase A ... | NM_001135789.3 | 91.9% | 86% | (many diffs) |
| 4 | human | 653505 | PPIAL4A | peptidylprolyl isomerase A ... | NM_001143883.3 | 91.5% | 85.4% | (many diffs) |
| 5 | human | 644591 | PPIAL4G | peptidylprolyl isomerase A ... | NM_001123068.1 | 91.3% | 84.2% | (many diffs) |
| 6 | human | 105371242 | PPIAL4H | peptidylprolyl isomerase A ... | NM_001368128.1 | 91.1% | 84.2% | (many diffs) |
| 7 | human | 730262 | PPIAL4E | peptidylprolyl isomerase A ... | NM_001144032.2 | 90.9% | 84.2% | (many diffs) |
| 8 | human | 728945 | PPIAL4F | peptidylprolyl isomerase A ... | NM_001164262.2 | 90.9% | 84.2% | (many diffs) |
| 9 | human | 645142 | PPIAL4D | peptidylprolyl isomerase A ... | NM_001164261.1 | 90.7% | 83.6% | (many diffs) |
| 10 | human | 5478 | PPIA | peptidylprolyl isomerase A | XM_024446808.1 | 89% | 81.7% | (many diffs) |
| 11 | human | 100192204 | PPIAP30 | peptidylprolyl isomerase A ... | NR_036506.1 | 71.2% | (many diffs) | |
| 12 | human | 5478 | PPIA | peptidylprolyl isomerase A | NM_001300981.2 | 63.6% | 63.6% | 0_1ins180 |
| 13 | human | 5478 | PPIA | peptidylprolyl isomerase A | XM_024446809.1 | 63.6% | 63.6% | 0_1ins180 |
| 14 | human | 5478 | PPIA | peptidylprolyl isomerase A | XR_002956460.1 | 29% | 1_765del;952_1172del;1482_1704del | |
| 15 | human | 284996 | RNF149 | ring finger protein 149 | XR_001738713.2 | 16.5% | (many diffs) | |
| 16 | mouse | 268373 | Ppia | peptidylprolyl isomerase A | NM_008907.1 | 91.9% | 95.7% | (many diffs) |
| 17 | mouse | 668548 | Gm9234 | predicted pseudogene 9234 | XR_869917.3 | 60.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt caaccccacc gtgttcttcg acattgccgt cgacggcgag cccttgggcc 121 gcgtctcctt tgagctgttt gcagacaagg tcccaaagac agcagaaaat tttcgtgctc 181 tgagcactgg agagaaagga tttggttata agggttcctg ctttcacaga attattccag 241 ggtttatgtg tcagggtggt gacttcacac gccataatgg cactggtggc aagtccatct 301 atggggagaa atttgaagat gagaacttca tccTAAAGCA TACGGGTCCT GGCATCTTGT 361 CCATGGCAAA TGCTGGACCC AACACAAATG GTTCCCAGTT TTTCATCTGC ACTGCCAAGA 421 CTGAGTGGTT GGATGGCAAG CATGTGGTGT TTGGCAAAGT GAAAGAAGGC ATGAATATTG 481 TGGAGGCCAT GGAGCGCTTT GGGTCCAGGA ATGGCAAGAC CAGCAAGAAG ATCACCATTG 541 CTGACTGTGG ACAACTCGAA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGCA GTTCATAACC 721 TTCGCACTTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt