Transcript: Human XM_024446808.1

PREDICTED: Homo sapiens peptidylprolyl isomerase A (PPIA), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PPIA (5478)
Length:
1252
CDS:
547..1032

Additional Resources:

NCBI RefSeq record:
XM_024446808.1
NBCI Gene record:
PPIA (5478)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024446808.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049268 GCTCGCAGTATCCTAGAATCT pLKO.1 1107 3UTR 100% 4.950 3.465 N PPIAP31 n/a
2 TRCN0000049271 AGTGAAAGAAGGCATGAATAT pLKO.1 927 CDS 100% 13.200 7.920 N PPIAP31 n/a
3 TRCN0000049228 CTGACTGTGGACAACTCGAAT pLKO.1 1010 CDS 100% 4.950 2.970 N PPIA n/a
4 TRCN0000049231 GAATGGCAAGACCAGCAAGAA pLKO.1 978 CDS 100% 4.950 2.970 N PPIA n/a
5 TRCN0000049269 GCAAAGTGAAAGAAGGCATGA pLKO.1 923 CDS 100% 4.050 2.430 N PPIAP31 n/a
6 TRCN0000049229 GTTTGCAGACAAGGTCCCAAA pLKO.1 606 CDS 100% 4.050 2.430 N PPIA n/a
7 TRCN0000049169 GAGAACTTCATCCTAAAGCAT pLKO.1 790 CDS 100% 3.000 1.800 N PPIA n/a
8 TRCN0000049270 GAAGATGAGAACTTCATCCTA pLKO.1 784 CDS 100% 0.300 0.180 N PPIAP31 n/a
9 TRCN0000255862 TCCTGCTTTCACAGAATTATT pLKO_005 685 CDS 100% 15.000 7.500 Y PPIAL4C n/a
10 TRCN0000337286 AGAATTATTCCAGGGTTTATG pLKO_005 697 CDS 100% 13.200 6.600 Y PPIAL4A n/a
11 TRCN0000256034 CAGAATTATTCCAGGGTTTAT pLKO_005 696 CDS 100% 13.200 6.600 Y PPIAL4G n/a
12 TRCN0000337244 CCTGCTTTCACAGAATTATTC pLKO_005 686 CDS 100% 13.200 6.600 Y PPIAL4E n/a
13 TRCN0000255864 GGTGACTTCACACGCCATAAT pLKO_005 727 CDS 100% 13.200 6.600 Y PPIAL4C n/a
14 TRCN0000337285 GTGACTTCACACGCCATAATG pLKO_005 728 CDS 100% 13.200 6.600 Y PPIAL4A n/a
15 TRCN0000049232 GTTCCTGCTTTCACAGAATTA pLKO.1 683 CDS 100% 13.200 6.600 Y PPIA n/a
16 TRCN0000147390 GTTCCTGCTTTCACAGAATTA pLKO.1 683 CDS 100% 13.200 6.600 Y PPIAL4A n/a
17 TRCN0000431721 GCATCTTGTCCATGGCAAATG pLKO_005 821 CDS 100% 10.800 5.400 Y PPIAL4A n/a
18 TRCN0000377627 GGCATCTTGTCCATGGCAAAT pLKO_005 820 CDS 100% 10.800 5.400 Y PPIAL4A n/a
19 TRCN0000049230 TGGTGACTTCACACGCCATAA pLKO.1 726 CDS 100% 10.800 5.400 Y PPIA n/a
20 TRCN0000049168 ACCAGCAAGAAGATCACCATT pLKO.1 988 CDS 100% 4.950 2.475 Y PPIA n/a
21 TRCN0000049276 CAAATGCTGGACCCAACACAA pLKO.1 836 CDS 100% 4.950 2.475 Y PPIAP43 n/a
22 TRCN0000370864 CACTGGTGGCAAGTCCATCTA pLKO_005 750 CDS 100% 4.950 2.475 Y PPIAP80 n/a
23 TRCN0000049272 CATTGCTGACTGTGGACAACT pLKO.1 1005 CDS 100% 4.950 2.475 Y PPIAP31 n/a
24 TRCN0000049171 GATGGCAAGCATGTGGTGTTT pLKO.1 901 CDS 100% 4.950 2.475 Y PPIA n/a
25 TRCN0000049147 CAACACAAATGGTTCCCAGTT pLKO.1 849 CDS 100% 4.050 2.025 Y PPIAP55 n/a
26 TRCN0000146519 CAACACAAATGGTTCCCAGTT pLKO.1 849 CDS 100% 4.050 2.025 Y PPIAL4A n/a
27 TRCN0000049144 CACAGAATTATTCCAGGGTTT pLKO.1 694 CDS 100% 4.050 2.025 Y PPIAP55 n/a
28 TRCN0000049191 TGGCATCTTGTCCATGGCAAA pLKO.1 819 CDS 100% 4.050 2.025 Y LOC390299 n/a
29 TRCN0000049172 CTGGAGAGAAAGGATTTGGTT pLKO.1 656 CDS 100% 3.000 1.500 Y PPIA n/a
30 TRCN0000203719 CTGGAGAGAAAGGATTTGGTT pLKO.1 656 CDS 100% 3.000 1.500 Y LOC343384 n/a
31 TRCN0000049277 GCCAAGACTGAGTGGTTGGAT pLKO.1 883 CDS 100% 3.000 1.500 Y PPIAP43 n/a
32 TRCN0000365646 CATCTTGTCCATGGCAAATTC pLKO_005 822 CDS 100% 13.200 6.600 Y PPIAP80 n/a
33 TRCN0000337229 CATTGCTGACTGTGGACAATT pLKO_005 1005 CDS 100% 13.200 6.600 Y PPIAL4A n/a
34 TRCN0000101191 CCAGCAAGAAGATCACCATTT pLKO.1 989 CDS 100% 10.800 5.400 Y Ppia n/a
35 TRCN0000309763 CCAGCAAGAAGATCACCATTT pLKO_005 989 CDS 100% 10.800 5.400 Y Ppia n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024446808.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01252 pDONR223 100% 89% 81.7% None (many diffs) n/a
2 ccsbBroad304_01252 pLX_304 0% 89% 81.7% V5 (many diffs) n/a
3 TRCN0000478189 GGCAGTTCATAACCTTCGCACTTT pLX_317 46.2% 89% 81.7% V5 (many diffs) n/a
4 TRCN0000491637 CTCGAAGCCATCATTCTCCAGACG pLX_317 31.8% 89% 81.7% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroadEn_15534 pDONR223 0% 88.8% 81.1% None (many diffs) n/a
6 ccsbBroad304_15534 pLX_304 0% 88.8% 81.1% V5 (many diffs) n/a
7 TRCN0000473942 CGAGAGCTCGCACCGATCTTACTC pLX_317 69.1% 88.8% 81.1% V5 (many diffs) n/a
8 ccsbBroadEn_06756 pDONR223 100% 88.8% 81.1% None (many diffs) n/a
9 ccsbBroad304_06756 pLX_304 0% 88.8% 81.1% V5 (many diffs) n/a
10 TRCN0000467900 CATAACTCCTGAGAAGATCCAAAT pLX_317 64.8% 88.8% 81.1% V5 (many diffs) n/a
11 ccsbBroadEn_10188 pDONR223 100% 79.8% 72.5% None (many diffs) n/a
12 ccsbBroad304_10188 pLX_304 0% 79.8% 72.5% V5 (many diffs) n/a
13 TRCN0000480018 TCTCTTGGGAGGTGCATTCGATCA pLX_317 83.1% 79.8% 72.5% V5 (many diffs) n/a
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