Construct: ORF TRCN0000478217
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015033.1_s317c1
- Derived from:
- ccsbBroadEn_12111
- DNA Barcode:
- CCTCGCCACTGTCCTGTGGCCGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHTF8 (54921)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478217
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001002847.3 | 8.3% | 8.3% | 1_1440del |
| 2 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001040144.2 | 8.3% | 8.3% | 1_1440del |
| 3 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001366603.1 | 8.3% | 8.3% | 1_1440del |
| 4 | human | 113455421 | DERPC | DERPC proline and glycine r... | NM_001366604.1 | 8.3% | 8.3% | 1_1440del |
| 5 | mouse | 214987 | Chtf8 | CTF8, chromosome transmissi... | XM_017312654.1 | 7.5% | 7.5% | (many diffs) |
| 6 | mouse | 214987 | Chtf8 | CTF8, chromosome transmissi... | XM_017312655.1 | 7.5% | 7.5% | (many diffs) |
| 7 | mouse | 214987 | Chtf8 | CTF8, chromosome transmissi... | XM_017312656.1 | 7.5% | 7.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 222
- ORF length:
- 132
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAATGGCCC TGCAGGCAAG AGTTTCGTCC 121 CATTTCCTAG AGTGGGGAGC CTCCCTGGCA CAAACCCAGC TGCTTTCCCC AGACCAGGGG 181 GTCCAATGGC TGCAATGTAC CCAAATGGAA TGTTGCCCCC TTACCCAACT TTCTTGTACA 241 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 301 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 361 AGGACGACCT CGCCACTGTC CTGTGGCCGG CACGCGTTAA GTCgacaatc aacctctgga 421 ttacaaaatt tgtgaaagat t