Construct: ORF TRCN0000478265
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015909.1_s317c1
- Derived from:
- ccsbBroadEn_08714
- DNA Barcode:
- GCACACGGTGTACATGGGCGCGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PELI1 (57162)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478265
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | NM_020651.4 | 99.8% | 100% | 243A>G;291C>A |
| 2 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532994.3 | 99.8% | 100% | 243A>G;291C>A |
| 3 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004520.1 | 99.8% | 100% | 243A>G;291C>A |
| 4 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_017004521.2 | 83.9% | 83.9% | (many diffs) |
| 5 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_005264437.3 | 76.5% | 76.5% | 0_1ins294 |
| 6 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532996.2 | 76.5% | 76.5% | 0_1ins294 |
| 7 | human | 57162 | PELI1 | pellino E3 ubiquitin protei... | XM_011532997.2 | 57.1% | 57.1% | 0_1ins537 |
| 8 | mouse | 67245 | Peli1 | pellino 1 | NM_023324.2 | 92.8% | 99.7% | (many diffs) |
| 9 | mouse | 67245 | Peli1 | pellino 1 | XM_011243746.1 | 78.4% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1320
- ORF length:
- 1254
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ttctcctgat caagaaaatc atccatctaa agcaccagta aaatatggtg 121 aactcattgt cttagggtat aatgggtctc tcccaaatgg cgatagagga aggaggaaaa 181 gtaggtttgc tttgtttaaa agacctaagg caaatggggt gaagcccagc actgtgcata 241 ttgcttgtac tcctcaggct gcaaaggcaa taagcaacaa agaccagcat agcatatcat 301 atactttgtc tcgggcccag actgtggtgg ttgaatatac tcatgacagc aacacagata 361 tgtttcagat tggccggtcg actgaaagcc ccattgattt tgtagtaact gacacggttc 421 ctggaagtca aagtaattct gatacacagt cagtacaaag cactatatca agatttgcct 481 gcagaatcat atgtgaacgg aatcctccct ttacagcacg gatttatgct gcaggatttg 541 actcatcaaa aaacatcttt cttggggaga aggctgccaa atggaagaca tcagatggac 601 agatggatgg cttgaccact aatggtgttc ttgtgatgca tccacgcaat gggttcacag 661 aagactccaa gcctggaata tggagagaaa tatcggtgtg tggaaatgta tttagcctac 721 gtgaaaccag atcggctcag cagagaggaa aaatggtgga aattgaaacc aatcagttac 781 aagatggctc gttaattgac ctctgtggtg caacattgtt atggcgtact gcagaaggcc 841 tttcccacac tcctaccgtg aagcatttag aagctttaag acaggaaatc aatgcagcac 901 gacctcagtg ccctgtaggg ttcaacacac tagcatttcc tagtatgaag aggaaagacg 961 ttgtagatga aaaacaacca tgggtatatc taaactgcgg ccatgtacat ggctatcata 1021 actggggaaa caaagaagaa cgtgatggaa aagatcgtga atgtcctatg tgtaggtctg 1081 ttggtcccta tgttcctctg tggcttGGAT GTGAAGCTGG ATTTTATGTG GACGCCGGCC 1141 CTCCAACCCA TGCGTTTAGC CCGTGTGGGC ATGTGTGTTC AGAAAAGACA ACTGCCTATT 1201 GGTCCCAGAT CCCACTTCCT CATGGTACTC ATACTTTTCA TGCAGCCTGT CCCTTTTGTG 1261 CACATCAGTT GGCTGGTGAA CAAGGCTACA TCAGACTTAT TTTTCAAGGA CCTCTAGACT 1321 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGAGCACA CGGTGTACAT GGGCGCGGAA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt