Construct: ORF TRCN0000478302
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010998.1_s317c1
- Derived from:
- ccsbBroadEn_08522
- DNA Barcode:
- CATATGCGTTCGCTCCAACCTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYNJ2BP (55333)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478302
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55333 | SYNJ2BP | synaptojanin 2 binding protein | NM_018373.3 | 99.7% | 100% | 424C>A |
| 2 | human | 100529257 | SYNJ2BP-COX16 | SYNJ2BP-COX16 readthrough | NM_001202549.2 | 75.4% | 52.3% | (many diffs) |
| 3 | human | 100529257 | SYNJ2BP-COX16 | SYNJ2BP-COX16 readthrough | NM_001202547.2 | 72.5% | 69.1% | (many diffs) |
| 4 | human | 100529257 | SYNJ2BP-COX16 | SYNJ2BP-COX16 readthrough | NM_001202548.2 | 62.8% | 50.4% | (many diffs) |
| 5 | mouse | 24071 | Synj2bp | synaptojanin 2 binding protein | NM_025292.7 | 89.1% | 91% | (many diffs) |
| 6 | mouse | 105940408 | Gm20498 | predicted gene 20498 | NM_001309848.1 | 71.5% | 57.5% | (many diffs) |
| 7 | mouse | 24071 | Synj2bp | synaptojanin 2 binding protein | NM_001309814.1 | 71.2% | 60% | (many diffs) |
| 8 | mouse | 105940408 | Gm20498 | predicted gene 20498 | NM_001309847.1 | 61.9% | 49.5% | (many diffs) |
| 9 | mouse | 24071 | Synj2bp | synaptojanin 2 binding protein | NR_132136.1 | 2.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cggaagagtg gattatttgg tcactgagga agagatcaat cttaccagag 121 ggccctcagg gctgggcttc aacatcgtcg gtgggacaga tcagcagtat gtctccaacg 181 acagtggcat cTACGTCAGC CGCATCAAAG AAAATGGGGC TGCGGCCCTG GATGGGCGGC 241 TCCAGGAGGG TGATAAGATC CTTTCGGTAA ATGGCCAAGA CCTAAAGAAC CTGCTGCACC 301 AGGATGCTGT AGACCTCTTT CGTAATGCAG GCTATGCTGT GTCTCTGAGA GTGCAGCACA 361 GGTTACAGGT GCAGAATGGA CCTATAGGAC ATCGAGGTGA AGGGGACCCA AGTGGTATTC 421 CCATATTTAT GGTGCTGGTG CCAGTGTTTG CCCTCACCAT GGTAGCAGCC TGGGCTTTCA 481 TGAGATACAG GCAACAACTT TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACATA TGCGTTCGCT 661 CCAACCTGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt