Construct: ORF TRCN0000478316
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007678.1_s317c1
- Derived from:
- ccsbBroadEn_15913
- DNA Barcode:
- TCATCTGTGTCTAATATGCCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGK (55750)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478316
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55750 | AGK | acylglycerol kinase | NM_001364948.2 | 17.2% | 14% | (many diffs) |
| 2 | human | 55750 | AGK | acylglycerol kinase | NM_018238.4 | 14.2% | 11.6% | (many diffs) |
| 3 | human | 55750 | AGK | acylglycerol kinase | XM_011516397.3 | 14.2% | 11.6% | (many diffs) |
| 4 | human | 55750 | AGK | acylglycerol kinase | XM_024446835.1 | 14.2% | 11.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 285
- ORF length:
- 195
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGACGGTGTT CTTTAAAACG CTTCGAAATC 121 ACTGGAAGAA AACTACAGCT GGGCTCTGCC TGCTGACCTG GGGAGGCCAT TGGCTCTATG 181 GAAAACACTG TGATAACCTC CTAAGGAGAG CAGCCTGTCA AGAAGCTCAG CACTATCAGG 241 ATGAATCACG CTGGGAGCCA ACTCTGAGTA GAACTCCAGG AAGTTACCCA ACTTTCTTGT 301 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 361 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 421 GAAAGGACGA TCATCTGTGT CTAATATGCC CTTCACGCGT TAAGTCgaca atcaacctct 481 ggattacaaa atttgtgaaa gatt