Transcript: Human XM_011516397.3

PREDICTED: Homo sapiens acylglycerol kinase (AGK), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AGK (55750)
Length:
11135
CDS:
638..1906

Additional Resources:

NCBI RefSeq record:
XM_011516397.3
NBCI Gene record:
AGK (55750)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146482 GCCAGATAAATGTAAAATCG pXPR_003 GGG 259 20% 5 0.6533 AGK AGK 76155
2 BRDN0001147162 TGTTCTTCGACGAACAGATG pXPR_003 AGG 418 33% 7 0.4025 AGK AGK 76153
3 BRDN0001146005 CAAAGAAGGCCTTTGTACAG pXPR_003 GGG 808 64% 12 -0.2286 AGK AGK 76154
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011516397.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358551 CCCTCCTAAACACGGACTTTC pLKO_005 2132 3UTR 100% 10.800 15.120 N AGK n/a
2 TRCN0000153828 CCTCAACTGTACTTGGAGAAA pLKO.1 2643 3UTR 100% 4.950 3.960 N AGK n/a
3 TRCN0000236010 TCCATCACAACACGGAATAAT pLKO_005 1577 CDS 100% 15.000 10.500 N AGK n/a
4 TRCN0000236013 AGCTAGTTCTTTAGTAGTTAA pLKO_005 2955 3UTR 100% 13.200 9.240 N AGK n/a
5 TRCN0000358622 CATCAAGCCTCTATCTCATAC pLKO_005 1379 CDS 100% 10.800 7.560 N AGK n/a
6 TRCN0000152281 CCCAAACCTAATAACCCAAAT pLKO.1 2550 3UTR 100% 10.800 7.560 N AGK n/a
7 TRCN0000358552 TCAGAGATGCTGGCGTCAAAG pLKO_005 1278 CDS 100% 10.800 7.560 N AGK n/a
8 TRCN0000152111 CAGTAAGATTCCCATTGGATT pLKO.1 1069 CDS 100% 4.950 3.465 N AGK n/a
9 TRCN0000153540 CCATTGAACTGTCCATCACAA pLKO.1 1566 CDS 100% 4.950 3.465 N AGK n/a
10 TRCN0000156414 CCACCATTGAACTGTCCATCA pLKO.1 1563 CDS 100% 4.050 2.835 N AGK n/a
11 TRCN0000157186 GAAGCTCAGGTGTTTGGCAAT pLKO.1 770 CDS 100% 4.050 2.835 N AGK n/a
12 TRCN0000236009 ATCAGCAAAGGAGACTTTATA pLKO_005 1655 CDS 100% 15.000 9.000 N AGK n/a
13 TRCN0000236012 TAAGATTCCCATTGGATTTAT pLKO_005 1072 CDS 100% 15.000 9.000 N AGK n/a
14 TRCN0000236011 TGGAAACAAAGTCCAACATAT pLKO_005 1141 CDS 100% 13.200 7.920 N AGK n/a
15 TRCN0000361857 TGGAAACAAAGTCCAACATAT pLKO_005 1141 CDS 100% 13.200 7.920 N Agk n/a
16 TRCN0000153242 GCCCTTCCATTTCTCTTCTTT pLKO.1 2246 3UTR 100% 5.625 3.375 N AGK n/a
17 TRCN0000024801 CCCAATGCACAAGTGAAGAAA pLKO.1 803 CDS 100% 5.625 3.938 N Agk n/a
18 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 4695 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011516397.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08577 pDONR223 100% 99.9% 100% None 228C>T n/a
2 ccsbBroad304_08577 pLX_304 0% 99.9% 100% V5 228C>T n/a
3 TRCN0000471201 TCAACACGTGCAGTGTTACCCGGG pLX_317 33% 99.9% 100% V5 228C>T n/a
4 ccsbBroadEn_15107 pDONR223 0% 99.9% 100% None 228C>T n/a
5 TRCN0000472791 ATAGCGTTCTCAGCCTTGGACTTT pLX_317 32.8% 99.9% 100% V5 228C>T n/a
6 ccsbBroadEn_15913 pDONR223 0% 14.2% 11.6% None (many diffs) n/a
7 ccsbBroad304_15913 pLX_304 0% 14.2% 11.6% V5 (many diffs) n/a
8 TRCN0000478316 TCATCTGTGTCTAATATGCCCTTC pLX_317 100% 14.2% 11.6% V5 (many diffs) n/a
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