Construct: ORF TRCN0000478319
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009922.1_s317c1
- Derived from:
- ccsbBroadEn_04880
- DNA Barcode:
- CCTGGCCTTGTAGCACGTATTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSMEM2 (132228)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478319
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 132228 | LSMEM2 | leucine rich single-pass me... | NM_153215.3 | 100% | 100% | |
| 2 | human | 132228 | LSMEM2 | leucine rich single-pass me... | NM_001304385.2 | 99.3% | 99.3% | 170_171insAGC |
| 3 | human | 132228 | LSMEM2 | leucine rich single-pass me... | XM_006712979.4 | 87.2% | 84.2% | (many diffs) |
| 4 | human | 132228 | LSMEM2 | leucine rich single-pass me... | XM_006712980.4 | 86.7% | 83.7% | (many diffs) |
| 5 | human | 132228 | LSMEM2 | leucine rich single-pass me... | XM_011533370.3 | 85.3% | 85.3% | 0_1ins72 |
| 6 | human | 132228 | LSMEM2 | leucine rich single-pass me... | XM_024453354.1 | 85.3% | 85.3% | 0_1ins72 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc atcattggcc cccgactgcc cactgcttgc catgcctgag gagacccaag 121 aagactccgt ggcgccaatg atgcccagcc agaggagcag ggggccattg gcccccaacc 181 acgtgcatga ggtatgcctg caccaggtgg agtccatcag cgaccTACAT AGTGGAGCAG 241 GCACACTGCG CCCCTATCTA ACTGAAGAGG CACGACCGTG GGATGAGCTG CTGGGCGTTT 301 TGCCGCCGTC ACTGTGTGCC CAGGCTGGCT GCAGCCCTGT GTACAGACGA GGAGGGTTCC 361 TGCTGCTGCT CGCGCTGCTG GTGCTCACTT GCCTAGTGCT CGCACTCCTG GCTGTCTACC 421 TGAGCGTGCT GCAGAGTGAA TCCCTGCGCA TCCTGGCACA CACGCTCCGC ACGCAGGAGG 481 AGACACTACT CAAACTCCGC TTGGCCAGCC TCAGCCAGCT TCGGAGGCTC AACTCCAGTG 541 AGGCCCAAGC ACCCAGCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCTGGCC TTGTAGCACG 721 TATTTCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt